Human Apolipoprotein E (Apo E) ELISA Kit

$407.00

Catalog Number: EA8003-1

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
Product Size:96 Well Plate

Additional information

Format

Sandwich ELISA

Entrez Gene

348

Components

Biotinylated Human Apolipoprotein E Antibody, Chromogen Substrate (1x), EIA Diluent Concentrate (10x), Human Apolipoprotein E Microplate, Human Apolipoprotein E Standard, Sealing Tapes, SP Conjugate (100x), Standard Diluent (1x), Stop Solution (1x), Wash Buffer Concentrate (20x)

TIme

4 hours

Sensitivity

3.5 ng/ml

Range

0.008 – 0.5 ug/ml

Samples Type

CSF, Plasma, Serum

Associated Disease

Alzheimer's Disease, Cancer, Cardiovascular, Heart Disease

Species

Human

UniProt

P02649

Storage

Refer to component labels for details

Plex

1

Usage

For Research Use Only, Not To Be Used For Diagnostic Purposes

Documentation

Publications citing Assaypro: Human Apolipoprotein E (Apo E) ELISA Kit... (31 results)

Please use discretion when following external links; Assaypro cannot guarantee the validity and security of all citation links provided.

Are serum protein biomarkers derived from proteomic analysis useful in screening for trisomy 21 at 11-13 weeks?

  • https://karger.com/fdt/article-abstract/30/1/53/135789
  • AL Mastricci, R Akolekar, R Kuppusamy...- Fetal diagnosis and..., 2011 - karger.com
  • Objective: The aim of this study is to identify potential biomarkers for fetal trisomy 21 from previous publications using proteomic techniques and examine the potential value of such...

Comparison of the effects of endurance and endurance?strength training programmes on the level of endothelial dysfunction in women with abdominal obesity: study protocol for a randomised controlled trial

Genotypes and serum levels of apolipoprotein E and paraoxonase 1 in calcific aortic valve stenosis

Serum amyloid A1 and plasminogen as predictory proteins to monitor the progression of preleukemic diseases towards acute lymphoblastic leukaemia

5D proteomic approach for the biomarker search in plasma: Acute myeloid leukaemia as a case study

Quantitative Proteomics Analysis of Plasmodium vivax Induced Alterations in Human Serum during the Acute and Convalescent Phases of Infection

Clinicopathological Analysis and Multipronged Quantitative Proteomics Reveal Oxidative Stress and Cytoskeletal Proteins as Possible Markers for Severe Vivax Malaria

Serum APOE, leptin, CFH and HTRA1 levels in Pakistani age related macular degeneration patients

Proteomic analysis of Plasmodium falciparum induced alterations in humans from different endemic regions of India to decipher malaria pathogenesis and identify surrogate markers of severity

Gene ploymorphism of lipid metabolism markers in calcific aortic valve disease

Quantitative Proteomic Analysis of Meningiomas for the Identification of Surrogate Protein Markers

Apolipoprotein E downregulates the production of IL-1 in individuals with HIV infection

Gene polymorphism of lipid metabolism markers in calcific aortic valve disease

ANALYSIS OF INDICATORS OF LIPID METABOLIS IN CALCINATING DISEASES OF THE AORTAL VALVE

Pitavastatin increases HDL particles functionally preserved with cholesterol efflux capacity and antioxidative actions in dyslipidemic patients

Central leptin and insulin administration modulates serum cytokine-and lipoprotein-related markers

Are Serum Protein Biomarkers Derived from Proteomic Analysis Useful in Screening for Trisomy 21 at 11-13 Weeks?

APOE e4 allele and CSF APOE on Cognition in HIV-Infected Subjects

Comparison of the Effect of Amaranth Oil vs. Rapeseed Oil on Selected Atherosclerosis Markers in Overweight and Obese Subjects: A Randomized Double-Blind Cross-Over Trial

  • https://pubmed.ncbi.nlm.nih.gov/34444289/
  • MałgorzataMałgorzata Jamka Jamka, AnnaAnna Morawska Morawska (2021). International Journal of Environmental Res…. 2021 Aug 12
  • .. using standard methods and stored at −70 °C until analysis.. The following parameters were measured using the enzyme-linked immunosorbent assay methods: TNF-α (DRG Instruments GmbH, Marburg, Germany), adiponectin (Mediagnost, Reutlingen, Germany), ox-LDL (Shanghai Sunred Biological Technology, Shanghai, China) and ApoE (Assay Max Human Lipoprotein E, Assaypro, St. Charles, MO, USA).. ApoA1 and ApoB levels were assessed by an immunonephelometric method ..

