Human Tissue-type Plasminogen Activator (tPA) Activity Assay Kit

Preclinical assessment of adjunctive tPA and DNase for peritoneal dialysis associated peritonitis

• https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0119238
• AL McGuire, SC Bennett, SM Lansley, ND Popowicz - PloS one, 2015 - journals.plos.org
• tPA activity was determined using a chromogenic tPA assay (Human tPA Chromogenic Activity Kit, CT1001, AssayPro USA) that measures the tPA-mediated cleavage of the zymogen

CLOT BUSTER BULLETS: THE CREATION OF TPA COATED NANOPARTICLES

• http://msoe.s3.amazonaws.com/files/resources/stasinopoulos-no-heading-paper.pdf
• N Stasinopoulos - msoe.s3.amazonaws.com
• The new tPA-dendrimer complexes were tested with a chromomeric assay purchased from AssayPro in St Charles, MO (CT1001). Once mixed with the assay reactants and the tPA

Fish Protein Stimulated the Fibrinolysis in Rats

• https://pubmed.ncbi.nlm.nih.gov/15542926/
• Murata M et al. (2004). Ann Nutr Metab. 48(5):348-356
• No Snippet Provided

Consumption of yogurt enriched with polar lipids from olive oil by-products reduces platelet sensitivity against platelet activating factor and inflammatory indices: A randomized, double-blind clinical trial

• https://www.sciencedirect.com/science/article/pii/S2666149722000081
• Antonopoulou S et al. (2022). Human Nutrition & Metabolism 28:200145.
• No Snippet Provided

Design and production of new chimeric reteplase with enhanced fibrin affinity: a theoretical and experimental study

• https://www.researchgate.net/profile/Karim-Mahnam/publication/339511723_Design_and_production_of_new_chimeric_reteplase_with_enhanced_fibrin_affinity_a_theoretical_and_experimental_study/links/5eb8e33e4585152169c57f0f/Design-and-production-of-new-chimeric-reteplase-with-enhanced-fibrin-affinity-a-theoretical-and-experimental-study.pdf
• Mohammadi E et al. (2021). J Biomol Struct Dyn. 39(4):1321-1333.
• No Snippet Provided

Producing if Tenecteplase with High Activity

• https://onlinelibrary.wiley.com/doi/epdf/10.1002/rth2.12589
• Chashchinova D et al. (2021). Researh & Practice in Thrombosis & Haemostasis. 5(2). DOI: 10.1002/rth2.12589.
• No Snippet Provided

Alu-repeat polymorphism in the tissue plasminogen activator (t-PA) gene, seminal t-PA concentration, and male fertility impairment: A case-control study

• https://pmc.ncbi.nlm.nih.gov/articles/PMC7457157/pdf/ijrb-18-571.pdf
• Tahtamouni LH et al. (2020). Int J Reprod Biomed. 18(8):571-578.
• No Snippet Provided

Optimization of Fermentation Conditions for Reteplase Expression by Escherichia coli Using Response Surface Methodology

• https://pmc.ncbi.nlm.nih.gov/articles/PMC6490408/pdf/AJMB-11-162.pdf
• Zare H et al. (2019). Avicenna J Med Biotechnol. 11(2):162-168.
• No Snippet Provided

Improvement of Soluble Production of Reteplase in Escherichia coli by Optimization of Chemical Chaperones in Lysis Buffer

• https://pmc.ncbi.nlm.nih.gov/articles/PMC6839271/pdf/ABR-8-65.pdf
• Fazeli B et al. (2019). Adv Biomed Res. 8:65.
• No Snippet Provided

Short chain fatty acids induce tissue plasminogen activator in airway epithelial cells via GPR 41&43

• https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987538/pdf/nihms968073.pdf
• Imoto Y et al. (2018). Clin Exp Allergy. 48(5): 544-554.
• No Snippet Provided

Effect of buffer additives on solubilization and refolding of reteplase inclusion bodies

• https://pmc.ncbi.nlm.nih.gov/articles/PMC6082031/pdf/RPS-13-413.pdf
• Esmaili I et al. (2018). Res Pharm Sci. 13(5):413-421.
• No Snippet Provided

