Human Alpha-1-Antitrypsin Deficient Plasma (Sodium Citrate)

Validating Well-Functioning Hepatic Organoids for Toxicity Evaluation

• https://pubmed.ncbi.nlm.nih.gov/38787150/
• Seo Yoon Choi, Tae Hee Kim,...., William S. Baldwin (2024). Toxics. 2024 May 17
• To quantify the level of alpha-1 antitrypsin (AAT), the culture medium was collected 24 h after replacement with fresh medium and analyzed using a human AAT ELISA quantitation kit (catalog number: EA5101-8; Assaypro, St. Charles, MO, USA), following the manufacturer’s instructions...The absorbance was measured using a microplate reader (S1LFA; BioTek...

Validating Well-Functioning Hepatic Organoids for Toxicity Evaluation

• https://pubmed.ncbi.nlm.nih.gov/38787150/
• Seo Yoon Choi, Tae Hee Kim,...., William S. Baldwin (2024). Toxics. 2024 May 17
• To quantify the level of alpha-1 antitrypsin (AAT), the culture medium was collected 24 h after replacement with fresh medium and analyzed using a human AAT ELISA quantitation kit (catalog number: EA5101-8; Assaypro, St. Charles, MO, USA), following the manufacturer’s instructions...The absorbance was measured using a microplate reader (S1LFA; BioTek...

Patent Culture method for organoids

• No Link Provided
• Meritxell Huch Ortega, Johannes Carolus Clevers, Sylvia Fernandez Boj (2023). us11725184. 2023 Aug 15
• ...injections of 50 mg/animal anti-asialo GM1 (Wako pure chemical industries) to deplete NK cells...To monitor the transplantation state, blood samples were taken in regular intervals from the tail vein and analyzed for the presence of human albumin and human α1-antitrypsin using respective human specific ELISAs (Assaypro)...Karyotyping and Genetic Stability Analysis: Organoid cultures in exponential growing phase were incubated for...

Targeting PRMT5 Activity Inhibits the Malignancy of Hepatocellular Carcinoma by Promoting the Transcription of HNF4α

• https://pubmed.ncbi.nlm.nih.gov/31131056/
• Bai-Nan Zheng, Chen-Hong Ding,...., Wei-Fen Xie (2019). Theranostics. 2019 Jan 01
• Human A1AT was measured by the Human alpha-1-Antitrypsin ELISA Kit (Assay Pro) according to the manufacturer's instructions...For in vitro analysis, HCC cells were treated with 1.0 μM DW14800 in 6-well...

Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver

• https://pubmed.ncbi.nlm.nih.gov/25533785/
• Meritxell Huch, Helmuth Gehart,...., Hans Clevers (2015). Cell. 2015 Jan 15
• ...injections of 50 mg/animal anti-asialo GM1 (Wako Pure Chemical Industries) to deplete NK cells...To monitor the transplantation state, blood samples were taken in regular intervals from the tail vein and were analyzed for the presence of human albumin and human α1-antitrypsin using respective human specific ELISAs (Assaypro).

Proteomic analysis allows for early detection of potential markers of metabolic impairment in very young obese children

• https://pubmed.ncbi.nlm.nih.gov/24949022/
• Gabriel Á Martos-Moreno, Lucila Sackmann-Sala,...., John J Kopchick (2014). International Journal of Pediatric Endocri…. 2014 Jun 10
• The findings in proteomic analysis were contrasted with validated commercial assays by ELISA [Apolipoprotein-A1 (Apo-A1; Assaypro (R) ), haptoglobin (Assaypro (R) ), apo-J/clusterin (BioVendor (R) ), alpha-1B-acid glycoprotein (α-1-BAGP; Assaypro (R) ), alpha-1-antitrypsin (α1-AT; Assaypro (R) ); vitamin D binding protein (vD-BP; R&D Systems (R) ); retinol binding protein 4 (RBP4; R&D Systems (R) )] or kinetic nephelometry [transthyretin (Immage.

Proteomic analysis allows for early detection of potential markers of metabolic impairment in very young obese children

• https://pubmed.ncbi.nlm.nih.gov/24949022/
• Gabriel Á Martos-Moreno, Lucila Sackmann-Sala,...., John J Kopchick (2014). International Journal of Pediatric Endocri…. 2014 Jun 10
• The findings in proteomic analysis were contrasted with validated commercial assays by ELISA [Apolipoprotein-A1 (Apo-A1; Assaypro (R) ), haptoglobin (Assaypro (R) ), apo-J/clusterin (BioVendor (R) ), alpha-1B-acid glycoprotein (α-1-BAGP; Assaypro (R) ), alpha-1-antitrypsin (α1-AT; Assaypro (R) ); vitamin D binding protein (vD-BP; R&D Systems (R) ); retinol binding protein 4 (RBP4; R&D Systems (R) )] or kinetic nephelometry [transthyretin (Immage.

Novel Urinary Protein Biomarkers Predicting the Development of Microalbuminuria and Renal Function Decline in Type 1 Diabetes

• https://pubmed.ncbi.nlm.nih.gov/22238279/
• Daniela Schlatzer, David M. Maahs, Janet K. Snell-Bergeon (2012). Diabetes Care. 2012 Feb 10
• The five biomarkers were measured by commercially available ELISA kits according to the manufacturer’s instructions: Tamms-Horsfall glycoprotein (THP) (MD Biosciences, St. Paul, MN); human progranulin (R & D Systems, Minneapolis, MN); clusterin (BioVendor, Chandler, NC); human α-1 acid glycoprotein (AGP) (Assaypro, St. Charles, MO), and prostaglandin D synthase (ProstD) (BioVendor).

Urinary Glycoprotein Biomarker Discovery for Bladder Cancer Detection using LC-MS/MS and Label-free Quantification

• https://pubmed.ncbi.nlm.nih.gov/21459797/
• Na Yang, Shun Feng,...., Steven Goodison (2011). Clinical cancer research : an official jou…. 2011 May 15
• ...were visualized and quantified using MultiGauge v.3.1 software (FujiFilm Life Science, Inc., Stamford, CT)...The level of human alpha1-antitrypsin (A1AT) was monitored in urine samples using the AssayMax Human Alpha1-Antitrypsin (A1AT) ELISA kit (Assaypro, MO)...The A1AT present in standards and samples is measured by competition with a biotyinylated...