Human Lactoferrin ELISA Kit

$407.00

Catalog Number: EL1011-1

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
Product Size:96 Well Plate

Catalog Number. EL1011-1 Categories: , , Tag: Brand:

Additional information

Format

Competitive ELISA

Entrez Gene

4057

Components

Biotinylated Human Lactoferrin Protein, Chromogen Substrate (1x), EIA Diluent Concentrate (10x), Human Lactoferrin Microplate, Human Lactoferrin Standard, Sealing Tapes, SP Conjugate (100x), Stop Solution (1x), Wash Buffer Concentrate (20x)

TIme

2 hours

Sensitivity

0.15 µg/ml

Range

0.188 – 6 ug/ml

Samples Type

CSF, Milk, Plasma, Saliva, Serum

Associated Disease

Cancer, Crohn's Disease., Infectious Diarrhea, Inflammatory Bowel Disease, Pouchitis

Species

Human

UniProt

P02788

Storage

Refer to component labels for details

Plex

1

Usage

For Research Use Only, Not To Be Used For Diagnostic Purposes

Documentation

Publications

Please use discretion when following external links; Assaypro cannot guarantee the validity and security of all citation links provided.

The influence of lactoferrin in plasma and peritoneal fluid on iron metabolism in women with endometriosis

  • https://www.mdpi.com/1422-0067/24/2/1619
  • E Skarżyńska, M Wróbel, H Zborowska… - International journal of …, 2023 - mdpi.com
  • The aim of this study was to investigate the relationship between lactoferrin and iron and its binding proteins in women with endometriosis by simultaneously measuring these …

Variable concentration of ferritin in meconium in relation to the iron regulatory proteins and newborn body size

Variations in serum concentrations of C-reactive protein, ceruloplasmin, lactoferrin and myeloperoxidase and their interactions during normal human pregnancy and …

Systemic and mucosal levels of lactoferrin in very low birth weight infants supplemented with bovine lactoferrin

  • https://cdnsciencepub.com/doi/abs/10.1139/bcb-2020-0238
  • HL Itell, A Berenz, RJ Mangan… - … and Cell Biology, 2021 - cdnsciencepub.com
  • Lactoferrin supplementation may help prevent infections in preterm infants, but the efficacy has varied with different doses and products. We assessed the absorption and excretion of …

Relationship between salivary immunoglobulin a, lactoferrin and lysozyme flow rates and lifestyle factors in Japanese children: a cross-sectional study

  • https://www.tandfonline.com/doi/abs/10.1080/00016357.2016.1223340
  • M Ide, J Saruta, M To, Y Yamamoto… - Acta Odontologica …, 2016 - Taylor & Francis
  • … Briefly, sample and human lactoferrin standards (Assaypro LLC) were added to each well of a 96-well microplate and incubated at room temperature for 2 h. The excess primary …

Albumin fusion at the N-terminus or C-terminus of human lactoferrin leads to improved pharmacokinetics and anti-proliferative effects on cancer cell lines

  • https://www.sciencedirect.com/science/article/pii/S0928098720303390
  • K Ueda, M Shimizu, A Ohashi, D Murata… - European Journal of …, 2020 - Elsevier
  • Human lactoferrin (hLF), a soluble factor of the innate immune system, exhibits various biological functions and therefore has potential as a therapeutic protein. However, the clinical …

Hinge-deficient IgG1 Fc fusion: Application to human lactoferrin

  • https://pubs.acs.org/doi/abs/10.1021/acs.molpharmaceut.7b00221
  • Y Shiga, D Murata, A Sugimoto, Y Oshima… - Molecular …, 2017 - ACS Publications
  • Fusion of therapeutic proteins with the antibody Fc domain is a strategy widely applied to increase protein half-life in plasma. In our previous study, we generated a recombinant human …

Separation of lactoferrin from human saliva using monolithic disc

  • https://link.springer.com/article/10.1007/s10337-013-2459-x
  • O Zitka, S Skalickova, S Krizkova, M Vlkova, V Adam… - Chromatographia, 2013 - Springer
  • In this study, we optimized method for the isolation and detection of lactoferrin from human saliva using 3 mm short monolithic disc. We optimized the conditions for separation as flow …

