Human Factor IX (Factor 9) ELISA Kit (with Control)

$453.00

Catalog Number: EF1009-7

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
Product Size:96 Well Plate

Additional information

Format

Sandwich ELISA

Entrez Gene

2158

Components

Biotinylated Human Factor IX Antibody, Chromogen Substrate (1x), EIA Diluent Concentrate (10x), Human Factor IX Microplate, Human Factor IX Standard, Positive Control, Sealing Tapes, SP Conjugate (100x), Stop Solution (1x), Wash Buffer Concentrate (20x)

TIme

4 hours

Sensitivity

0.5 ng/ml

Range

1.56 – 100 ng/ml; 0.313 – 20 mIU/ml

Samples Type

CSF, Plasma, Serum

Associated Disease

Blood Disorder

Species

Human

UniProt

P00740

Storage

Refer to component labels for details

Plex

1

Usage

For Research Use Only, Not To Be Used For Diagnostic Purposes

Documentation

Publications citing Assaypro: Human Factor IX (Factor 9) ELISA Kit ... (39 results)

Please use discretion when following external links; Assaypro cannot guarantee the validity and security of all citation links provided.

A comprehensive model for assessment of liver stage therapies targeting Plasmodium vivax and Plasmodium falciparum

  • https://www.nature.com/articles/s41467-018-04221-9
  • A Roth, SP Maher, AJ Conway, R Ubalee...- Nature..., 2018 - nature.com
  • Malaria liver stages represent an ideal therapeutic target with a bottleneck in parasite load and reduced clinical symptoms; however, current in vitro pre-erythrocytic (PE) models for...

Selective Microvascular Tissue Transfection Using Minicircle DNA for Systemic Delivery of Human Coagulation Factor IX in a Rat Model Using a Therapeutic Flap

Endoscopic-mediated, biliary hydrodynamic injection mediating clinically relevant levels of gene delivery in pig liver

Plasma Proteome Analyses of Experimental Human and Murine Thyroid Disease Models

Systemic delivery of factor IX messenger RNA for protein replacement therapy

Therapeutic efficacy in a hemophilia B model using a biosynthetic mRNA liver depot system

Lipid formulations for delivery of messenger rna

Development of an efficient human hepatocyte culture platform for assessing novel therapeutic efficacy against Plasmodium liver parasites

Mrna therapeutic compositions and use to treat diseases and disorders

Lipidic nanoparticles for mrna delivering

Early detection of malignant pleural mesothelioma in asbestos-exposed individuals with a noninvasive proteomics-based surveillance tool

Lipid nanoparticle compositions and methods for mrna delivery

Long-Acting Coagulation Factors and Methods of Producing Same

Sex hormones affect the production of recombinant Factor IX in CHO and HEK-293 cell lines

Transplacental delivery of factor IX Fc-fusion protein ameliorates bleeding phenotype of newborn hemophilia B mice.

  • https://pubmed.ncbi.nlm.nih.gov/39181162/
  • Fuminori Sakurai, Shunsuke Iizuka, ..., Hiroyuki Mizuguchi (2024). Journal of controlled release : official j…. 2024 Aug 24
  • .. were determined as previously described [14].Plasma FIX activities were determined as previously described [14].. In brief, plasma FIX levels were measured using an AssayMax Human Factor IX ELISA Kit (ASSAYPRO, St. Charles, MO) or were sensitively measured according to the method of Barzel et al. [20].. First, 100 μL of mouse antihuman FIX antibody (F2645; Sigma-Aldrich, St. Louis, MO) which ..

Patent Lipid nanoparticle compositions and methods for MRNA delivery

  • No Link Provided
  • Frank DeRosa, Braydon Charles Guild, Michael Heartlein (2024). us11951179. 2024 Apr 09
  • .. mouse serum and human Alpha-galactosidase protein were used as negative and positive controls, respectively.. FIXELISA: Quantification of FIX protein was performed following procedures reported for human FIX ELISA kit (AssayMax, Assay Pro, Catalog #EF1009-1).. A1AT ELISA: Quantification of A1AT protein was performed following procedures reported for human AlAT ..

