Human Lactoferrin ELISA Kit

$407.00

Catalog Number: EL1011-1

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
Product Size:96 Well Plate

Catalog Number. EL1011-1 Categories: , , Tag: Brand:

Additional information

Format

Competitive ELISA

Entrez Gene

4057

Components

Biotinylated Human Lactoferrin Protein, Chromogen Substrate (1x), EIA Diluent Concentrate (10x), Human Lactoferrin Microplate, Human Lactoferrin Standard, Sealing Tapes, SP Conjugate (100x), Stop Solution (1x), Wash Buffer Concentrate (20x)

TIme

2 hours

Sensitivity

0.15 µg/ml

Range

0.188 – 6 ug/ml

Samples Type

CSF, Milk, Plasma, Saliva, Serum

Associated Disease

Cancer, Crohn's Disease., Infectious Diarrhea, Inflammatory Bowel Disease, Pouchitis

Species

Human

UniProt

P02788

Storage

Refer to component labels for details

Plex

1

Usage

For Research Use Only, Not To Be Used For Diagnostic Purposes

Documentation

Publications citing Assaypro: Human Lactoferrin ELISA Kit... (110 results)

Please use discretion when following external links; Assaypro cannot guarantee the validity and security of all citation links provided.

The influence of lactoferrin in plasma and peritoneal fluid on iron metabolism in women with endometriosis

  • https://www.mdpi.com/1422-0067/24/2/1619
  • Ewa Skarżyńska, Monika Wróbel, ..., Piotr Laudański (2023). International Journal of Molecular Science…. 2023 Jan 24
  • The aim of this study was to investigate the relationship between lactoferrin and iron and its binding proteins in women with endometriosis by simultaneously measuring these

Variable concentration of ferritin in meconium in relation to the iron regulatory proteins and newborn body size

Variations in serum concentrations of C-reactive protein, ceruloplasmin, lactoferrin and myeloperoxidase and their interactions during normal human pregnancy and

Systemic and mucosal levels of lactoferrin in very low birth weight infants supplemented with bovine lactoferrin

  • https://cdnsciencepub.com/doi/abs/10.1139/bcb-2020-0238
  • HL Itell, A Berenz, RJ Mangan - and Cell Biology, 2021 - cdnsciencepub.com
  • Lactoferrin supplementation may help prevent infections in preterm infants, but the efficacy has varied with different doses and products. We assessed the absorption and excretion of

Relationship between salivary immunoglobulin a, lactoferrin and lysozyme flow rates and lifestyle factors in Japanese children: a cross-sectional study

Albumin fusion at the N-terminus or C-terminus of human lactoferrin leads to improved pharmacokinetics and anti-proliferative effects on cancer cell lines

Hinge-deficient IgG1 Fc fusion: Application to human lactoferrin

  • https://pubs.acs.org/doi/abs/10.1021/acs.molpharmaceut.7b00221
  • Y Shiga, D Murata, A Sugimoto, Y Oshima - Molecular , 2017 - ACS Publications
  • Fusion of therapeutic proteins with the antibody Fc domain is a strategy widely applied to increase protein half-life in plasma. In our previous study, we generated a recombinant human

Separation of lactoferrin from human saliva using monolithic disc

  • https://link.springer.com/article/10.1007/s10337-013-2459-x
  • O Zitka, S Skalickova, S Krizkova, M Vlkova, V Adam - Chromatographia, 2013 - Springer
  • In this study, we optimized method for the isolation and detection of lactoferrin from human saliva using 3 mm short monolithic disc. We optimized the conditions for separation as flow

Lactoferrinendothelin-1 axis contributes to the development and invasiveness of triple-negative breast cancer phenotypes

Meconium proteins involved in iron metabolism

  • https://www.mdpi.com/1422-0067/25/13/6948
  • E Skarżyńska, A Jakimiuk, T Issat - International Journal of , 2024 - mdpi.com
  • The lack of specific biological materials and biomarkers limits our knowledge of the mechanisms underlying intrauterine regulation of iron supply to the fetus. Determining the meconium

Correlations between ceruloplasmin, lactoferrin and myeloperoxidase in meconium

Correlation between the concentrations of lactoferrin and neutrophil gelatinase-associated lipocalin in meconium

  • https://link.springer.com/article/10.1007/s10534-017-0073-3
  • B Lisowska-Myjak, E Skar?y?ska, P Wilczy?ska - Biometals, 2018 - Springer
  • Neutrophil gelatinase-associated lipocalin (NGAL) and lactoferrin (Lf) are among the key components of the innate immune system due to their ability to bind iron with high affinity and

Recombinant human lactoferrin-Fc fusion with an improved plasma half-life

The dominant expression of functional human lactoferrin in transgenic cloned goats using a hybrid lactoferrin expression construct

Relationships between meconium concentrations of acute phase proteins

  • https://onlinelibrary.wiley.com/doi/abs/10.1111/1440-1681.12995
  • B Lisowska-Myjak, E Skar?y?ska - Clinical and , 2018 - Wiley Online Library
  • Meconium concentrations of naturally accumulated ceruloplasmin (CER), lactoferrin (LF), and neutrophil gelatinase-associated lipocalin (NGAL) and their relationships considered as a

Lactoferrin levels in human breast milk among lactating mothers with sick and healthy babies in Kaduna State, Nigeria

Meconium lactoferrin levels in neonates: can we predefine normal values?

