Human Antithrombin III (AT3) ELISA Kit

$407.00

Catalog Number: EA3301-1

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
Product Size:96 Well Plate

Catalog Number. EA3301-1 Categories: , , Tags: , , , , Brand:

Additional information

Format

Sandwich ELISA

Entrez Gene

462

Components

Biotinylated Human Antithrombin III Antibody, Chromogen Substrate (1x), Human Antithrombin III Microplate, Human Antithrombin III Standard, MIX Diluent Concentrate (10x), Sealing Tapes, SP Conjugate (100x), Stop Solution (1x), Wash Buffer Concentrate (20x)

TIme

4 hours

Sensitivity

0.99 ng/ml

Range

1.563 – 100 ng/ml; 0.010 – 0.67 mIU/ml

Samples Type

CSF, Milk, Plasma, Saliva, Serum, Urine

Associated Disease

Cardiovascular

Species

Human

UniProt

P01008

Storage

Refer to component labels for details

Plex

1

Usage

For Research Use Only, Not To Be Used For Diagnostic Purposes

Documentation

Publications citing Assaypro: Human Antithrombin III (AT3) ELISA Kit... (31 results)

Please use discretion when following external links; Assaypro cannot guarantee the validity and security of all citation links provided.

Coagulation failure is associated with bleeding events and clinical outcome during systemic inflammatory response and sepsis in acute-on-chronic liver failure: an...

  • https://onlinelibrary.wiley.com/doi/abs/10.1111/liv.14034
  • M Premkumar, P Saxena, D Rangegowda...- Liver..., 2019 - Wiley Online Library
  • Background Patients with acute-on-chronic liver failure (ACLF) have coagulation failure in the setting of systemic inflammatory syndrome (SIRS), sepsis and extra-hepatic organ failures

SARS-CoV-2 infection results in upregulation of Plasminogen Activator Inhibitor-1 and Neuroserpin in the lungs, and an increase in fibrinolysis inhibitors associated...

  • https://onlinelibrary.wiley.com/doi/abs/10.1002/jha2.654
  • KH Toomer, GF Gerber, Y Zhang, L Daou, M Tushek...-..., 2023 - Wiley Online Library
  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection results in coagulation activation although it is usually not associated with consumption coagulopathy...

An Investigative Study of Prothrombin Time, Activated Partial Thromboplastin Time and Antithrombin Levels in Patients with Ischaemic Stroke

  • http://insights.sent2promo.com/id/eprint/2959/
  • OC Nnachi, IP Ezenwenyi...-... Blood Research &..., 2024 - insights.sent2promo.com
  • Background: The haemostatic system maintains a balance between prothrombotic and antithrombotic components in the body. The role of this system in ischaemic stroke is not certain....

Heparin-like effect associated with risk of bleeding, sepsis, and death in patients with severe alcohol-associated hepatitis

Indicators of Endothelial dysfunction and factors hemostasis in patients with stable and unstable atherosclerotic plaques. Scientific Research Institute of therapy and preventive medicine.

Indicator of endothelial dysfunction and factors hemostasis in patients with stable and unstable atherosclerotic plates

Measuring serum prothrombin in patients treated with warfarin or direct oral anticoagulants

Cystathionine ?-synthase deficiency: different changes in proteomes of thrombosis-resistant Cbs?/? mice and thrombosis-prone CBS?/? humans

Antithrombin Levels in Adults with Ischaemic Stroke seen at the University of Benin Teaching Hospital, Benin City

Astaxanthin attenuated thrombotic risk factors in type 2 diabetic patients. Journal of Functional Foods. 53:22-27

Antithrombin Debrecen (p. Leu205Pro)?Clinical and molecular characterization of a novel mutation associated with severe thrombotic tendency

Markers of Inflammation and Thrombophilia in Psoriasis

SARS-CoV-2 infection results in upregulation of Plasminogen Activator Inhibitor-1 and Neuroserpin in the lungs, and an increase in fibrinolysis inhibitors associated with disease severity.

  • https://pubmed.ncbi.nlm.nih.gov/37206290/
  • Kevin H Toomer, Gloria F Gerber, ..., Ivo M B Francischetti (2023). EJHaem. 2023 May 01
  • ELISA for Antithrombin (AT, SERPINC1, EA3301-1), Thrombin-antithrombin complex (TAT; ET1020-1),α-2-Antiplasmin (A2AP, SERPINF2, EA34771), Plasmin-α2-Antiplasmin Complex (PAP; EP1807-1), Tissue Plasminogen Activator/Plasminogen Activator Inhibitor-1 complex (tPA/PAI-1; EP1105-1), Factor XIII (FXIII; EF1013-1), α-1-Antitrypsin (A1AT, SERPINA1; EA5101-1), Protein Z (PZ; EP3333-1), Protein C (PC, EP2312-1), Protein S (total) (PS, EP133-1), and Vaspin (SERPIN12; EV3005-1) from Assaypro, LLC (St. Charles, MO).

Heparin-like Effect Associated With Risk of Bleeding, Sepsis, and Death in Patients With Severe Alcohol-Associated Hepatitis.