Ginkgo biloba Extract (EGb 761) Inhibits Glutamate-induced Up-regulation of Tissue Plasminogen Activator Through Inhibition of c-Fos Translocation in Rat Primary Cortical Neurons

• https://pubmed.ncbi.nlm.nih.gov/26478151/#full-view-affiliation-1
• Cho KS et al. (2016). Phytother Res. 30(1):58-65.
• No Snippet Provided

Preclinical Assessment of Adjunctive tPA and DNase for Peritoneal Dialysis Associated Peritonitis

• https://pmc.ncbi.nlm.nih.gov/articles/PMC4351066/pdf/pone.0119238.pdf
• McGuire AL et al. (2015). PLoS One. 10(3):e0119238.
• No Snippet Provided

Phloretin suppresses thrombin-mediated leukocyte-platelet-endothelial interactions

• https://pubmed.ncbi.nlm.nih.gov/24338998/
• Kim MS et al.(2014).Mol Nutr Food Res. 58(4):698-708.
• No Snippet Provided

Design of a novel chimeric tissue plasminogen activator with favorable Vampire bat plasminogen activator properties

• https://www.researchgate.net/profile/Farzaneh-Barkhordari-2/publication/265910155_Design_of_a_novel_chimeric_tissue_plasminogen_activator_with_favorable_Vampire_bat_plasminogen_activator_properties/links/5afc0f04aca272e7302cab4a/Design-of-a-novel-chimeric-tissue-plasminogen-activator-with-favorable-Vampire-bat-plasminogen-activator-properties.pdf
• Kazemali MR et al. (2014). Enzyme Microb Technol. 67:82-6.
• No Snippet Provided

Resveratrol down-regulates a glutamate-induced tissue plasminogen activator via Erk and AMPK/mTOR pathways in rat primary cortical neurons

• https://pubmed.ncbi.nlm.nih.gov/24599349/#full-view-affiliation-1
• Cho KS et al. (2014). Food Funct.5(5):951-60.
• No Snippet Provided

Cathepsin D stimulates the activities of secreted plasminogen activators in the breast cancer acidic environment

• http://www.spandidos-publications.com/ijo/43/5/1683
• Maynadier M et al. (2013). Int J Oncol. 43(5):1683-90
• No Snippet Provided

Inhibitors of intracellular urokinase plasminogen activator and methods of use thereof.

• https://patents.google.com/patent/WO2009020877A2/en
• Gorin FA et al. (2013)
• No Snippet Provided

Myxomavirus-derived Serpin Prolongs Survival and Reduces Inflammation and 2 Hemorrhage in an Unrelated Lethal Mouse Viral Infection

• https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3754305/pdf/zac4114.pdf
• Chen H et al. (2013). Antimicrob Agents Chemother. 57(9):4114-4127
• No Snippet Provided

Valproic acid induces astrocyte-dependent neurite outgrowth from cultured rat primary cortical neuron via modulation of tPA/PAI-1 activity

• https://pubmed.ncbi.nlm.nih.gov/23378038/#full-view-affiliation-1
• Cho KS et al. (2013). Glia. 61(5):694-709.
• No Snippet Provided

TWEAK/Fn14 interaction enhances PAI-1 and tissue factor expression in atherosclerotic plaques and in cultured vascular smooth muscle cells

• http://cardiovascres.oxfordjournals.org/content/89/1/225.long
• Munoz-Garcia1 B (2011). Cardiovasc Res. 89(1):225-233
• No Snippet Provided

Annexin A2 Is a Molecular Target for TM601, a Peptide with Tumor-targeting and Anti-angiogenic Effects

• http://www.jbc.org/content/285/7/4366.full.pdf+html
• Kesavan K et al. (2010). J Biol Chem. 285(7):4366-4374
• No Snippet Provided

Characterization and Usefulness of Clot-Fibrinolysis Waveform Analysis in Critical Care Patients with Enhanced or Suppressed Fibrinolysis.