Establishing human lacrimal gland cultures with secretory function

Development of transgenic goat embryos expressing human lactoferrin gene

Concentrations of neutrophil-derived proteins in meconium and their correlations

  • https://www.tandfonline.com/doi/abs/10.2217/bmm-2016-0034
  • B Lisowska-Myjak, J ?yty?ska-Daniluk - Biomarkers in , 2016 - Taylor & Francis
  • Aim The aim was to measure concentrations of four neutrophil-derived proteins in meconium as biomarkers describing prenatal environment. Methods Calprotectin, lactoferrin,

Links between vitamin D-binding protein, alpha-1 antitrypsin and neutrophil proteins in meconium

Can aminopeptidase N determined in the meconium be a candidate for biomarker of fetal intrauterine environment?

Effects of heat-not-burn cigarette smoking on the secretion of saliva and its innate immune system components

  • https://www.mdpi.com/2227-9032/11/1/132
  • Y Mori, M Tanaka, H Kozai, Y Aoyama, Y Shigeno - Healthcare, 2022 - mdpi.com
  • Saliva and salivary antimicrobial proteins play important roles in the innate immunity, which prevents infections of orally invading bacteria and viruses. In this study, we compared the

Correlation Between Salivary Anxiety Markers and Salivary Biochemical Markers in Children with Primary and Mixed Dentition

  • https://dergipark.org.tr/en/pub/experimed/issue/50966/664869
  • S Oktay, S Demirel, V stnda?, B Korkmaz - Experimed, 2019 - dergipark.org.tr
  • DOI: 10.26650/experimed.2019.19021Objective: Anxiety due to the dentist and dental treatment is a problem encountered in many children. The aim of the present study is to determine

Impact of lipid degradation and tear film stability on silicone hydrogel contact lens wearers with discomfort

  • https://www.sciencedirect.com/science/article/pii/S1367048426000123
  • T Itokawa, T Suzuki, K Kakisu, Y Hori - Contact Lens and Anterior Eye, 2025 - Elsevier
  • Purpose To investigate the potential impact of lipid degradation deposition and tear film stability on contact lens discomfort in silicone hydrogel soft contact lens (SCL) wearers. Methods

Development of organotypic human breast cell cultures for high throughput testing

  • https://scholarworks.calstate.edu/downloads/0c483k034
  • A Agarwal - 2014 - scholarworks.calstate.edu
  • Breast cancer is not a disease of individual cells, but a collective failure of cells and tissues to communicate properly. Malignant transformation of breast cells is a complex multi-stage

ANALYTICAL PERFORMANCE OF NOVEL ASSAY FOR MEASURING SEX HORMONE-BINDING GLOBULIN (SHBG) USING FLUORESCENCE IMMUNOASSAY BY

Systemic and mucosal levels of lactoferrin in very low birth weight infants supplemented with bovine lactoferrin

Effects of an Acute Bout of Exercise on Salivary Lactoferrin Responses among Smokers and Non-Smokers

Effect of Aerobic and Arm Exercises on HbA1c and Immunological Properties of Breast Milk in Diabetic Lactating Women

IgG-aggregates rapidly up-regulate FcgRI expression at the surface of human neutrophils in a FcgRII-dependent fashion: A crucial role for FcgRI in the generation of reactive oxygen species

Understanding the aberrant responses of neutrophils in lupus for the development of new therapeutic approaches

Ascitic fluid regulates the local innate immune responseof patients with cirrhosis

Ascitic Fluid Markers Hepcidin, Calprotectin, and Lactoferrin in Early Diagnosis and Follow-up of Spontaneous Bacterial Peritonitis

PREDICTION OF CLINICAL RESPONSE TO IL23-ANTAGONISTS USING IL23 PATHWAY BIOMARKERS

Cardiometabolic risk factors and lactoferrin: Polymorphisms and plasma levels in French-Canadian children

Complement C5a Functions as a Master Switch for the pH Balance in Neutrophils Exerting Fundamental Immunometabolic Effects

Products derived from amniotic fluid and methods of use

A Prospective Study of Iron Status of Exclusively Breastfed Infants Weighing 1800-2499g At Birth and Correlation With Breast Milk Lactoferrin