Lactoferrin–endothelin-1 axis contributes to the development and invasiveness of triple-negative breast cancer phenotypes

Meconium proteins involved in iron metabolism

  • https://www.mdpi.com/1422-0067/25/13/6948
  • E Skarżyńska, A Jakimiuk, T Issat… - International Journal of …, 2024 - mdpi.com
  • The lack of specific biological materials and biomarkers limits our knowledge of the mechanisms underlying intrauterine regulation of iron supply to the fetus. Determining the meconium …

Correlations between ceruloplasmin, lactoferrin and myeloperoxidase in meconium

Correlation between the concentrations of lactoferrin and neutrophil gelatinase-associated lipocalin in meconium

  • https://link.springer.com/article/10.1007/s10534-017-0073-3
  • B Lisowska-Myjak, E Skarżyńska, P Wilczyńska… - Biometals, 2018 - Springer
  • Neutrophil gelatinase-associated lipocalin (NGAL) and lactoferrin (Lf) are among the key components of the innate immune system due to their ability to bind iron with high affinity and …

Recombinant human lactoferrin-Fc fusion with an improved plasma half-life

  • https://www.sciencedirect.com/science/article/pii/S0928098714004333
  • Y Shiga, Y Oshima, Y Kojima, A Sugimoto… - European Journal of …, 2015 - Elsevier
  • Lactoferrin (LF), an 80-kDa iron-binding glycoprotein found in mammalian exocrine secretions, has potential therapeutic efficacy due to its extensive health-promoting effects. However, …

The dominant expression of functional human lactoferrin in transgenic cloned goats using a hybrid lactoferrin expression construct

Relationships between meconium concentrations of acute phase proteins

  • https://onlinelibrary.wiley.com/doi/abs/10.1111/1440-1681.12995
  • B Lisowska‐Myjak, E Skarżyńska… - Clinical and …, 2018 - Wiley Online Library
  • Meconium concentrations of naturally accumulated ceruloplasmin (CER), lactoferrin (LF), and neutrophil gelatinase‐associated lipocalin (NGAL) and their relationships considered as a …

Lactoferrin levels in human breast milk among lactating mothers with sick and healthy babies in Kaduna State, Nigeria

Meconium lactoferrin levels in neonates: can we predefine normal values?

  • https://www.tandfonline.com/doi/abs/10.3109/14767058.2016.1171307
  • E Skarżyńska, J Żytyńska-Daniluk… - The Journal of …, 2017 - Taylor & Francis
  • Background and aims: In recent years, Lactoferrin (LF) has become an object of interest to neonatologists. To date, there have been no studies on the presence of LF in neonatal …

Establishing human lacrimal gland cultures with secretory function

Development of transgenic goat embryos expressing human lactoferrin gene

Concentrations of neutrophil-derived proteins in meconium and their correlations

  • https://www.tandfonline.com/doi/abs/10.2217/bmm-2016-0034
  • B Lisowska-Myjak, J Żytyńska-Daniluk… - Biomarkers in …, 2016 - Taylor & Francis
  • Aim The aim was to measure concentrations of four neutrophil-derived proteins in meconium as biomarkers describing prenatal environment. Methods Calprotectin, lactoferrin, …

Links between vitamin D-binding protein, alpha-1 antitrypsin and neutrophil proteins in meconium

Can aminopeptidase N determined in the meconium be a candidate for biomarker of fetal intrauterine environment?

  • https://www.sciencedirect.com/science/article/pii/S0014480020300125
  • B Lisowska-Myjak, P Wilczyńska, Z Bartoszewicz… - Experimental and …, 2020 - Elsevier
  • Aim To investigate possible association of aminopeptidase N/CD13 with other parameters of possible homeostatic mechanisms in meconium for potential use in identifying intrauterine …

Relationships between meconium concentrations of acute phase proteins

  • https://onlinelibrary.wiley.com/doi/abs/10.1111/1440-1681.12995
  • B Lisowska‐Myjak, E Skarżyńska… - Clinical and …, 2018 - Wiley Online Library
  • Meconium concentrations of naturally accumulated ceruloplasmin (CER), lactoferrin (LF), and neutrophil gelatinase‐associated lipocalin (NGAL) and their relationships considered as a …

