Human Elastase (Neutrophil ELA2) ELISA Kit

$407.00

Catalog Number: EE1001-1

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
Product Size:96 Well Plate

Additional information

Format

Sandwich ELISA

Entrez Gene

1991

Components

Biotinylated Human Elastase Antibody, Chromogen Substrate (1x), EIA Diluent Concentrate (10x), Human Elastase Microplate, Human Elastase Standard, Sealing Tapes, SP Conjugate (100x), Stop Solution (1x), Wash Buffer Concentrate (20x)

TIme

4 hours

Sensitivity

18 pg/ml

Range

0.078 – 5 ng/ml

Samples Type

CSF, Milk, Plasma, Saliva, Serum, Urine

Associated Disease

Microbiology, Neutropenia, Pulmonary Emphysema

Species

Human

UniProt

P08246

Storage

Refer to component labels for details

Plex

1

Usage

For Research Use Only, Not To Be Used For Diagnostic Purposes

Documentation

Publications citing Assaypro: Human Elastase (Neutrophil ELA2) ELISA Kit... (15 results)

Please use discretion when following external links; Assaypro cannot guarantee the validity and security of all citation links provided.

The porcine abattoir blood model: Bridging the gap between human and porcine blood for in-vitro testing

Mature neutrophils suppress T cell immunity in the tumor microenvironment of advanced epithelial ovarian cancer

Association of Aneurysm Tissue Neutrophil Mediator Levels with Intraluminal Thrombus Thickness in Patients with Abdominal Aortic Aneurysm

Relationship Between Arteriovenous Fistula Stenosis and Circulating Levels of Neutrophil Granule Proteins in Chronic Hemodialysis Patients

Differential effects of Th17 cytokines during the response of neutrophils to Burkholderia cenocepacia outer membrane protein A

Personalised anti-inflammatory therapy for bronchiectasis and cystic fibrosis: selecting patients for controlled trials of neutrophil elastase inhibition

The Burkholderia cenocepacia OmpA-like protein BCAL2958: identification,characterization, and detection of anti-BCAL2958 antibodies in serum from B. cepacia complex-infected Cystic Fibrosis patients

An ex vivo evaluation of blood coagulation and thromboresistance of two extracorporeal circuit coatings with reduced and full heparin dose

Association of Aneurysm Tissue Neutrophil Mediator Levels with Intraluminal Thrombus Thickness in Patients with Abdominal Aortic Aneurysm

  • https://pubmed.ncbi.nlm.nih.gov/35204755/
  • Aldona Siennicka, Monika Adamowicz, ..., Hong S. Lu (2022). Biomolecules. 2022 Feb 04
  • Blood and the aortic tissue homogenates were tested for MPO (Myeloperoxidase ELISA, DRG International, Springfield, NJ, USA), NGAL (Human NGAL ELISA Kit, BioPorto Diagnostics, Needham, MA, USA), neutrophil elastase (AssayMax Human Neutrophil elastase ELISA Kit, AssayPro St. Charles, MO, USA), IL-6 (IL-6 ELISA, DRG International, Inc. Springfield, NJ, USA), and TNF-a (AssayMax Human TNF-alpha ELISA Kit, AssayPro St. Charles, MO, USA) using commercially available enzyme-linked immunosorbent assays (ELISAs), according to the manufacturers’ instructions.

Relationship between arteriovenous fistula stenosis and circulating levels of neutrophil granule proteins in chronic hemodialysis patients

  • https://pubmed.ncbi.nlm.nih.gov/34437969/
  • Dong-Jin Oh, Jeong Hoon Lee, Hye Min Choi (2021). Annals of vascular surgery. 2021 Aug 01
  • Abstract: Background: Arteriovenous fistula (AVF) stenosis leading to its failure is a major cause of morbidity in hemodialysis patients; however, detailed pathogenesis of AVF stenosis is still under …

α 1 Antitrypsin therapy modulates the neutrophil membrane proteome and secretome.