Complement C5a-induced Functional change in neutrophil granulocytes during systemic inflammation

Oxidant and antioxidant status of human breast milk during lactation period

LACTOFERRIN ACCUMULATION IN FETAL INTESTINE

Lactoferrin fusion protein and method for preparation thereof

Modulation of nasopharyngeal innate defenses by viral coinfection predisposes individuals to experimental pneumococcal carriage

Exercise, But Not Acute Sleep Loss, Increases Salivary Antimicrobial Protein Secretion

International Scholarly Research Notices. Study on the Therapeutic Benefit on Lactoferrin in Patients with Colorectal Cancer Receiving Chemotherapy

The effects of exercise, sex, and menstrual phase on salivary antimicrobial proteins

Separation of Lactoferrin from Human Saliva Using Monolithic Disc

Lactoferrin-Endothelin-1 Axis Contributes to the Development and Invasiveness of Triple-Negative Breast Cancer Phenotypes

Studies on the interaction between IgA, lactoferrin and lysozyme in the breastmilk of lactating women with sick and healthy babies. Lactoferrin and lysozyme

Differential Proteomic Analysis Distinguishes Tissue Repair Biomarker Signatures in Wound Exudates Obtained from Normal Healing and Chronic Wounds

INFLAMMATORY BOWEL DISEASE BIOMARKERS AND RELATED METHODS OF TREATMENT

Iron-Related Proteins: Candidate Urine Biomarkers in Childhood HIV-Associated Renal Diseases

Impact of lipid degradation and tear film stability on silicone hydrogel contact lens wearers with discomfort.

  • https://pubmed.ncbi.nlm.nih.gov/41529586/
  • Takashi Itokawa, Takashi Suzuki, ..., Yuichi Hori (2026). Contact lens & anterior eye : the journal …. 2026 Jun 02
  • The Human Lactoferrin AssayMax ELISA Kit from Assaypro played a significant role in the study by accurately quantifying lactoferrin levels on the contact lenses. This quantification wa…

Association Between Continuous Consumption of Yogurt Fermented with Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1, Salivary Antimicrobial Proteins, and Tongue-Coating Microbiota: An Observational Human Study

  • https://pubmed.ncbi.nlm.nih.gov/41682927/
  • Yuko Yamamoto, Toshiya Morozumi, ..., Alessia Bramanti (2026). Journal of Clinical Medicine. 2026 Feb 04
  • .. analyzed using the Human DEFB-3/Beta-defensin 103/BD-3 ELISA Kit (RayBiotech, Inc., Peachtree Corners, GA, USA).. Lactoferrin concentration was assessed using the AssayMax Human Lactoferrin ELISA Kit (AssayPro LLC., St. Charles, MO, USA).. The lysozyme concentration was measured using the AssayMax Human Lysozyme ELISA Kit (AssayPro LLC., ..

The Impact of Holder Pasteurization on Macronutrients, Vitamins, Minerals, and Bioactive Factors in Human Milk Processed in a Milk Bank Setting.

  • https://pubmed.ncbi.nlm.nih.gov/40746762/
  • Autumn Davis, Sheridan Lee, ..., Maryanne T Perrin (2025). Current Developments in Nutrition. 2025 Aug 01
  • Lactoferrin concentrations were assessed in duplicate at UNCG with a commercial ELISA kit (P/N EL2011-1, AssayPro) per the manufacturer’s instruction.. Kits were acquired from the same lot and batch number, and we used a ..

Effect of kangaroo mother care in low birth weight infants on human milk intake: a randomized controlled trial.

  • https://pubmed.ncbi.nlm.nih.gov/39947481/
  • Bireshwar Sinha, Sarmila Mazumder, ..., Nita Bhandari (2025). The American journal of clinical nutrition. 2025 Feb 11
  • .. conducted in duplicates in single batch.All analysis were conducted in duplicates in single batch.. We used ELISA kits to 154 measure concentration of sIgA (Cat No- E-EL-H1275, Elabscience, US) and lactoferrin (cat # 155 EL2011-1, Assaypro, US) following the manufacturer's protocol.. 156 Sample Size 157 With 95% confidence and 90% power, a sample size of ..

Customizable OpenGUS immunoassay: A homogeneous detection system using β-glucuronidase switch and label-free antibody.

  • https://pubmed.ncbi.nlm.nih.gov/39316870/
  • Bo Zhu, Yukihiko Yamasaki, ..., Tetsuya Kitaguchi (2024). Biosensors & bioelectronics. 2024 Nov 07
  • .. BioVision (USA).Lactoferrin obtained from human milk (catalog No. 7550-2) was purchased from BioVision (USA).. A human lactoferrin ELISA kit (catalog No. EL2011-1) was purchased from Assay Pro (USA).. Colorimetric substrate p-nitrophenyl β-D-glucuronide (PNPG, catalog No. N0618) was purchased from Tokyo Chemical Industry, ..