Endoscopic-mediated, biliary hydrodynamic injection mediating clinically relevant levels of gene delivery in pig liver

  • https://pubmed.ncbi.nlm.nih.gov/34197834/
  • Robert L Kruse, Yuting Huang, ..., Vivek Kumbhari (2021). Gastrointestinal endoscopy. 2021 Jun 29
  • Abstract: Gene therapy could provide curative therapies to many inherited monogenic liver diseases. Clinical trials have largely focused on adeno-associated viruses (AAVs) for liver gene delivery. The…

Endoscopic-mediated, biliary hydrodynamic injection mediating clinically relevant levels of gene delivery in pig liver

  • https://pubmed.ncbi.nlm.nih.gov/34197834/
  • Robert L Kruse, Yuting Huang, ..., Vivek Kumbhari (2021). Gastrointestinal endoscopy. 2021 Jun 29
  • Abstract: Gene therapy could provide curative therapies to many inherited monogenic liver diseases. Clinical trials have largely focused on adeno-associated viruses (AAVs) for liver gene delivery. The…

Patent Lipid formulations for delivery of messenger RNA

  • No Link Provided
  • Michael Heartlein, Daniel Anderson, ..., Frank DeRosa (2021). us10959953. 2021 Mar 30
  • Enzyme-Linked Immunosorbent Assay (ELISA) Analysis A.Enzyme-Linked Immunosorbent Assay (ELISA) Analysis A.. Human FIX ELISA Quantification of FIX protein was performed following procedures reported for human FIX ELISA kit (AssayMax, Assay Pro, Catalog # EF1009-1).. Human ASS1 ELISA Standard ELISA procedures were followed employing mouse anti-ASS1 2D1-2E12 IgG as ..

Patent Long-acting coagulation factors and methods of producing same

  • No Link Provided
  • Udi Eyal Fima, Gili Hart (2021). us10960058. 2021 Mar 30
  • .. 20-fold and dialyzed with PBS using flow filtration cassette (10 KDa MWCO; Millipore Corp.).. Determination of FIX antigen level: FIX-CTP harvest antigen levels were determined using AssayMax Human FIX ELISA kit (AssayPro-EF1009-1).. The calculated protein concentration is the average of three different dilutions in two independent ..

CRISPR/Cas9-mediated gene correction in hemophilia B patient-derived iPSCs.

  • https://pubmed.ncbi.nlm.nih.gov/31664646/
  • Satoshi Morishige, Shinichi Mizuno, ..., Koji Nagafuji (2020). International journal of hematology. 2020 Aug 10
  • In this study, the Human Factor IX Factor 9 AssayMax ELISA Kit from Assaypro was pivotal for measuring FIX antigen levels in culture medium and cell lysates, confirming the successful …

A comprehensive model for assessment of liver stage therapies targeting Plasmodium vivax and Plasmodium falciparum

  • https://pubmed.ncbi.nlm.nih.gov/29884783/
  • Alison Roth, Steven P. Maher, ..., John H. Adams (2018). Nature Communications. 2018 May 09
  • .. Waltham, MA, USA) and the mean with s.d. ( n = 6) were reported.. Samples ( n = 3) were quantified for Factor IX ELISA per manufacturer’s protocols (Cat No. EF1009-1, AssayPro, St. Charles, MO, USA).. Cyotchrome P450 3a4 induction was measured using CYP3A4 P450 Glo™ luciferin-IPA kit (Cat No. ..

Neonatal Gene Therapy for Hemophilia B by a Novel Adenovirus Vector Showing Reduced Leaky Expression of Viral Genes

  • https://pubmed.ncbi.nlm.nih.gov/28828393/
  • Shunsuke Iizuka, Fuminori Sakurai, ..., Hiroyuki Mizuguchi (2017). Molecular Therapy. Methods & Clinical Deve…. 2017 Jul 08
  • .. followed by centrifugation (4°C, 7,000 × g , 5 min) to obtain the plasma.. The plasma hFIX antigen levels were measured using a Human Factor IX ELISA Kit (Assaypro). hFIX activity levels were measured using a KC4 Delta Coagulometer (Trinity Biotech).. In brief, 50 μL of the plasma samples were 10-fold diluted; mixed with 50 ..

Neonatal Gene Therapy for Hemophilia B by a Novel Adenovirus Vector Showing Reduced Leaky Expression of Viral Genes

  • https://pubmed.ncbi.nlm.nih.gov/28828393/
  • Shunsuke Iizuka, Fuminori Sakurai, ..., Hiroyuki Mizuguchi (2017). Molecular Therapy. Methods & Clinical Deve…. 2017 Jul 08
  • .. followed by centrifugation (4°C, 7,000 × g , 5 min) to obtain the plasma.. The plasma hFIX antigen levels were measured using a Human Factor IX ELISA Kit (Assaypro). hFIX activity levels were measured using a KC4 Delta Coagulometer (Trinity Biotech).. In brief, 50 μL of the plasma samples were 10-fold diluted; mixed with 50 ..