Effects of heat-not-burn cigarette smoking on the secretion of saliva and its innate immune system components

  • https://www.mdpi.com/2227-9032/11/1/132
  • Y Mori, M Tanaka, H Kozai, Y Aoyama, Y Shigeno… - Healthcare, 2022 - mdpi.com
  • Saliva and salivary antimicrobial proteins play important roles in the innate immunity, which prevents infections of orally invading bacteria and viruses. In this study, we compared the …

Correlation Between Salivary Anxiety Markers and Salivary Biochemical Markers in Children with Primary and Mixed Dentition

  • https://dergipark.org.tr/en/pub/experimed/issue/50966/664869
  • S Oktay, S Demirel, ÜV Üstündağ, B Korkmaz… - Experimed, 2019 - dergipark.org.tr
  • DOI: 10.26650/experimed.2019.19021Objective: Anxiety due to the dentist and dental treatment is a problem encountered in many children. The aim of the present study is to determine …

Impact of lipid degradation and tear film stability on silicone hydrogel contact lens wearers with discomfort

  • https://www.sciencedirect.com/science/article/pii/S1367048426000123
  • T Itokawa, T Suzuki, K Kakisu, Y Hori - Contact Lens and Anterior Eye, 2025 - Elsevier
  • Purpose To investigate the potential impact of lipid degradation deposition and tear film stability on contact lens discomfort in silicone hydrogel soft contact lens (SCL) wearers. Methods …

Development of organotypic human breast cell cultures for high throughput testing

  • https://scholarworks.calstate.edu/downloads/0c483k034
  • A Agarwal - 2014 - scholarworks.calstate.edu
  • Breast cancer is not a disease of individual cells, but a collective failure of cells and tissues to communicate properly. Malignant transformation of breast cells is a complex multi-stage …

ANALYTICAL PERFORMANCE OF NOVEL ASSAY FOR MEASURING SEX HORMONE-BINDING GLOBULIN (SHBG) USING FLUORESCENCE IMMUNOASSAY BY …

小児における唾液中抗菌物質の動態と生活習慣との関連

  • https://kdu.repo.nii.ac.jp/record/955/files/kou489_summary.pdf
  • 井出桃, イデモモ - 2016 - kdu.repo.nii.ac.jp
  • 成人で 1 日に約 1.0~ 1.5 ℓ 分泌される唾液は, 口腔内を湿潤状態に保ち, 咀嚼, 会話などの口腔 機能に重要な役割を果たしている. また唾液には洗浄作用, 再石灰化作用, 緩衝作用, 消化作用, 抗菌…

* Mori Y et al. (2022) Effects of Heat-Not-Burn Cigarette Smoking on the Secretion of Saliva and Its Innate Immune System Components. Healthcare (Basel). 11(1):132.

* Cieslicka M et al. (2022) Effects of Long-Term Supplementation of Bovine Colostrum on Iron Homeostasis, Oxidative Stress, and Inflammation in Female Athletes: A Placebo-Controlled Clinical Trial. Nutrients. 15(1):186.

* Zhou X et al. (2021) Optimized lactoferrin as a highly promising treatment for intracerebral hemorrhage: pre-clinical experience. J Cereb Blood Flow Metab. 41(1): 53-66.

* Wu SI et al. (2021) Psychobiotic Supplementation of PS128TM Improves Stress, Anxiety, and Insomnia in Highly Stressed Information Technology Specialists: A Pilot Study. Front Nutr. 8:614105.

* Weimer K et al. (2021) Breast milk and saliva lactoferrin levels and postnatal cytomegalovirus infection. Am J Perinatol. 38(10):1070-1077

* Perea L et al. (2021) A cluster analysis of bronchiectasis patients based on the airway immune profile. Chest. 159(5):1758-1767.

* Itell HL et al. (2021) Systemic and mucosal levels of lactoferrin in very low birth weight infants supplemented with bovine lactoferrin. Biochem Cell Biol. 99(1):25-34.

* Ahmad HS et al. (2021) Effects of an Acute Bout of Exercise on Salivary Lactoferrin Responses among Smokers and Non-Smokers. J. Sci. Tech. 13(1):12-17.