Point-of-care human milk concentration by passive osmosis: comprehensive analysis of fresh human milk samples.

  • https://pubmed.ncbi.nlm.nih.gov/38760580/
  • Elizabeth R Schinkel, Elizabeth R Nelson, ..., Sarah N Taylor (2024). Journal of perinatology : official journal…. 2024 May 17
  • .. content was 2.2% (range, 0.2–9.3%).The average CV for lactose content was 2.2% (range, 0.2–9.3%).. The lactoferrin concentration was determined in triplicate using a commercial enzyme-linked immunosorbent assay (ELISA) kit for human lactoferrin (Cat. No. EL2011-1, AssayPro, St. Charles, MO, USA) [25].. The average CV for lactoferrin concentration was 3.5% (range, 0.7–11.4%).The average CV for lactoferrin ..

Links Between Vitamin D-Binding Protein, Alpha-1 Antitrypsin and Neutrophil Proteins in Meconium.

  • https://pubmed.ncbi.nlm.nih.gov/36751131/
  • Barbara Lisowska-Myjak, Ewa Skarżyńska, Artur Jakimiuk (2023). Cellular physiology and biochemistry : int…. 2023 Feb 09
  • .. Marburg, Germany • for calprotectin: the PhiCal Calprotectin ELISA Kit (MRP 8/14), Immudiagnostik AG • for myeloperoxidase: the Myeloperoxidase (stool, urine) ELISA kit, DRG Instruments GmbH, Marburg, Germany • for elastase: the Human PMN-elastase ELISA, BioVendor Research and Diagnostic Products • for lactoferrin: The Human Lactoferrin ELISA kit, Assaypro LLC, St. Charles, MO Measurements were performed in duplicate and the mean value calculated was reported as a final result.

The Influence of Lactoferrin in Plasma and Peritoneal Fluid on Iron Metabolism in Women with Endometriosis.

  • https://pubmed.ncbi.nlm.nih.gov/36675136/
  • Ewa Skarżyńska, Monika Wróbel, ..., Piotr Laudański (2023). International Journal of Molecular Science…. 2023 Jan 24
  • Concentrations of LF were measured by enzyme-linked immunosorbent assay (ELISA) using a commercially available AssayMaxTM Human Lactoferrin ELISA Kit (Assaypro LLC–St.. Charles, MO, USA, www.assaypro.com, accessed on 1 January 2020).Charles, MO, USA, www.assaypro.com, accessed on ..

Breast Milk and Saliva Lactoferrin Levels and Postnatal Cytomegalovirus Infection

  • https://pubmed.ncbi.nlm.nih.gov/32069486/
  • Kristin E. D. Weimer, Hunter Roark, ..., Sallie R. Permar (2023). American journal of perinatology. 2023 Jan 19
  • .. blank wells on the same plate in the 1:3 dilution.. Mothers were considered CMV positive if they tested positive in breast milk by CMV gB ELISA, CMV qPCR, or both. . Lactoferrin ELISA: A commercial ELISA kit for human lactoferrin (AssayPro, St. Charles, MO) was used to determine lactoferrin concentration in saliva and breast milk.. The concentration was determined using the provided standard control.The concentration ..

Effects of Heat-Not-Burn Cigarette Smoking on the Secretion of Saliva and Its Innate Immune System Components

  • https://pubmed.ncbi.nlm.nih.gov/36611591/
  • Yukihiro Mori, Mamoru Tanaka, ..., Silvia Caruso (2022). Healthcare. 2022 Dec 31
  • The concentrations of Lac and Lys in saliva were assessed using enzyme-linked immunoassay (ELISA) with the AssayMax Human Lactoferrin ELISA kit (AssayPro, St. Charles, MO, USA) and AssayMax Lysozyme ELISA kit (AssayPro), respectively, according to the manufacturer’s instructions [ , ].. In this study, the secretion rates (μg/min) of Lac and ..

Effects of Long-Term Supplementation of Bovine Colostrum on Iron Homeostasis, Oxidative Stress, and Inflammation in Female Athletes: A Placebo-Controlled Clinical Trial

  • https://pubmed.ncbi.nlm.nih.gov/36615842/
  • Mirosława Cieślicka, Joanna Ostapiuk-Karolczuk, ..., Rosa Casas (2022). Nutrients. 2022 Dec 30
  • .. SOD (SOD Activity Assay Kit, SunRed): TBARS (Human Thiobarbituric Acid Reactive Substance ELISA Kit; SunRed), PC (Human Protein Carbonyl ELISA Kit; SunRed), TAC (Total Antioxidant Capacity ELISA Kit; Omnignostica Forschungs GmbH), IL-6 (Human Interleukin 6 ELISA Kit; SunRed), Hepcidin 25 (DRG Hepcidin 25 ELISA Kit), ferritin (Human Ferritin ELISA Kit; DRG), transferrin (Transferrin Human ELISA Kit; SunRed), HPX (Human Hemopexin ELISA Kit; SunRed), Lactoferrin (Human Lactoferrin AssayMax ELISA Kit; AssayPro), and myoglobin (Myoglobin ELISA-DRG).