• https://pubmed.ncbi.nlm.nih.gov/37527783/
• Takumi Tsuchida, Mineji Hayakawa, Osamu Kumano (2024). Thrombosis and haemostasis. 2024 Jan 15
• Human Tissue-type Plasminogen Activator (tPA) Chromogenic AssaySense Activity Assay Kit and Human Plasminogen Activator Inhibitor-1 (PAI-1) Chromogenic AssaySenseActivity AssayKit were purchased from ASSAYPRO (St. Charles, Missouri, United States) and used. tPA/PAI-1 ratios and their concentrations were also calculated to express thebalancebetween enhanced and suppressed fibrinolysis status.

Relationship Between Severity of Fibrinolysis Based on Rotational Thromboelastometry and Conventional Fibrinolysis Markers

• https://pubmed.ncbi.nlm.nih.gov/32571089/
• TomoyoTomoyo Saito Saito, MinejiMineji Hayakawa Hayakawa, Tomonao Yoshida (2020). Clinical and Applied Thrombosis/Hemostasis. 2020 Jun 22
• These variables were measured at the Central Laboratory for Clinical Chemistry...The t-PA activity was measured using a commercial Chromogenic Activity kit (Human tPA Chromogenic Activity Kit, Assaypro LLC).

Design and production of new chimeric reteplase with enhanced fibrin affinity: a theoretical and experimental study

• https://pubmed.ncbi.nlm.nih.gov/32098615/
• Elmira Mohammadi, Karim Mahnam (2020). Journal of Biomolecular Structure & Dynami…. 2020 Mar 02
• Abstract: Plasminogen activators (PAs) are widely used for treatment of disorders caused by clot formation...Fibrin specific PAs are safe drugs from this group because of reducing the incidence of hem…

Optimization of Fermentation Conditions for Reteplase Expression by Escherichia coli Using Response Surface Methodology

• https://pubmed.ncbi.nlm.nih.gov/31057718/
• Hamze Zare, Hamid Mir Mohammad Sadeghi, Vajihe Akbari (2019). Avicenna Journal of Medical Biotechnology. 2019 Jan 01
• The sample was purified using Ni-NTA affinity column, as described previously....Then, 20 μl of the sample was applied for determining biological activity by Assay Sense Human tPA Chromogenic Activity Kit (Assaypro, USA)...The commercial reteplase (Retelies®) was used as a positive control and the buffer as...

Improvement of Soluble Production of Reteplase in Escherichia coli by Optimization of Chemical Chaperones in Lysis Buffer

• https://pubmed.ncbi.nlm.nih.gov/31737582/
• Behnaz Fazeli, Vajihe Akbari, Hamid Mir Mohammad Sadeghi (2019). Advanced Biomedical Research. 2019 Jan 01
• ...three times, and the blots were visualized via incubation with a chromogenic substrate, 3,3'-diaminobenzidine...The biological activity of obtained protein was evaluated using AssaySense Human tPA Chromogenic Activity Kit (Assaypro, USA) as described previously...[ ] The standard reteplase (Retelies®) and the buffer were used as a positive...

Effect of buffer additives on solubilization and refolding of reteplase inclusion bodies

• https://pubmed.ncbi.nlm.nih.gov/30271443/
• Iman Esmaili, Hamid Mir Mohammad Sadeghi, Vajihe Akbari (2018). Research in Pharmaceutical Sciences. 2018 Oct 01
• ...by densitometry analysis of corresponding band using TL120 software (nonlinear Inc, Durham nc, USA)...Bioactivity of refolded reteplase Activation of plasminogen by reteplase was determined by AssaySense Human tPA Chromogenic Activity Kit (Assaypro, USA)...To 20 μL of refolded samples, was added 80 μL of assay mix (50...

