Human alpha-1-Antitrypsin (A1AT) ELISA Kit

Long-term expansion of functional mouse and human hepatocytes as 3D organoids

• https://www.cell.com/cell/fulltext/S0092-8674(18)31505-8
• H Hu, H Gehart, B Artegiani, C Lopez-Iglesias...- Cell, 2018 - cell.com
• The mammalian liver possesses a remarkable regenerative ability. Two modes of damage response have been described: (1) The oval cell response emanates from the biliary tree...

SARS-CoV-2 infection results in upregulation of Plasminogen Activator Inhibitor-1 and Neuroserpin in the lungs, and an increase in fibrinolysis inhibitors associated...

• https://onlinelibrary.wiley.com/doi/abs/10.1002/jha2.654
• KH Toomer, GF Gerber, Y Zhang, L Daou, M Tushek...-..., 2023 - Wiley Online Library
• Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection results in coagulation activation although it is usually not associated with consumption coagulopathy...

Novel hepatocyte-like liver organoids recapitulate crucial mature hepatic functions

• https://www.biorxiv.org/content/10.1101/2024.10.29.620824.abstract
• AI Ardisasmita, IP Joore, N Levy, A Myszczyszyn...- bioRxiv, 2024 - biorxiv.org
• Accurate liver disease modeling and drug toxicity testing still remain challenging as liver cells in vitro poorly resemble adult hepatocytes, as we previously demonstrated using whole...

Role of oxidative stress and outcome of various surgical approaches among patients with bullous lung disease candidate for surgical interference

• https://pmc.ncbi.nlm.nih.gov/articles/PMC5107534/
• A Farouk, MH Hassan, MA Nady...- Journal of Thoracic..., 2016 - pmc.ncbi.nlm.nih.gov
• Background Bullous lung disease is characterized by formation of blebs, bullae and emphysema. We investigate the role of oxidative stress in the pathogenesis of bullous lung disease...

Validating Well-Functioning Hepatic Organoids for Toxicity Evaluation

• https://www.mdpi.com/2305-6304/12/5/371
• SY Choi, TH Kim, MJ Kim, SJ Mun, TS Kim, KK Jung...- Toxics, 2024 - mdpi.com
• Organoids, three-dimensional self-organized organ-like miniature tissues, are proposed as intermediary models that bridge the gap between animal and human studies in drug...

Measuring serum prothrombin in patients treated with warfarin or direct oral anticoagulants

• https://pubmed.ncbi.nlm.nih.gov/34599901/#affiliation-1
• Kondo A et al. (2021). Clin Chim Acta. 523:224-230.
• No Snippet Provided

Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media

• https://www.nature.com/articles/s41598-020-73908-1.pdf
• Sekine K et al. (2020). 10(1):17937.
• No Snippet Provided

Large-Scale Production of LGR5-Positive Bipotential Human Liver Stem Cells

• https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496924/pdf/HEP-72-257.pdf
• Schneeberger K et al. (2020). Hepatology. 72(1):257-270
• No Snippet Provided

Enhanced secretion of human alpha1-antitrypsin expressed with a novel glycosylation module in tobacco BY-2 cell culture

• https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527068/pdf/kbie-10-01-1604037.pdf
• Zhang N et al. (2019). Bioengineered 10(1):87-97.
• No Snippet Provided

Long-Term Expansion of Functional Mouse and Human Hepatocytes as 3D Organoids

• https://www.sciencedirect.com/science/article/pii/S0092867418315058.
• Hu H et al. (2018). Cell. 175(6):1591-1606.e19.
• No Snippet Provided

Artificial neural network-based serum biomarkers analysis improves sensitivity in the diagnosis of lung cancer

• https://www.researchgate.net/profile/Paula-Cabrera-3/publication/289389786_Artificial_Neural_Network-Based_Serum_Biomarkers_Analysis_Improves_Sensitivity_in_the_Diagnosis_of_Lung_Cancer/links/5ecc8ed4a6fdcc90d699a18a/Artificial-Neural-Network-Based-Serum-Biomarkers-Analysis-Improves-Sensitivity-in-the-Diagnosis-of-Lung-Cancer.pdf
• Flores JM (2013). IFMBE Proceedings. 33:882-885.
• No Snippet Provided