Preclinical atherosclerosis in cystic fibrosis: Two distinct presentations are related to pancreatic status

  • https://pubmed.ncbi.nlm.nih.gov/34253491/
  • Jan Krzysztof Nowak, Andrzej Wykrętowicz, ..., Jarosław Walkowiak (2021). Journal of Cystic Fibrosis. 2021 Jul 01
  • Abstract: Patients with cystic fibrosis (CF) are exposed to overlapping cardiovascular risk factors. We hypothesized that CF is characterized by increased arterial stiffness and greater intima-media t…

Cyclodextrin-micellar electrokinetic chromatography of apolipoproteins on human very low-density lipoprotein.

  • https://pubmed.ncbi.nlm.nih.gov/32390137/
  • Ying-Tzu Shieh, Chiz-Tzung Chang, ..., Mine-Yine Liu (2021). Electrophoresis. 2021 Mar 22
  • In this study, the Human Apolipoprotein C III Apo C3 AssayMax ELISA Kit from Assaypro was employed to quantify Apo CIII concentrations in VLDL samples. This measurement was crucial for…

Cystic fibrosis dyslipidaemia: A cross-sectional study.

  • https://pubmed.ncbi.nlm.nih.gov/30979683/
  • Jan Krzysztof Nowak, Mariusz Szczepanik, ..., Jarosław Walkowiak (2020). Journal of cystic fibrosis : official jour…. 2020 Sep 17
  • .. mmol/L).Hypocholesterolaemia was defined based on the local laboratory reference ranges (b115 mg/dL; b3.0 mmol/L).. The following ELISA kits were used: human oxLDL (Shanghai Sunred Biological Technology Co., Shanghai, China), human adiponectin (Mediagnost, Reutlingen, Germany), and AssayMax Human Apolipoprotein E (Assaypro, St. Charles, USA).. In a subset of patients for whom sufficient samples were available, serum TNF-α was ..

Apolipoprotein E binds to and reduces serum levels of DNA-mimicking, pyrrolated proteins

  • https://pubmed.ncbi.nlm.nih.gov/31167785/
  • Sayumi Hirose, Yusuke Hioki, ..., Koji Uchida (2019). Journal of Biological Chemistry. 2019 Jul 01
  • .. from Sigma.Purified thrombin from human plasma and dsDNA, and PLL were obtained from Sigma.. Serum apoE was measured using the human ApoE ELISA kit (Assaypro).. DAPI (4',6diamidino-2-phenylindole) was obtained from Dojindo (Kumamoto, Japan).DAPI (4',6diamidino-2-phenylindole) was obtained from Dojindo (Kumamoto, ..

5D proteomic approach for the biomarker search in plasma: Acute myeloid leukaemia as a case study

  • https://pubmed.ncbi.nlm.nih.gov/29180721/
  • Syed Kashif Raza, Mahwish Saleem, Syed Ghulam Musharraf (2017). Scientific Reports. 2017 Nov 27
  • The substrate solution was then added and left for 10 min for reaction completion.. This procedure with some modifications according to the manufacturer’s protocol was applied to validate our results for selected proteins; complement factor C7, plasminogen, and ApoE, for which ELISA kits were purchased from Assaypro (USA).. Statistics and data analysis The analysis of 2-DGE images, detection of spots, spot matching, ..