Systemic and mucosal levels of lactoferrin in very low birth weight infants supplemented with bovine lactoferrin.

  • https://pubmed.ncbi.nlm.nih.gov/32841570/
  • Hannah L Itell, Andrew Berenz, ..., David A Kaufman (2021). Biochemistry and cell biology = Biochimie …. 2021 Aug 27
  • The Human Lactoferrin AssayMax ELISA Kit from Assaypro was integral to measuring lactoferrin levels in very low birth weight infants receiving bovine lactoferrin supplementation. This …

Albumin fusion at the N-terminus or C-terminus of human lactoferrin leads to improved pharmacokinetics and anti-proliferative effects on cancer cell lines.

  • https://pubmed.ncbi.nlm.nih.gov/32946958/
  • Keisuke Ueda, Maya Shimizu, ..., Atsushi Sato (2021). European journal of pharmaceutical science…. 2021 Jun 21
  • In this study, the Human Lactoferrin AssayMax ELISA Kit from Assaypro was utilized to accurately measure plasma concentrations of human lactoferrin (hLF), essential for evaluating the …

Metabo-tip: a metabolomics platform for lifestyle monitoring supporting the development of novel strategies in predictive, preventive and personalised medicine

  • https://pubmed.ncbi.nlm.nih.gov/34188726/
  • Julia Brunmair, Andrea Bileck, ..., Christopher Gerner (2021). The EPMA Journal. 2021 May 04
  • The Human Lactoferrin AssayMax ELISA Kit from Assaypro played a crucial role in the study by enabling an in-depth analysis of molecular profiling related to the influences of bioactive…

A Cluster Analysis of Bronchiectasis Patients Based on the Airway Immune Profile

  • https://pubmed.ncbi.nlm.nih.gov/33217421/
  • Lídia Perea, Elisabet Cantó, ..., Silvia Vidal (2021). Chest. 2021 May 01
  • Abstract: Clinical heterogeneity in bronchiectasis remains a challenge for improving the appropriate targeting of therapies and patient management. Antimicrobial peptides (AMPs) have been linked to di…

Psychobiotic Supplementation of PS128TM Improves Stress, Anxiety, and Insomnia in Highly Stressed Information Technology Specialists: A Pilot Study

  • https://pubmed.ncbi.nlm.nih.gov/33842519/
  • Shu-I Wu, Chien-Chen Wu, ..., Ying-Chieh Tsai (2021). Frontiers In Nutrition. 2021 Mar 26
  • ne fatigue subjects ( 53 ). Salivary α-amylase, IgA, lactoferrin, and lysozyme levels were measured by enzyme-linked immunosorbent assay kits (Immuno-Biological Laboratories, Inc., USA; Germany; and Assaypro LLC, USA). Salivary cortisol levels were determined using an electrochemiluminescence immunoassay kit (Elecsys Cortisol, Roche Diagnostic, Germany). All procedures were performed according to

Can aminopeptidase N determined in the meconium be a candidate for biomarker of fetal intrauterine environment?

  • https://pubmed.ncbi.nlm.nih.gov/32360796/
  • Barbara Lisowska-Myjak, Paulina Wilczyńska, ..., Ewa Skarżyńska (2020). Experimental and molecular pathology. 2020 Oct 29
  • In the study investigating aminopeptidase N/CD13 as a potential biomarker for fetal intrauterine environment stress, the Human Lactoferrin AssayMax ELISA Kit from Assaypro played a sig…

IgG‐aggregates rapidly upregulate FcgRI expression at the surface of human neutrophils in a FcgRII‐dependent fashion: A crucial role for FcgRI in the generation of reactive oxygen species

  • https://pubmed.ncbi.nlm.nih.gov/32946139/
  • Sandrine Huot, Cynthia Laflamme, ..., Marc Pouliot (2020). The FASEB Journal. 2020 Sep 18
  • .. Bio Basic (Markham, ON, Canada).Cytochrome C was purchased from Bio Basic (Markham, ON, Canada).. Human MPO and human Lactoferrin ELISA kits were purchased from Assaypro (St. -- 3HUOT eT al. Charles, MO, USA).. MMP-9 DuoSet ELISA was purchased from R&D Systems, Inc (Oakville, ON, Canada).MMP-9 DuoSet ELISA ..