Activity-dependent neuroprotector homeobox protein: A candidate protein identified in serum as diagnostic biomarker for Alzheimer's disease

• https://www.researchgate.net/profile/Yu-Chang-Tyan/publication/224896256_Activity-dependent_neuroprotector_homeobox_protein_A_candidate_protein_identified_in_serum_as_diagnostic_biomarker_for_Alzheimer's_disease/links/59fb05c0a6fdcca1f28fa9b4/Activity-dependent-neuroprotector-homeobox-protein-A-candidate-protein-identified-in-serum-as-diagnostic-biomarker-for-Alzheimers-disease.pdf.
• Yang MH et al. (2012). J Proteomics. 75(12):3617-29.
• No Snippet Provided

alpha1-Antitrypsin low-density-lipoprotein serves as a marker of smoking-specific oxidative stress

• https://www.jstage.jst.go.jp/article/jat/advpub/0/advpub_9035/_pdf
• Wada H et al. (2011). J Atheroscler Thromb. 19(1):47-58.
• No Snippet Provided

Effects of Chronic Rosiglitazone Treatment on Renal Handling of Salt and Water in Rats With Volume-Overload Congestive Heart Failure

• https://pdfs.semanticscholar.org/9eb5/6e7db3e7617488c427027b96f8052023c3f9.pdf
• Goltsman I et al. (2011). Circ Heart Fail. 4(3):345-54
• No Snippet Provided

Local regulation of neutrophil elastase activity by endogenous alpha1-antitrypsin in lipopolysaccharide-primed hematological cells

• https://pubmed.ncbi.nlm.nih.gov/21624645/
• Dokai M et al. (2011). Thrombosis Research 128(3):283-292
• No Snippet Provided

A human liver organoid platform for hepatotoxicity assessment: evaluation using reference compounds

• https://pubmed.ncbi.nlm.nih.gov/42371511/
• Min Jeong Kim, Seo Yoon Choi, ..., Jin Hee Lee (2026). Frontiers in Toxicology. 2026 Jun 15
• .. replacement.To measure α1-antitrypsin (AAT) secretion, culture supernatants were collected 24 h after medium replacement.. Quantification was performed using a human AAT ELISA kit (Catalog No. EA5101-8; AssayPro, St. Charles, MO, United States) according to the manufacturer’s instructions.. Absorbance was recorded using a microplate reader (S1LFA; BioTek Instruments, Inc.), and the values ..

Validating Well-Functioning Hepatic Organoids for Toxicity Evaluation

• https://pubmed.ncbi.nlm.nih.gov/38787150/
• Seo Yoon Choi, Tae Hee Kim, ..., William S. Baldwin (2024). Toxics. 2024 May 17
• To quantify the level of alpha-1 antitrypsin (AAT), the culture medium was collected 24 h after replacement with fresh medium and analyzed using a human AAT ELISA quantitation kit (catalog number: EA5101-8; Assaypro, St. Charles, MO, USA), following the manufacturer’s instructions.. The absorbance was measured using a microplate reader (S1LFA; BioTek ..

SARS-CoV-2 infection results in upregulation of Plasminogen Activator Inhibitor-1 and Neuroserpin in the lungs, and an increase in fibrinolysis inhibitors associated with disease severity.

• https://pubmed.ncbi.nlm.nih.gov/37206290/
• Kevin H Toomer, Gloria F Gerber, ..., Ivo M B Francischetti (2023). EJHaem. 2023 May 01
• ELISA for Antithrombin (AT, SERPINC1, EA3301-1), Thrombin-antithrombin complex (TAT; ET1020-1),α-2-Antiplasmin (A2AP, SERPINF2, EA34771), Plasmin-α2-Antiplasmin Complex (PAP; EP1807-1), Tissue Plasminogen Activator/Plasminogen Activator Inhibitor-1 complex (tPA/PAI-1; EP1105-1), Factor XIII (FXIII; EF1013-1), α-1-Antitrypsin (A1AT, SERPINA1; EA5101-1), Protein Z (PZ; EP3333-1), Protein C (PC, EP2312-1), Protein S (total) (PS, EP133-1), and Vaspin (SERPIN12; EV3005-1) from Assaypro, LLC (St. Charles, MO).