Quantitative Proteomics Analysis of Plasmodium vivax Induced Alterations in Human Serum during the Acute and Convalescent Phases of Infection

  • https://pubmed.ncbi.nlm.nih.gov/28667326/
  • Sandipan Ray, Sandip K Patel, ..., Sanjeeva Srivastava (2017). Scientific Reports. 2017 Jun 30
  • , Serum amyloid A (SAA; P0DJI8), Hemopexin (HPX; P02790), Apolipoprotein E (Apo E; P02649), Haptoglobin (HP; P00738), and Apolipoprotein A1 (Apo A1; P02647) were quantified using AssayMax ELISA kits (AssayPro, USA) in serum samples from LPVM, MPVM and DF patients, and healthy controls following the manufacturer’s instructions. Serum abundances of these five proteins along with three other proteins

Proteomic Profile of Unstable Atheroma Plaque: Increased Neutrophil Defensin 1, Clusterin, and Apolipoprotein E Levels in Carotid Secretome

  • https://pubmed.ncbi.nlm.nih.gov/26795031/
  • Gemma Aragonès, Teresa Auguet, ..., Cristóbal Richart (2016). Journal of Proteome Research. 2016 Feb 03
  • Abstract: Because of the clinical significance of carotid atherosclerosis, the search for novel biomarkers has become a priority. The aim of the present study was to compare the protein secretion prof…

Quantitative Proteomic Analysis of Meningiomas for the Identification of Surrogate Protein Markers

  • https://pubmed.ncbi.nlm.nih.gov/25413266/
  • Samridhi Sharma, Sandipan Ray, ..., Sanjeeva Srivastava (2014). Scientific Reports. 2014 Nov 21
  • ferent grades of meningiomas; grade I (n = 14), grade II (n = 5) and grade III (n = 1) and healthy controls (n = 45) were measured using AssayMax ELISA kits following the manufacturer's instructions (AssayPro, USA). Optical densities were measured at 450 nm and 570 nm using a SpectraMax M2e (Molecular Devices, USA). Statistical significance of differential expression of the serum proteins was anal

Serum Protein Signatures Differentiating Autoimmune Pancreatitis versus Pancreatic Cancer

  • https://pubmed.ncbi.nlm.nih.gov/24349355/
  • Klaus Felix, Oliver Hauck, ..., Jens Werner (2013). PLoS ONE. 2013 Dec 09
  • phase capture sandwich ELISAs were performed using commercially available kits for apolipoprotein A-I (ALerCHEK Inc., Portland, Maine). Apolipoprotein A-II, apolipoprotein E and haptoglobin were from AssayPro (AssayPro, St. Charles, MO), tetranectin from USCN Life Science Inc. (Wuhan, PRC), and transthyretin from Immundiagnostik AG (Bensheim, Germany). The haptoglobin ELISA from AssayPro is also a

Pitavastatin Increases HDL Particles Functionally Preserved with Cholesterol Efflux Capacity and Antioxidative Actions in Dyslipidemic Patients

  • https://pubmed.ncbi.nlm.nih.gov/23739642/
  • Maki Miyamoto-Sasaki, Tomoyuki Yasuda, ..., Ken-ichi Hirata (2013). Journal of atherosclerosis and thrombosis. 2013 Jan 01
  • All values are presented as the mean±SD. Comparisons between the parameters of pre- and postpitavastatin treatment were made with paired Stu- measured using a commercially available ELISA kit (ASSAYPRO, MO, USA). The lipoprotein profiles were determined using HPLC (LipoSEARCH®) at Skylight Biotech, Inc. (Akita, Japan), according to the specified procedure21). The levels of HDL-phospholipids, total

Central leptin and insulin administration modulates serum cytokine- and lipoprotein-related markers

  • https://pubmed.ncbi.nlm.nih.gov/22658937/
  • Emma Burgos-Ramos, Lucila Sackmann-Sala, ..., John J Kopchick (2012). Metabolism. 2012 Nov 01
  • Abstract: In most obese patients there is an inflammatory state characterized by lipid abnormalities, hyperleptinemia and hyperinsulinemia. The objective was to identify mechanisms involved in leptin'…

Effect of apoA-I on cholesterol release and apoE secretion in human mature adipocytes

  • https://pubmed.ncbi.nlm.nih.gov/20642861/
  • Karima Bencharif, Laurence Hoareau, ..., Régis Roche (2010). Lipids in health and disease. 2010 Jul 20
  • .. for 12 hours followed by BFA and with or not apoA-I for 6 hours.. Culture medium samples were collected and assayed for apoE with The AssayMax Human ApoE ELISA (Assaypro LLC, USA) according to the manufacturer’s instructions.. RNA extraction, reverse transcription and real-time quantitative PCR Cells from 6 wells (3 × ..

Loading data...