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Fluorescent Multiplex Kits (Rapid)
The AssayLiteTM Rapid series is based on the Competitive Immunoassay principles.
Step 1: Two different type of antibody coated on the plate uniformly and the standard/samples added along with fluoresent labeled protein into the well
Step 2: Fluorescent labeled protein compete with none labeled protein binding to the antibody. Blank has highest fluoresent signal and the signal decrease when none labeled protein concentration increase.