  • https://pubmed.ncbi.nlm.nih.gov/31077821/
  • Madhumita Premkumar, Chhagan Bihari, ..., Shiv Kumar Sarin (2021). Clinical gastroenterology and hepatology :…. 2021 Aug 18
  • In the study, the Human Antithrombin III AT3 AssayMax ELISA Kit from Assaypro was crucial for measuring antithrombin III levels, which helped elucidate the heparin-like effect in patie…

Cystathionine β-synthase deficiency: different changes in proteomes of thrombosis-resistant Cbs−/− mice and thrombosis-prone CBS−/− humans

  • https://pubmed.ncbi.nlm.nih.gov/32612202/
  • Marta Sikora, Izabela Lewandowska, ..., Hieronim Jakubowski (2020). Scientific Reports. 2020 Jul 01
  • ELISA and western blot assays To validate differentially expressed proteins, we quantified human SERPINC1 and GPX3, mouse Gpx3, Pon1, and Serpinc1 using commercial ELISA kits (FineBiotech and AssayPro). Triplicate assays were performed following the manufacturers’ protocols. A450 was read using microplate reader (Infinite M200 Pro, Tecan, Switzerland). Mouse Mup20 and IgG were quantified by Wester

Coagulation failure is associated with bleeding events and clinical outcome during systemic inflammatory response and sepsis in acute-on-chronic liver failure: An observational cohort study.

  • https://pubmed.ncbi.nlm.nih.gov/30589495/
  • Madhumita Premkumar, Priyanka Saxena, ..., Shiv K Sarin (2020). Liver international : official journal of …. 2020 May 12
  • In the observational cohort study on patients with acute-on-chronic liver failure, the Human Antithrombin III AT3 AssayMax ELISA Kit from Assaypro was employed to evaluate antithrombin…

Association of some hemostasis and endothelial dysfunction factors with probability of presence of vulnerable atherosclerotic plaques in patients with coronary atherosclerosis

  • https://pubmed.ncbi.nlm.nih.gov/31196144/
  • Yu I Ragino, E V Striukova, ..., E V Kashtanova (2019). BMC research notes. 2019 Jun 13
  • ampling from a vein to obtain plasma and serum. Following hemostasis factors: factor II, factor VII, factor XII, and antithrombin III in the blood plasma were determined by ELISAs method (test system AssayPro). Following endothelial dysfunction factors: endothelin 1 (Biomedica), MCP-1 (Bender Medsystems), sVCAM-1 (Biosource), ADMA (Immunodiagnost), homocysteine (Ahis-Shield), and PAI-1 (Technoclone

Systemic Hyperfibrinolysis after Trauma: A Pilot study of Targeted Proteomic Analysis of Superposed Mechanisms in Patient Plasma

  • https://pubmed.ncbi.nlm.nih.gov/29554044/
  • Anirban Banerjee, Christopher C Silliman, ..., Kirk Hansen (2019). The journal of trauma and acute care surge…. 2019 Jun 01
  • quantification, per the manufacturer’s instructions. ELISA’s were completed for thrombin (MyBiosource.com, San Diego, CA), antithrombin (AT), thrombin:antithrombin (TAT) complexes, plasminogen (Plg) (AssayPro, St. Charles, MO), α2 -antiplasmin, plasmin: α2 -antiplasmin (PAP) complexes, tPA, tPA:PAI-1 complexes and PAI-1 (Molecular Innovations, Novi, MI), and thrombin-activated fibrinolysis inhibito

Antithrombin Debrecen (p.Leu205Pro) - Clinical and molecular characterization of a novel mutation associated with severe thrombotic tendency.

  • https://pubmed.ncbi.nlm.nih.gov/28783511/
  • Anna Selmeczi, Réka Gindele, ..., Zsolt Oláh (2018). Thrombosis research. 2018 Aug 06
  • .. by FluoReporterlacZ/Galactosidase Quantitation Kit (Molecular Probes, Life Technologies) and the results were corrected accordingly.. Aliquots of the media and cell lysates were used for AT antigen determination by ELISA (AssayMax Human Antithrombin III ELISA Kit, Assaypro, St. Charles, MO) and for Western blotting.. AT activity from the conditioned and concentratedmedia of the transfected cells was determined by ..

Impact of enhanced metabolic stability on pharmacokinetics and pharmacodynamics of GalNAc–siRNA conjugates

  • https://pubmed.ncbi.nlm.nih.gov/28981809/
  • Jayaprakash K Nair, Husain Attarwala, ..., Martin A Maier (2017). Nucleic Acids Research. 2017 Nov 02
  • siAT-1 (10 mg/kg) or siAT-2 (1 and 10 mg/kg). At various time points, serum was collected and analysed for AT protein levels. AT protein levels were measured using the AssayMax Human ATIII ELISA Kit (AssayPro, St. Charles, MO, USA) as per manufacturer's instructions ( 25 ). Protein levels from individual animals at each time point were normalized to their respective individual pre-dose level (aver

Recurrent mutations in a SERPINC1 hotspot associate with venous thrombosis without apparent antithrombin deficiency

  • https://pubmed.ncbi.nlm.nih.gov/29542734/
  • Wei Zeng, Bei Hu, ..., Yu Hu (2017). Oncotarget. 2017 Oct 13
  • .. to generate the reference sample used to build the standard curve for the assays.. Immunochemical determination of antithrombin antigen levels was performed using a Human Antithrombin III ELISA Kit (AssayPro, St. Charles, MO, USA).. Functional and antigenic assays were done in plasma from 20 carriers of SERPINC1 mutations ..