Optimized lactoferrin as a highly promising treatment for intracerebral hemorrhage: Pre-clinical experience

  • https://pubmed.ncbi.nlm.nih.gov/32438861/
  • Xiurong Zhao, Marian Kruzel, ..., Jaroslaw Aronowski (2020). Journal of Cerebral Blood Flow & Metabolis…. 2020 May 21
  • Abstract: Intracerebral hemorrhage (ICH) is the deadliest form of stroke for which there is no effective treatment, despite an endless number of pre-clinical studies and clinical trials. The obvious t…

Randomized Controlled Trial of Bovine Lactoferrin for Prevention of Sepsis and Neurodevelopment Impairment in Infants

  • https://pubmed.ncbi.nlm.nih.gov/32037149/
  • Theresa J Ochoa, Jaime Zegarra, ..., Linda Ewing-Cobbs (2020). The Journal of pediatrics. 2020 Apr 10
  • was mother’s own fresh expressed milk; no donor milk or probiotics were used. Breast milk (2–3mL) was collected in the first 7d of life (colostrum) and at 1 month to measure human-LF using an ELISA (Assaypro, St.Charles, MO, USA). We followed infants up to 24 months corrected age by phone every 2 weeks. Pediatric evaluations were performed at 3, 6, 12, 18 and 24 months; auditory brainstem respons

Is mother’s own milk lactoferrin intake associated with less neonatal sepsis, NEC or death?

  • https://pubmed.ncbi.nlm.nih.gov/32053823/
  • Theresa J Ochoa, Karina Mendoza, ..., Veerle Cossey (2020). Neonatology. 2020 Feb 21
  • in Lima. We requested a breastmilk sample in the first 6 days of life (colostrum) and between 7 and 14 days (transitional milk) to measure the concentration of human-LF using a commercial ELISA kit (Assaypro, St.Charles, MO, USA) [ 18 ]. Study definitions: culture-proven LOS, clinical signs and symptoms of infection and a positive blood culture obtained after 72h of life; probable sepsis or cultu

Relationships between meconium concentrations of acute phase proteins.

  • https://pubmed.ncbi.nlm.nih.gov/29908081/
  • Barbara Lisowska-Myjak, Ewa Skarżyńska, ..., Artur Jakimiuk (2019). Clinical and experimental pharmacology & p…. 2019 Sep 30
  • In this study, the Human Lactoferrin AssayMax ELISA Kit from Assaypro played a significant role in measuring lactoferrin concentrations in meconium samples. This measurement was essent…

Antimicrobial peptides, disease severity and exacerbations in bronchiectasis

  • https://pubmed.ncbi.nlm.nih.gov/32273337/
  • Oriol Sibila, Lídia Perea, ..., James D Chalmers (2019). Thorax. 2019 Jul 05
  • 50 000 g for 90 min followed by careful extraction of the supernatant. Samples were processed within 2 hours of expectoration and immediately frozen at −80°C. Serum and sputum lactoferrin, lysozyme (AssayPro, St. Charles, Missouri, USA), LL-37 (Hycult Biotech, Plymouth, Pennsylvania, USA), SLPI and matrix metallopeptidase 9 (MMP-9) (R Systems, Minneapolis, Minnesota, USA) levels were measured by v

Antidiabetic efficacy of lactoferrin in type 2 diabetic pediatrics; controlling impact on PPAR-γ, SIRT-1, and TLR4 downstream signaling pathway

  • https://pubmed.ncbi.nlm.nih.gov/30534206/
  • Waleed A. Mohamed, Mona F. Schaalan (2018). Diabetology & metabolic syndrome. 2018 Dec 04
  • Plasma Lf concentration was assessed in plasma using an ELISA kit (Assaypro EL2011-1, St.-Charles, USA) that is highly specific to human Lactoferrin.. This assay employed a quantitative sandwich enzyme immunoassay technique, which measured LF in 5 ..

Ascitic fluid regulates the local innate immune response of patients with cirrhosis

  • https://pubmed.ncbi.nlm.nih.gov/29851144/
  • Juan C Nieto, Lídia Perea, ..., Sílvia Vidal (2018). Journal of leukocyte biology. 2018 May 31
  • Abstract: Ascitic neutrophils from cirrhotic patients with spontaneous bacterial peritonitis (SBP) exhibit an impaired oxidative burst that could facilitate bacterial infection. However, the influence…

Hinge-Deficient IgG1 Fc Fusion: Application to Human Lactoferrin.

  • https://pubmed.ncbi.nlm.nih.gov/28763236/
  • Yuki Shiga, Daisuke Murata, ..., Atsushi Sato (2018). Molecular Pharmaceutics. 2018 May 14
  • In the study, the Human Lactoferrin AssayMax ELISA Kit from Assaypro was instrumental in measuring the plasma levels of the engineered hLF-CH2-CH3 fusion protein following its administ…

Correlations between ceruloplasmin, lactoferrin and myeloperoxidase in meconium.