Neonatal Gene Therapy for Hemophilia B by a Novel Adenovirus Vector Showing Reduced Leaky Expression of Viral Genes

  • https://pubmed.ncbi.nlm.nih.gov/28828393/
  • Shunsuke Iizuka, Fuminori Sakurai, ..., Hiroyuki Mizuguchi (2017). Molecular Therapy. Methods & Clinical Deve…. 2017 Jul 08
  • .. followed by centrifugation (4°C, 7,000 × g , 5 min) to obtain the plasma.. The plasma hFIX antigen levels were measured using a Human Factor IX ELISA Kit (Assaypro). hFIX activity levels were measured using a KC4 Delta Coagulometer (Trinity Biotech).. In brief, 50 μL of the plasma samples were 10-fold diluted; mixed with 50 ..

First case report of hemophilia B Leyden in Japan.

  • https://pubmed.ncbi.nlm.nih.gov/28168417/
  • Atsuki Yamashita, Chiai Nagae, ..., Masashi Taki (2017). International journal of hematology. 2017 Jul 07
  • In the study of a Japanese patient with hemophilia B Leyden, the AssayMax Human Factor IX ELISA Kit from Assaypro was utilized to measure FIX antigen levels in historical samples. This…

Biodegradable hydrophilic carriers for the oral delivery of hematological factor IX for hemophilia B treatment.

  • https://pubmed.ncbi.nlm.nih.gov/27863665/
  • Sarena D Horava, Katie J Moy, Nicholas A Peppas (2017). International Journal of Pharmaceutics. 2017 Apr 14
  • In this study, the Human Factor IX Factor 9 AssayMax ELISA Kit from Assaypro played a critical role in measuring levels of factor IX, which was vital for assessing the effectiveness of…

Patent Pulmonary delivery of mRNA

  • No Link Provided
  • Braydon Charles Guild, Frank DeRosa, Michael Heartlein (2017). us09597413. 2017 Mar 21
  • .. mouse serum and human Alpha-galactosidase protein were used as negative and positive controls, respectively.. FIX ELISA: Quantification of FIX protein was performed following procedures reported for human FIX ELISA kit (AssayMax, Assay Pro, Catalog # EF1009-1).. A1AT ELISA: Quantification of A1AT protein was performed following procedures reported for human A1AT ..

Systemic delivery of factor IX messenger RNA for protein replacement therapy

  • https://pubmed.ncbi.nlm.nih.gov/28202722/
  • Suvasini Ramaswamy, Nina Tonnu, ..., Inder M Verma (2017). Proceedings of the National Academy of Sci…. 2017 Feb 15
  • Abstract: Safe and efficient delivery of messenger RNAs for protein replacement therapies offers great promise but remains challenging. In this report, we demonstrate systemic, in vivo, nonviral mRNA …

Systemic delivery of factor IX messenger RNA for protein replacement therapy

  • https://pubmed.ncbi.nlm.nih.gov/28202722/
  • Suvasini Ramaswamy, Nina Tonnu (2017). Proceedings of the National Academy of Sci…. 2017 Feb 15
  • .. once to 7-wk-old female balb/c mice at 2 mg/kg (n = 3 per group).. The animals were bled at 6-h postdosing and the serum FIX levels were assessed by an ELISA (Assay Pro: EF1009-1) at 1:200 dilution as per the manufacturer’s instructions.. As can be seen, the LUNAR formulation was up to two times more efficient ..

Therapeutic efficacy in a hemophilia B model using a biosynthetic mRNA liver depot system

  • https://pubmed.ncbi.nlm.nih.gov/27356951/
  • F DeRosa, B Guild, ..., M W Heartlein (2016). Gene therapy. 2016 Oct 01
  • .. via absorption (450 nm) on a Molecular Devices Flex Station instrument (Sunnyvale, CA, USA).. Quantification of FIX protein was performed following procedures reported for hFIX ELISA kit (AssayMax, Assay Pro, Catalog # EF1009-1; St Charles, MO, USA).. High specificity for the human forms of each respective protein was obtained with no ..

