Career Opportunities

Protein Purification Scientist

Position Summary

We are seeking an experienced and highly motivated Protein Purification Scientist to join our research and development team. The Scientist will be responsible for the purification, characterization, and optimization of native or recombinant proteins and other biologics using FPLC and advanced chromatography techniques.

The ideal candidate will have strong hands-on experience with ÄKTA/FPLC systems or other similar system, protein chromatography, process optimization, and protein characterization. The individual should be capable of independently designing and executing experiments, troubleshooting purification processes, analyzing data, and communicating results effectively.

Key Responsibilities

• Design, develop, and execute protein purification strategies for native or recombinant proteins, antibodies, and other biologic molecules.
• Perform protein purification using FPLC/ÄKTA systems or similar systems and optimize chromatography conditions.
• Develop and optimize purification processes using:
  • Affinity chromatography
  • Ion-exchange chromatography
  • Size-exclusion chromatography
  • Hydrophobic interaction chromatography
• Develop purification workflows from crude biological material through highly purified protein.
• Optimize purification parameters including buffer composition, pH, conductivity, salt concentration, flow rate, loading capacity, and gradient conditions.
• Analyze FPLC chromatograms and purification data to evaluate protein yield, purity, and recovery.
• Perform protein concentration, buffer exchange, and formulation using ultrafiltration/diafiltration and dialysis.
• Characterize purified proteins using SDS-PAGE, Western blot, UV absorbance, and other appropriate analytical techniques.
• Troubleshoot protein purification challenges including low recovery, aggregation, precipitation, poor resolution, and column performance.
• Develop, document, and improve laboratory protocols and standard operating procedures.
• Maintain accurate experimental records and prepare technical reports and presentations.
• Independently plan experiments, interpret results, and make recommendations for process improvement.
• Collaborate with assay development, and analytical teams.
• Support technology transfer, process scale-up, and development of robust purification processes as needed.
• Maintain laboratory equipment and ensure compliance with laboratory safety and quality procedures.

Required Qualifications

• MS or PhD in Biochemistry, Biotechnology, Molecular Biology, Chemistry, or a related scientific discipline.
• 3+ years of hands-on experience in protein purification, preferably in an industry or biotechnology research environment.
• Demonstrated expertise with FPLC/ÄKTA chromatography systems or similar systems.
• Strong practical experience with affinity, ion-exchange, and size-exclusion chromatography.
• Experience with plasma protein purification and protein characterization.
• Strong understanding of protein chemistry, stability, aggregation, and purification principles.
• Experience analyzing chromatograms and interpreting purification data.
• Ability to independently design and execute experiments and troubleshoot technical problems.
• Strong scientific writing, documentation, communication, and data-analysis skills.

Preferred Qualifications

• PhD in Biochemistry, Biotechnology, Protein Chemistry, or a related field.
• Experience with human plasma or serum and other biological fluid protein purification.
• Experience with protein formulation and stability studies.
• Experience developing purification processes suitable for scale-up.
• Familiarity with GMP/GLP environments and quality systems.
• Experience developing SOPs and technical reports.

Core Competencies

• Protein purification and chromatography
• FPLC/ÄKTA operation and method development
• Protein chemistry and characterization
• Experimental design and troubleshooting
• Data analysis and interpretation
• Scientific documentation
• Cross-functional collaboration
• Independent problem solving
• Attention to detail

Success in This Role

The successful candidate will be able to independently develop and optimize protein purification processes, operate FPLC systems effectively, troubleshoot purification challenges, and deliver high-quality purified proteins with reproducible yield and purity.

Lab Technician - One Full Time Position

Duties Responsibilities

• Manufacturing and testing immunoassay kits, antibodies, activity kits, and proteins.
• Weighing solids and being detail oriented.
• Evaluating raw materials for use in production.
• Maintaining records of results and status on all finished products and raw materials.
• Using Microsoft Word, Excel, Access, PowerPoint, Outlook and SoftMax Pro.
• Ensuring operation of equipment by completing preventive maintenance requirements; evaluating new equipment and techniques.
• Calibrating test equipment and maintaining calibration documentation.
• Maintaining lab-established practices, including sample retention and disposal in accordance with established procedures.
• Assisting the organization to create a safe and healthy working environment by working safely, using equipment provided and following instructions given for health and safety purposes, and immediately reporting any unsafe working practices or hazardous working conditions.
• Assisting in the assembling, packaging, and shipping of products to customers.
• Maintaining supplies inventory by checking stock to determine inventory level; anticipating needed supplies; verifying receipt of supplies.

Qualifications Requirements:

• BS/MS with major in biology, chemistry, or biotechnology/biochemistry preferred
• Must be detail-oriented and organized

Job Code

2302