Short-chain fatty acids induce tissue plasminogen activator in airway epithelial cells via GPR41 43

• https://pubmed.ncbi.nlm.nih.gov/29431874/
• Y Imoto, A Kato,...., R P Schleimer (2018). Clinical and experimental allergy : journa…. 2018 Mar 24
• ...Reader (Bio-Rad, Hercules, CA) with associated software applied to the sandwich enzyme immunoassay technique...The activity of t-PA was determined with a commercially available Human tPA Chromogenic Activity Assay Kit according to the manufacturer's protocol (Assaypro)...The absorbance was measured using the Bio-Rad Spectrophotometer Model 680 Microplate Reader (Bio-Rad).

Ginkgo biloba Extract (EGb 761®) Inhibits Glutamate-induced Up-regulation of Tissue Plasminogen Activator Through Inhibition of c-Fos Translocation in Rat Primary Cortical Neurons.

• https://pubmed.ncbi.nlm.nih.gov/26478151/
• Kyu Suk Cho, Ian Myungwon Lee,...., Seol-Heui Han (2016). Phytotherapy research : PTR. 2016 Jun 21
• In the study, the Human Tissue type Plasminogen Activator tPA Chromogenic AssaySense Activity Assay Kit from Assaypro was pivotal for measuring tPA activity in rat primary cortical neu…

Phloretin suppresses thrombin-mediated leukocyte-platelet-endothelial interactions.

• https://pubmed.ncbi.nlm.nih.gov/24338998/
• Min Soo Kim, Sin-Hye Park,...., Young-Hee Kang (2015). Molecular nutrition & food research. 2015 Nov 02
• In this study, the Human Tissue type Plasminogen Activator tPA Chromogenic AssaySense Activity Assay Kit from Assaypro played a crucial role by enabling the measurement of tPA activity…

Preclinical Assessment of Adjunctive tPA and DNase for Peritoneal Dialysis Associated Peritonitis

• https://pubmed.ncbi.nlm.nih.gov/25742006/
• Amanda L. McGuire, Sophia C. Bennett, Aron Chakera (2015). PLoS ONE. 2015 Mar 05
• The remaining samples were incubated at 37°C in a waterbath for 6 hours then frozen at -20°C (T = 6 samples). tPA activity was determined using a chromogenic tPA assay (Human tPA Chromogenic Activity Kit, CT1001, AssayPro USA) that measures the tPA-mediated cleavage of the zymogen plasminogen into the active serine protease plasmin, which digests fibrin [ ].

Resveratrol down-regulates a glutamate-induced tissue plasminogen activator via Erk and AMPK/mTOR pathways in rat primary cortical neurons.

• https://pubmed.ncbi.nlm.nih.gov/24599349/
• Kyu Suk Cho, Eun Joo Lee,...., Chan Young Shin (2014). Food & Function. 2014 Dec 19
• In this study, the Human Tissue type Plasminogen Activator tPA Chromogenic AssaySense Activity Assay Kit from Assaypro played a significant role in measuring tPA activity in rat primar…

Design of a novel chimeric tissue plasminogen activator with favorable Vampire bat plasminogen activator properties

• https://pubmed.ncbi.nlm.nih.gov/25442953/
• MohammadReza Kazemali, Keivan Majidzadeh-A,...., Fereidoun Mahboudi (2014). Enzyme and microbial technology. 2014 Dec 01
• Abstract: Fibrinolytic agents are widely used in treatment of the thromboembolic disorders. The new generations like recombinant tissue plasminogen activator (t-PA, alteplase) are not showing promisin…

Valproic acid induces astrocyte-dependent neurite outgrowth from cultured rat primary cortical neuron via modulation of tPA/PAI-1 activity.

• https://pubmed.ncbi.nlm.nih.gov/23378038/
• Kyu Suk Cho, Kyoung Ja Kwon,...., Chan Young Shin (2013). Glia. 2013 Dec 16
• In the study, the Human Tissue type Plasminogen Activator tPA Chromogenic AssaySense Activity Assay Kit from Assaypro played an essential role in measuring tPA activity in culture supe…

Myxomavirus-Derived Serpin Prolongs Survival and Reduces Inflammation and Hemorrhage in an Unrelated Lethal Mouse Viral Infection