  • https://pubmed.ncbi.nlm.nih.gov/27903408/
  • Ewa Skarżyńska, Joanna Żytyńska-Daniluk, Barbara Lisowska-Myjak (2018). Journal of trace elements in medicine and …. 2018 Apr 17
  • In this study, the Human Lactoferrin AssayMax ELISA Kit from Assaypro played a crucial role in quantifying lactoferrin concentrations in meconium samples from healthy neonates. This re…

Variations in serum concentrations of C-reactive protein, ceruloplasmin, lactoferrin and myeloperoxidase and their interactions during normal human pregnancy and postpartum period

  • https://pubmed.ncbi.nlm.nih.gov/29413114/
  • Ewa Skarżyńska, Hanna Zborowska (2018). Journal of Trace Elements in Medicine and …. 2018 Mar 01
  • Abstract: Serum proteins may provide information about homeostasis of redox status and inflammatory processes also during pregnancy. The aim of the study was to assess the dynamics of changes in serum…

Correlation between the concentrations of lactoferrin and neutrophil gelatinase-associated lipocalin in meconium

  • https://pubmed.ncbi.nlm.nih.gov/29285663/
  • Barbara Lisowska-Myjak, Ewa Skarżyńska, ..., Artur Jakimiuk (2017). Biometals. 2017 Dec 29
  • Measurements of meconiun protein concentrations were performed according to the manufacturer’s instructions in a MINIBOS immunochemistry analyser (DiaSorin), using the following kits: for lactoferrin The Human Lactoferrin ELISA kit, Assaypro LLC for NGAL The NGAL ELISA kit, BioPorto Diagnostics Concentrations of lactoferrin expressed as ng/ml homogenate were calculated as µg/g meconium.

Concentrations of neutrophil-derived proteins in meconium and their correlations.

  • https://pubmed.ncbi.nlm.nih.gov/27414433/
  • Barbara Lisowska-Myjak, Joanna Żytyńska-Daniluk, Ewa Skarżyńska (2017). Biomarkers in Medicine. 2017 Oct 25
  • In this study, the Human Lactoferrin AssayMax ELISA Kit from Assaypro played a crucial role by allowing for the specific measurement of lactoferrin levels in meconium samples from neon…

Complement C5a Functions as a Master Switch for the pH Balance in Neutrophils Exerting Fundamental Immunometabolic Effects.

  • https://pubmed.ncbi.nlm.nih.gov/28490576/
  • Stephanie Denk, Miriam D Neher, ..., Markus S Huber-Lang (2017). Journal of Immunology (Baltimore, Md. : 19…. 2017 Sep 27
  • .. was determined using a spectrophotometric reader at 450 nm (CytoFluor Series 4000; Perspective Biosystems).. Concentrations of lactoferrin in neutrophil supernatants were measured using a commercially available ELISA kit, specific for human lactoferrin, according to the manufacturer’s instructions (Assaypro).

Cardiometabolic risk factors and lactoferrin: polymorphisms and plasma levels in French-Canadian children

  • https://pubmed.ncbi.nlm.nih.gov/28678770/
  • Valérie Marcil, Sylvain Mayeur, ..., Emile Levy (2017). Pediatric research. 2017 Aug 16
  • an Coulter, Inc.), which has no cross-reactivity with proinsulin or C-peptide. Plasma FFA concentrations were quantified by an enzymatic colorimetric method (Wako Chemicals). Plasma LTF was measured using a commercial ELISA kit (AssayPro, St. Charles MO) in a sampling of 176 children aged 9, 13 and 16 years old selected according to their extreme HDL-C values in order to detect the largest effect.

Salivary Immune and Metabolic Marker Analysis (SIMMA): A Diagnostic Test to Predict Caries Risk

  • https://pubmed.ncbi.nlm.nih.gov/28654016/
  • Alex Mira, Alejandro Artacho, ..., Aurea Simon-Soro (2017). Diagnostics. 2017 Jun 27
  • In the study examining caries risk, the Human Lactoferrin AssayMax ELISA Kit from Assaypro played a vital role in measuring lactoferrin levels in saliva, which was essential for determ…

Meconium lactoferrin levels in neonates: can we predefine normal values?