Therapeutic efficacy in a hemophilia B model using a biosynthetic mRNA liver depot system

  • https://pubmed.ncbi.nlm.nih.gov/27356951/
  • F DeRosa, B Guild, ..., M W Heartlein (2016). Gene therapy. 2016 Jun 30
  • ed via absorption (450 nm) on a Molecular Devices Flex Station instrument (Sunnyvale, CA, USA). Quantification of FIX protein was performed following procedures reported for hFIX ELISA kit (AssayMax, Assay Pro, Catalog # EF1009-1; St Charles, MO, USA). High specificity for the human forms of each respective protein was obtained with no cross-reactivity observed in mouse samples (EPO/FIX) and minima

Patent MRNA therapy for Fabry disease

  • No Link Provided
  • Braydon Charles Guild, Frank DeRosa, Michael Heartlein (2016). us09308281. 2016 Apr 12
  • Quantification of FIX protein was performed following procedures reported for human FIX ELISA kit (AssayMax, Assay Pro, Catalog # EF1009-1).

Design of pH-Responsive Biomaterials to Enable the Oral Route of Hematological Factor IX

  • https://pubmed.ncbi.nlm.nih.gov/26883955/
  • Sarena D. Horava, Nicholas A. Peppas (2016). Annals of biomedical engineering. 2016 Feb 16
  • .. VT).Plasma-derived human factor IX (hFIX) was purchased from Haematologic Technologies Inc. (Essex Junction, VT).. Human factor IX ELISA kits were purchased from AssayPro LLC. (St. Charles, MO), and the BIOPHEN factor IX activity assays were purchased from HYPHEN Biomed SAS (Neuville-sur-Oise, France).. FaSSIF, FeSSIF & FaSSGF Powder was purchased from Biorelevant.com (London, England, UK).FaSSIF, FeSSIF & ..

Early Detection of Malignant Pleural Mesothelioma in Asbestos-Exposed Individuals with a Noninvasive Proteomics-Based Surveillance Tool

  • https://pubmed.ncbi.nlm.nih.gov/23056237/
  • Rachel M. Ostroff, Michael R. Mehan, ..., Thomas Behrens (2012). PLoS ONE. 2012 Oct 03
  • Complement Factor 9 (MicroVue SC5b-9 EIA kit, Quidel Corporation, San Diego, CA USA), Factor IX (AssayMax Human Factor IX ELISA kit, AssayPro St. Charles, MO USA) and Human CXCL13 (Human CXCL13/BLC/BCA-1 Quantikine ELISA kit, R&D Systems, Minneapolis, MN USA) were analyzed in order to validate SOMAmer results in the 68 controls and 32 MMs in the blinded validation trial.

Early Detection of Malignant Pleural Mesothelioma in Asbestos-Exposed Individuals with a Noninvasive Proteomics-Based Surveillance Tool

  • https://pubmed.ncbi.nlm.nih.gov/23056237/
  • Rachel M Ostroff, Michael R Mehan, ..., Harvey I Pass (2012). PLoS ONE. 2012 Oct 03
  • Human L-ficolin ELISA kit (Hycult biotech, Uden, the Netherlands). Complement Factor 9 (MicroVue SC5b-9 EIA kit, Quidel Corporation, San Diego, CA USA), Factor IX (AssayMax Human Factor IX ELISA kit, AssayPro St. Charles, MO USA) and Human CXCL13 (Human CXCL13/BLC/BCA-1 Quantikine ELISA kit, R & D Systems, Minneapolis, MN USA) were analyzed in order to validate SOMAmer results in the 68 controls an

Sex hormones affect the production of recombinant Factor IX in CHO and HEK-293 cell lines

  • https://pubmed.ncbi.nlm.nih.gov/18581062/
  • Nazanin Dadehbeigi, Seyed Nasser Ostad, ..., Mohammad Hossein Ghahremani (2008). Biotechnology Letters. 2008 Jun 26
  • Abstract: Recombinant human Factor IX (rFIX) was cloned in a mammalian expression vector and transfected into CHO and HEK-293. Treatment with 10(-9) M methyl testosterone increased rFIX production by …

Sex hormones affect the production of recombinant Factor IX in CHO and HEK-293 cell lines

  • https://pubmed.ncbi.nlm.nih.gov/18581062/
  • Nazanin Dadehbeigi, Seyed Nasser Ostad, ..., Mohammad Hossein Ghahremani (2008). Biotechnology Letters. 2008 Jun 26
  • Abstract: Recombinant human Factor IX (rFIX) was cloned in a mammalian expression vector and transfected into CHO and HEK-293. Treatment with 10(-9) M methyl testosterone increased rFIX production by …

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