Dual inhibition of thrombin and activated factor X attenuates disseminated intravascular coagulation and protects organ function in a baboon model of severe Gram-negative sepsis

  • https://pubmed.ncbi.nlm.nih.gov/28288667/
  • Herbert Schöchl, Martijn van Griensven, ..., Heinz Redl (2017). Critical Care. 2017 Mar 13
  • Coagulation tests The coagulation tests were performed according to the manufacturer’s instructions: antithrombin (AT) (AssayMax human Antithrombin III ELISA; AssayPro, St. Charles, MO, USA); D-dimer (Asserachrom D-Dimer ELISA, Diagnostica Stago, Taverny, France); fibrinogen (Human Fibrinogen ELISA, ICL, Portland, USA); PAI-1 antigen (Technozym ELISA, Cryopep, France); activated protein C (human a

An RNAi therapeutic targeting antithrombin to rebalance the coagulation system and promote hemostasis in hemophilia

  • https://pubmed.ncbi.nlm.nih.gov/25849132/
  • Alfica Sehgal, Scott Barros, ..., Akin Akinc (2015). Nature medicine. 2015 Apr 13
  • Abstract: Hemophilia A and B are inherited bleeding disorders characterized by deficiencies in procoagulant factor VIII (FVIII) or factor IX (FIX), respectively. There remains a substantial unmet medi…

Genetic analysis should be included in clinical practice when screening for antithrombin deficiency

  • https://pubmed.ncbi.nlm.nih.gov/25298121/
  • Yi-Qing Li, Tao Guo, ..., Yu Hu (2015). Thrombosis and haemostasis. 2015 Mar 01
  • In the study aimed at enhancing clinical practices for antithrombin deficiency screening, the Human Antithrombin III AT3 AssayMax ELISA Kit from Assaypro played a significant role by e…

High-molecular-weight kininogen and the intrinsic coagulation pathway in patients with de novo acute myocardial infarction

  • https://pubmed.ncbi.nlm.nih.gov/24096571/
  • Judit Cubedo, Ilaria Ramaiola, ..., Lina Badimon (2013). Thrombosis and haemostasis. 2013 Jan 01
  • Abstract: After an acute ischaemic event serum proteins may change reflecting the ischaemic damage. Proteomic studies could provide new insights into potential biomarkers in the evolution of ischaemic…

Plasma and urine proteomic profiles in childhood idiopathic nephrotic syndrome

  • https://pubmed.ncbi.nlm.nih.gov/22927352/
  • René Frydensbjerg Andersen, Johan Palmfeldt, ..., Søren Rittig (2012). Proteomics. Clinical applications. 2012 Aug 01
  • Abstract: Idiopathic nephrotic syndrome (NS) is characterized by proteinuria, edema, and hypoalbuminemia. The aim of the study was to identify differentially expressed proteins in urine and plasma dur…

Activity-dependent neuroprotector homeobox protein: A candidate protein identified in serum as diagnostic biomarker for Alzheimer's disease

  • https://pubmed.ncbi.nlm.nih.gov/22554909/
  • Ming-Hui Yang, Yuan-Han Yang, ..., Yu-Chang Tyan (2012). Journal of proteomics. 2012 Jun 01
  • Abstract: Alzheimer's disease (AD) is the most common cause of dementia of late life. To enhance our understanding of AD proteome, the serum proteins were analyzed using two-dimensional gel electropho…

Combined Proteomic and Metabolomic Profiling of Serum Reveals Association of the Complement System with Obesity and Identifies Novel Markers of Body Fat Mass Changes

  • https://pubmed.ncbi.nlm.nih.gov/21823675/
  • Andreas Oberbach, Matthias Blüher (2011). Journal of Proteome Research. 2011 Aug 29
  • Abstract: Obesity is associated with multiple adverse health effects and a high risk of developing metabolic and cardiovascular diseases. Therefore, there is a great need to identify circulating param…

Identification of Novel Diagnostic Biomarkers for Asthma and Chronic Obstructive Pulmonary Disease

  • https://pubmed.ncbi.nlm.nih.gov/21471098/
  • Nicole M Verrills, Jennifer A Irwin, ..., Peter G Gibson (2011). American journal of respiratory and critic…. 2011 Jun 15
  • Abstract: Proteomics may identify a useful panel of biomarkers for identification of asthma and chronic obstructive pulmonary disease (COPD). To conduct an unsupervised analysis of peripheral blood pr…

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