* Ochoa TJ et al. (2020) Randomized Controlled Trial of Bovine Lactoferrin for Prevention of Sepsis and Neurodevelopment Impairment in Infants <2000 G. J Pediatr. 219: 118-125.

* Ochoa TJ (2020) Is Mothers Own Milk Lactoferrin Intake Associated with Reduced Neonatal Sepsis, Necrotizing Enterocolitis, and Death? Neonatology. 117(2):167-174.

* Morsi MS et al. (2020) Effect of Aerobic and Arm Exercises on HbA1c and Immunological Properties of Breast Milk in Diabetic Lactating Women. J. Environ. Treat. Tech. 8(4):1420-1424.

* Lisowska-Myjak B et al. (2020) Can aminopeptidase N determined in the meconium be a candidate for biomarker of fetal intrauterine environment? Exp Mol Pathol. 115:104446.

* Huot S et al. (2020) IgG-aggregates rapidly up-regulate FcgRI expression at the surface of human neutrophils in a FcgRII-dependent fashion: A crucial role for FcgRI in the generation of reactive oxygen species. FASEB J. 34(11):15208-15221.

* Huot S (2020) Understanding the aberrant responses of neutrophils in lupus for the development of new therapeutic approaches. Laval University.

* Sibila O et al. (2019) Antimicrobial peptides, disease severity and exacerbations in bronchiectaiss. Thorax. 74(9):835-842.

* Nieto JC et al. (2018) Ascitic fluid regulates the local innate immune responseof patients with cirrhosis. J Leukoc Biol. 104(4):833-841.

* Madani H et al. (2018) Ascitic Fluid Markers Hepcidin, Calprotectin, and Lactoferrin in Early Diagnosis and Follow-up of Spontaneous Bacterial Peritonitis. Med. J. Cairo Univ. 86(7): 3543-3549.

* Lisowska-Myjak B et al. (2018) Correlation between the concentrations of lactoferrin and neutrophil gelatinase-associated lipocalin in meconium. Biometals (2018) 31:123-129.

* Georgantas III RW et al. (2018) PREDICTION OF CLINICAL RESPONSE TO IL23-ANTAGONISTS USING IL23 PATHWAY BIOMARKERS. United States Patent Application 20180252728

* Turin CG et al. (2017) Lactoferrin concentration in breast milk of mothers of low-birth-weight newborns. J Perinatol. 37(5): 507-512.

* Skarzynska E et al. (2017) Meconium lactoferrin levels in neonates: can we predefine normal values? J Matern Fetal Neonatal Med. 30(3):279-283.

* Skarzynska E et al. (2017) Correlations between ceruloplasmin, lactoferrin and myeloperoxidase in meconium. J Trace Elem Med Biol. 43:58-62.

* Perrin MT et al. (2017) A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking. Matern Child Nutr. 13(1):e12239.

* Mira A et al. (2017) Salivary Immune and Metabolic Marker Analysis (SIMMA): A Diagnostic Test to Predict Caries Risk. Diagnostics (Basel). 7(3):38.

* Marcil V et al. (2017) Cardiometabolic risk factors and lactoferrin: Polymorphisms and plasma levels in French-Canadian children. Pediatr Res. 82(5):741-748.

* Denk S et al. (2017) Complement C5a Functions as a Master Switch for the pH Balance in Neutrophils Exerting Fundamental Immunometabolic Effects. J Immunol. 198(12):4846-4854.

* Daniel J et al. (2017) Products derived from amniotic fluid and methods of use. Patent Application: WO 2017003954 A1

* Mehta M et al. (2016) A Prospective Study of Iron Status of Exclusively Breastfed Infants Weighing 1800-2499g At Birth and Correlation With Breast Milk Lactoferrin. International Journal of Pediatrics and Child Health 4: 42-51.

* Mayeur S et al. (2016) Plasma Lactoferrin Levels Positively Correlate with Insulin Resistance despite an Inverse Association with Total Adiposity in Lean and Severely Obese Patients. PLoS One. 11(11):e0166138

* Lisowska-Myjak B et al. (2016) Concentrations of neutrophil-derived proteins in meconium and their correlations.Biomark Med. 10(8):819-29.

* Ide M et al. (2016) Relationship between salivary immunoglobulin a, lactoferrin and lysozyme flow rates and lifestyle factors in Japanese children: a cross-sectional study. Acta Odontol Scand. 74(7):576-583.