Generation of human induced pluripotent stem cell-derived liver buds with chemically defined and animal origin-free media

• https://pubmed.ncbi.nlm.nih.gov/33087763/
• Keisuke Sekine, Shimpei Ogawa, ..., Hideki Taniguchi (2020). Scientific Reports. 2020 Oct 21
• ed at 3000g for 10 min to collect serum. Human albumin levels in the medium and mouse serum samples were measured using the human albumin ELISA kit (Bethyl Laboratories). Human AAT levels in mouse serum samples were measured using the human AAT AssayMax ELISA kit (AssayPro), according to the manufacturer’s instructions. Adult human hepatocytes were purchased from manufacturers (dBioIVT, Kurabo).

Targeting PRMT5 Activity Inhibits the Malignancy of Hepatocellular Carcinoma by Promoting the Transcription of HNF4α.

• https://pubmed.ncbi.nlm.nih.gov/31131056/
• Bai-Nan Zheng, Chen-Hong Ding, ..., Wei-Fen Xie (2020). Theranostics. 2020 May 07
• Human A1AT was measured by the Human alpha-1-Antitrypsin ELISA Kit (Assay Pro) according to the manufacturer’s instructions.. For in vitro analysis, HCC cells were treated with 1.0 μM DW14800 in 6-well ..

Large‐Scale Production of LGR5‐Positive Bipotential Human Liver Stem Cells

• https://pubmed.ncbi.nlm.nih.gov/31715015/
• KerstinKerstinKerstin Schneeberger Schneeberger Schneeberger, NataliaNatalia Sánchez‐Romero Sánchez‐Romero (2020). Hepatology (Baltimore, Md.). 2020 Apr 08
• GLDH was measured in the lysates using a DxC‐600 Beckman chemistry analyzer (Beckman Coulter).. A1AT was measured using an AssayMax A1AT enzyme‐linked immunosorbent assay kit (AssayPro, St. Charles, MO).. Values were normalized to live cell numbers.

Long-Term Expansion of Functional Mouse and Human Hepatocytes as 3D Organoids.

• https://pubmed.ncbi.nlm.nih.gov/30500538/
• Huili Hu, Helmuth Gehart, ..., Hans Clevers (2019). Cell. 2019 Aug 27
• .. R&D 295OM DAPI Thermo D1306 Critical Commercial Assays Di-Ac-Ldl Biomedical technologogies BT-902 Rhodamine 123 Thermo R302 Cytochrome activity kit CYP3A4 Assay V8901 Mouse Alb Elisa kit Bethyl E99-134 Human ALB Elisa kit Bethyl E88-129 Human a1-Antitrypsin Elisa kit Assaypro EA5101-1 Human alpha-Fetoprotein ELISA Kit R&D systems DAFP00 CellTiter-Glo 3D Cell Viability Assay Promega G9682 Thermo Scientific reagents for CEL-Seq2 Hashimshony et al., 2016 N/A Deposited Data Raw and analyzed sequencing and microarray data This paper ..

Enhanced secretion of human α1-antitrypsin expressed with a novel glycosylation module in tobacco BY-2 cell culture

• https://pubmed.ncbi.nlm.nih.gov/30957636/
• Ningning Zhang, Tristen Wright, ..., Jianfeng Xu (2019). Bioengineered. 2019 Jan 01
• Abstract: Expression of recombinant proteins fused to a novel glycomodule tag, termed hydroxyproline (Hyp)-O-glycosylated peptides (HypGP), was earlier found to boost secreted protein yields up to 500…

Reduced levels of alpha-1-antitrypsin in cerebrospinal fluid of amyotrophic lateral sclerosis patients: a novel approach for a potential treatment

• https://pubmed.ncbi.nlm.nih.gov/27245439/
• Uri Wormser, Jessica Mandrioli, ..., Yoram Finkelstein (2016). Journal of neuroinflammation. 2016 Jun 01
• AAT was determined (monoplicate) by ELISA kit validated for CSF (Assaypro, St. Charles, MO, USA, Cat No. EA5101-1) and cytokines by Human Magnetic Luminex screening assay (R&D Systems, Minneapolis, MN, USA).

Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver

• https://pubmed.ncbi.nlm.nih.gov/25533785/
• Meritxell Huch, Helmuth Gehart, ..., Hans Clevers (2015). Cell. 2015 Jan 15
• .. same medium without growth factors and were used as positive and negative control respectively.. The amount of albumin and A1AT in culture supernatant was determined using a human specific Albumin or human specific A1AT ELISA kit (both from Assay Pro).. To measure Cyp3a activity the cultures were differentiated as described and the day of ..

Activity-dependent neuroprotector homeobox protein: A candidate protein identified in serum as diagnostic biomarker for Alzheimer's disease

• https://pubmed.ncbi.nlm.nih.gov/22554909/
• Ming-Hui Yang, Yuan-Han Yang, ..., Yu-Chang Tyan (2012). Journal of proteomics. 2012 Jun 01
• Abstract: Alzheimer's disease (AD) is the most common cause of dementia of late life. To enhance our understanding of AD proteome, the serum proteins were analyzed using two-dimensional gel electropho…

α1-Antitrypsin Low-Density-Lipoprotein Serves as a Marker of Smoking-Specific Oxidative Stress

• https://pubmed.ncbi.nlm.nih.gov/22027559/
• Hiromichi Wada, Shuichi Ura, ..., Koji Hasegawa (2012). Journal of atherosclerosis and thrombosis. 2012 Jan 01
• manufacturer’s instructions (Quantikine; R Systems, Minneapolis, MN, USA, for leptin, VEGF, sVEGFR-1, and sVEGFR-2; CycLex, MBL, Nagano, Japan for adiponectin and hsCRP; Invitrogen, CA, USA for SAA; Assaypro, MO, USA for AT). The sensitivities of the assays for VEGF and sVEGFR-1 were 5.0 and 3.5 pg/mL, respectively. Serum was diluted 5-fold for measurement of the sVEGFR-2 concentration, and the mi

Local regulation of neutrophil elastase activity by endogenous α1-antitrypsin in lipopolysaccharide-primed hematological cells

• https://pubmed.ncbi.nlm.nih.gov/21624645/
• Momoko Dokai, Seiji Madoiwa, ..., Yoichi Sakata (2011). Thrombosis research. 2011 Sep 01
• Abstract: Neutrophil elastase released from activated neutrophils contributes in combating bacterial infection. While chronic inflammation results in anemia and decreased bone marrow activities, littl…

Effects of Chronic Rosiglitazone Treatment on Renal Handling of Salt and Water in Rats With Volume-Overload Congestive Heart Failure

• https://pubmed.ncbi.nlm.nih.gov/21398416/
• Ilia Goltsman, Xinkang Wang, ..., Joseph Winaver (2011). Circulation: Heart Failure. 2011 May 01
• Abstract: The side effects of fluid retention and edema of the thiazolidinedione (TZD) class of peroxisome proliferator-activated receptor-γ agonists limit their use in patients with congestive heart …

Urinary Glycoprotein Biomarker Discovery for Bladder Cancer Detection Using LC/MS-MS and Label-Free Quantification

• https://pubmed.ncbi.nlm.nih.gov/21459797/
• Na Yang, Shun Feng, ..., Steven Goodison (2011). Clinical Cancer Research. 2011 Apr 01
• The level of human A1AT was monitored in urine samples by using the AssayMax Human A1AT ELISA kit (Assaypro).. The A1AT present in standards and samples is measured by competition with a biotinylated ..

Comparison of Hepatic-like Cell Production from Human Embryonic Stem Cells and Adult Liver Progenitor Cells: CAR Transduction Activates a Battery of Detoxification Genes

• https://pubmed.ncbi.nlm.nih.gov/21210253/
• Natalie Funakoshi, Cedric Duret, ..., Sabine Gerbal-Chaloin (2011). Stem Cell Reviews. 2011 Jan 06
• ELISA Albumin and AAT proteins were quantified in 4 day-aliquots of culture media using AssayPro ELISA Kits (St Charles, MO) as recommended.