• https://pubmed.ncbi.nlm.nih.gov/23774438/
• Hao Chen, Donghang Zheng,...., Alexandra Lucas (2013). Antimicrobial Agents and Chemotherapy. 2013 Sep 23
• Tris, 150 mM imidazole, 150 mM NaCl). NSP was dialyzed into 0.9% saline and concentrated using a 10-kDa-cutoff Amicon Ultra (Millipore) and then passed through a 0.22-μm filter (Millipore). A single band was also detected for NSP by gel electrophoresis and inhibitory activity measured for uPA or tPA by chromogenic assay (data not shown; Assaypro, St. Louis, MO) ( 14 , 15 , 17 – 21 , 24 ).

Cathepsin D stimulates the activities of secreted plasminogen activators in the breast cancer acidic environment

• https://pubmed.ncbi.nlm.nih.gov/24026424/
• MARIE MAYNADIER, REZA FARNOUD (2013). International Journal of Oncology. 2013 Sep 10
• Plasminogen activator enzymatic assay...PA activity in conditioned media was determined in triplicate using the human PA chromogenic enzymatic kit (Assay pro ref CT1001, AssaySense, St. Charles, MO, USA) according to instructions of this laboratory...This assay was not specific for tPA or uPA.

Expression of the recombinant plasminogen activator (reteplase) by a non-lytic insect cell expression system

• https://pubmed.ncbi.nlm.nih.gov/24459471/
• S. Aflakiyan, H. Mir Mohammad Sadeghi, A. Jahanian-Najafabadi (2013). Research in Pharmaceutical Sciences. 2013 Jan 01
• ...expression of the reteplase protein, HRP conjugated anti His-tag antibody (Roche, Germany) was used...Assessment of biological activity The biological activity of the expressed reteplase was evaluated using AssaySense Human tPA Chromogenic Activity Assay Kit (Assaypro, MO, USA) according to the manufacturer's instructions...The assay measures the ability of tPA to convert the plasminogen to plasmin in...

The High-Level Expression of Human Tissue Plasminogen Activator in the Milk of Transgenic Mice with Hybrid Gene Locus Strategy

• https://pubmed.ncbi.nlm.nih.gov/21688038/
• Yanrong Zhou, Yanli Lin (2011). Molecular biotechnology. 2011 Jun 18
• Abstract: Transgene expression for the mammary gland bioreactor aimed at producing recombinant proteins requires optimized expression vector construction. Previously we presented a hybrid gene locus s…

Anti-t-PA antibodies in acute myocardial infarction after thrombolysis with rt-PA

• https://pubmed.ncbi.nlm.nih.gov/20122609/
• Massimo Cugno, Roberto Castelli,...., Pier Luigi Meroni (2010). European journal of internal medicine. 2010 Feb 01
• Abstract: Thrombolysis with recombinant tissue-type plasminogen activator (rt-PA) is successfully used in acute myocardial infarction with ST elevation (STEMI). Reocclusions follow rt-PA treatment in …

Annexin A2 Is a Molecular Target for TM601, a Peptide with Tumor-targeting and Anti-angiogenic Effects

• https://pubmed.ncbi.nlm.nih.gov/20018898/
• Kamala Kesavan, Judson Ratliff,...., Douglas B Jacoby (2009). pm20018898. 2009 Dec 15
• s were transferred to fresh tubes, snap frozen in liquid nitrogen, and stored at −80 °C. tPA levels and activity were measured using colorimetric enzyme-linked immunosorbent assay kits purchased from AssayPro as per the manufacturer's instructions. The activity is measured as the ability of tPA present in the supernatants to activate plasminogen in an indirect assay that measures the amount of plas

Fish Protein Stimulated the Fibrinolysis in Rats

• https://pubmed.ncbi.nlm.nih.gov/15542926/
• Masakazu Murata, Yoko Sano,...., Motoharu Uchida (2004). Annals of nutrition & metabolism. 2004 Jan 01
• Abstract: We hypothesized that fish protein affects blood coagulation and/or fibrinolysis, and compared the activity and amounts of factors involved in blood coagulation and fibrinolysis in rats fed t…