* Denk S (2016) Complement C5a-induced Functional change in neutrophil granulocytes during systemic inflammation. University of Ulm.

* Yuksel S et al. (2015) Oxidant and antioxidant status of human breast milk during lactation period. Dairy Sci. Technol. (2015) 95:295-302.

* Woods CM et al. (2015) The effect of nasal irrigation formulation on the antimicrobial activity of nasal secretions. Int Forum Allergy Rhinol. 5(12):1104-1110

* Skarzynska E (2015) LACTOFERRIN ACCUMULATION IN FETAL INTESTINE. Clin. Chem. Lab Med 53 Special Suppl:S798

* Shiga Y et al. (2015) Recombinant human lactoferrin-Fc fusion with an improved plasma half-life. Eur J Pharm Sci. 67:136-143

* Sato A et al. (2015) Lactoferrin fusion protein and method for preparation thereof. Patent application: US 14/395,826, US20150093382 A1

* Glennie S et al. (2015) Modulation of nasopharyngeal innate defenses by viral coinfection predisposes individuals to experimental pneumococcal carriage. Mucosal Immunol. 2015 Apr 29

* Gillum T et al. (2015) Exercise, But Not Acute Sleep Loss, Increases Salivary Antimicrobial Protein Secretion. J Strength Cond Res. 29(5):1359-66.

* Nienhouse V et al. (2014) Interplay between Bladder Microbiota and Urinary Antimicrobial Peptides: Mechanisms for Human Urinary Tract Infection Risk and Symptom Severity.PLoS One. 9(12):e114185.

* Moastafa TM et al. (2014) International Scholarly Research Notices. Study on the Therapeutic Benefit on Lactoferrin in Patients with Colorectal Cancer Receiving Chemotherapy. Volume 2014 (2014), Article ID 184278, 10 pages

* Gillum T et al. (2014) The effects of exercise, sex, and menstrual phase on salivary antimicrobial proteins. Exerc Immunol Rev. 20:23-38

* Zitka O et al. (2013) Separation of Lactoferrin from Human Saliva Using Monolithic Disc. Chromatographia 76:611-619

* Gillum TL et al. (2013) Salivary antimicrobial protein response to prolonged running. Biol Sport. 30(1):3-8.

* Yu H et al. (2012) The dominant expression of functional human lactoferrin in transgenic cloned goats using a hybrid lactoferrin expression construct. J Biotechnol. 161(3):198-205

* Tiwari S et al. (2012) Establishing Human Lacrimal Gland Cultures with Secretory Function. PLoS One. 7(1): e29458

* Selimoglu MA et al. (2012) The role of fecal calprotectin and lactoferrin in the diagnosis of necrotizing enterocolitis. Pediatr Crit Care Med. 13(4):452-454

* Ha NH et al. (2011) Lactoferrin-Endothelin-1 Axis Contributes to the Development and Invasiveness of Triple-Negative Breast Cancer Phenotypes. Cancer Res. 71(23):7259-7269

* Ella EE et al. (2011) Studies on the interaction between IgA, lactoferrin and lysozyme in the breastmilk of lactating women with sick and healthy babies. Lactoferrin and lysozyme. Journal of Infectious Diseases and Immunity 3(2): 24-29

* Eming SA et al. (2010) Differential Proteomic Analysis Distinguishes Tissue Repair Biomarker Signatures in Wound Exudates Obtained from Normal Healing and Chronic Wounds. J Proteome Res. 9(9):4758-4766

* Beaumont M et al. (2010) INFLAMMATORY BOWEL DISEASE BIOMARKERS AND RELATED METHODS OF TREATMENT. Patent application: PCT/US2009/062541. Publication number: WO2010062663 A1

* Soler-Garcia AA et al. (2009) Iron-Related Proteins: Candidate Urine Biomarkers in Childhood HIV-Associated Renal Diseases. Clin J Am Soc Nephrol 4:763-771

* Ella EE et al. (2009) Lactoferrin levels in human breast milk among lactating mothers with sick and healthy babies in Kaduna State, Nigeria. Int. J. Med. Medical Sciences 1(11):495-500

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