  • https://pubmed.ncbi.nlm.nih.gov/32060059/
  • Mark P Murphy, Thomas McEnery, ..., Noel G McElvaney (2021). The European Respiratory Journal. 2021 Jun 21
  • .. immune band intensity of GAPDH in whole cell lysates of cells used per reaction.. Alternatively, secreted MPO and NE was analysed by ELISA using a Human MPO ELISA (R&D Systems; DY3174) and a Human Elastase (Neutrophil ELA2) AssayMax Elisa (Assaypro, EE1001-1)according to the manufacturer’s instructions and as previously reported [12].. Fluorescence resonance energy transfer (FRET) analysis FRET was performed according to a protocol by ..

Cardiopulmonary bypass and internal thoracic artery: Can roller or centrifugal pumps change vascular reactivity of the graft? The IPITA study: A randomized controlled clinical trial

  • https://pubmed.ncbi.nlm.nih.gov/32645079/
  • Olivier Fouquet, Simon Dang Van, Pauline Robert (2020). PLoS ONE. 2020 Jul 09
  • .. complement complex activation, and elastase and were collected at Time 1 and Time 2.. Enzyme-linked immunosorbent assay techniques were used from 10 μl of plasma to measure terminal complement (SC5b-9; Quidel, San Diego, CA, USA) and 50 μl of plasma for neutrophil elastase (Neutrophil ELA2, Assay pro, St Charles, USA).. The limit of sensitivity of each assay undertaken was as follows: SC5b-9 = 16 ..

Personalised anti-inflammatory therapy for bronchiectasis and cystic fibrosis: selecting patients for controlled trials of neutrophil elastase inhibition

  • https://pubmed.ncbi.nlm.nih.gov/30918898/
  • Holly R Keir, Christopher J Fong, ..., James D Chalmers (2019). ERJ Open Research. 2019 Mar 25
  • ergy transfer (FRET) assay according to the protocol of Korkmaz et al . [ 24 ], 2) an activity-based immunoassay (ProteaseTag ELISA; ProAxsis, Belfast, UK) [ 25 ] and 3) a total NE ELISA (AssayMax; Assaypro, St Charles, MO, USA), which measures total protein rather than activity. For the FRET assay, the intra-assay coefficient of variation was 5.6% and the interassay coefficient of variation was

The Burkholderia cenocepacia OmpA-like protein BCAL2958: identification, characterization, and detection of anti-BCAL2958 antibodies in serum from B. cepacia complex-infected Cystic Fibrosis patients

  • https://pubmed.ncbi.nlm.nih.gov/27325348/
  • Sílvia A Sousa, Mostafa Morad, ..., Jorge H Leitão (2016). AMB Express. 2016 Jun 21
  • metric kit (Bio-diagnostic, Egypt) according to Aebi (1984). Human myeloperoxidase (MPO), Tumor necrosis factor-α (TNF-α) and Neutrophil elastase were measured using the ELISA kits MPO (Boster Immunoleader, USA), Human TNF-α (Boster Immunoleader, USA) and elastase (ASSAY PRO, USA), respectively, according to the manufacturer’s instructions. Non-activated neutrophils were used as negative controls.

An ex vivo evaluation of blood coagulation and thromboresistance of two extracorporeal circuit coatings with reduced and full heparin dose.

  • https://pubmed.ncbi.nlm.nih.gov/24632424/
  • Leylah Teligui, Emilie Dalmayrac, ..., Christophe Baufreton (2015). Interactive cardiovascular and thoracic su…. 2015 Jan 12
  • .. 1 + 2 (F1.2) and Factor XIIa (Euromedex, Uscn Life Science, Inc., Souffelweyersheim, France).. Leucocyte activation was assessed through neutrophil elastase release and analysed using ELISA kits (AssayMax Human Neutrophil Elastase, AssayPro, St Charles, MO, USA)

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