  • https://pubmed.ncbi.nlm.nih.gov/27017926/
  • Ewa Skarżyńska, Joanna Żytyńska-Daniluk, Barbara Lisowska-Myjak (2017). The journal of maternal-fetal & neonatal m…. 2017 May 24
  • In the study, the Human Lactoferrin AssayMax ELISA Kit from Assaypro played an essential role in quantifying lactoferrin concentrations in neonatal meconium. Utilizing this kit allowed…

Lactoferrin Concentration in Breast Milk of Mothers of Low-Birth-Weight Newborns

  • https://pubmed.ncbi.nlm.nih.gov/28125095/
  • C G Turin, A Zea-Vera, ..., (2017). Journal of perinatology : official journal…. 2017 May 10
  • then, the fat layer was removed and the milk serum was kept frozen at −70°C until LF quantification. We measured the LF concentration using a commercial enzyme-linked immunosorbent assay kit (ELISA) (Assaypro LLC, 30 Triad South Drive St., Charles, MO 63304, USA) according to the manufacturer's instructions. All the reagents were allowed to be warmed up to room temperature (25°C) before use. The pl

High-temperature short-time pasteurisation of human breastmilk is efficient in retaining protein and reducing the bacterial count

  • https://pubmed.ncbi.nlm.nih.gov/28135766/
  • Daniel Klotz, Mirjam Joellenbeck (2017). Acta Paediatrica. 2017 Feb 28
  • Abstract: Milk banks are advised to use Holder pasteurisation to inactivate the cytomegalovirus, but the process adversely affects the bioactive properties of human breastmilk. This study explored the…

Relationship between salivary immunoglobulin a, lactoferrin and lysozyme flow rates and lifestyle factors in Japanese children: a cross-sectional study.

  • https://pubmed.ncbi.nlm.nih.gov/27565888/
  • Momo Ide, Juri Saruta, ..., Keiichi Tsukinoki (2017). Acta odontologica Scandinavica. 2017 Jan 17
  • Salivary lactoferrin concentrations were measured using a Human Lactoferrin ELISA Kit (Assaypro LLC, St. Charles, MO).. Briefly, sample and human lactoferrin standards (Assaypro LLC) were added to each well of ..

A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking) A longitudinal study of human milk composition in the second year postpartum: implications for human milk banking

  • https://pubmed.ncbi.nlm.nih.gov/26776058/
  • Maryanne T Perrin, April D Fogleman, ..., Jonathan C Allen (2017). Maternal & Child Nutrition. 2017 Jan 15
  • ouri USA) bacterial suspension (Shugar 1952 ), and was adapted to a 96‐well plate format (Meng 2014 ). Lactoferrin and IgA were measured using an ELISA assay (EL2011‐1 and EI7001‐1, respectively; AssayPro, St. Charles, Missouri USA). The average intra‐assay coefficient of variation (CV) was 1.6%, 3.7%, 3.8% and 4.0% for total protein, lysozyme, lactoferrin and IgA, respectively. All protein ass

Plasma Lactoferrin Levels Positively Correlate with Insulin Resistance despite an Inverse Association with Total Adiposity in Lean and Severely Obese Patients

  • https://pubmed.ncbi.nlm.nih.gov/27902700/
  • Sylvain Mayeur, Alain Veilleux, ..., Andrew Wolfe (2016). PLoS ONE. 2016 Nov 30
  • Plasma Lf concentrations were determined in plasma (after a night of fasting) using an ELISA kit (Assaypro EL2011-1, St.-Charles, USA) that is highly specific to human Lf.. The intra- and inter-assay coefficients of variation were 4.8 and 7.3%, respectively.The intra- and ..

Lactoferrin conjugated iron oxide nanoparticles for targeting brain glioma cells in magnetic particle imaging

  • https://pubmed.ncbi.nlm.nih.gov/26412614/
  • Asahi Tomitaka, Hamed Arami, ..., Kannan M. Krishnan (2016). Nanoscale. 2016 Oct 28
  • The experiments were performed in quadruplicate.The experiments were performed in quadruplicate.. The binding affinity of lactoferrin conjugated to the surface of iron oxide nanoparticles was determined by the AssayMax Human Lactoferrin ELISA Kit (Assaypro) according to the manufacturer's protocols.. A standard curve was prepared with free lactoferrin to compare the binding affinity of ..

The effect of nasal irrigation formulation on the antimicrobial activity of nasal secretions.

  • https://pubmed.ncbi.nlm.nih.gov/26205877/
  • Charmaine M Woods, Sophia Tan, ..., A Simon Carney (2016). International forum of allergy & rhinology. 2016 Oct 19
  • In the study, the Human Lactoferrin AssayMax ELISA Kit from Assaypro was employed to quantify lactoferrin levels in nasal secretions, which was crucial for estimating antimicrobial pep…

Study on the Therapeutic Benefit on Lactoferrin in Patients with Colorectal Cancer Receiving Chemotherapy.

  • https://pubmed.ncbi.nlm.nih.gov/27350986/
  • Tarek M Moastafa, Alaa El-Din Elsayed El-Sissy, ..., Mai Salah El-Din Koura (2016). International Scholarly Research Notices. 2016 Jun 28
  • .. of (BUN), serum creatinine, aspartate aminotransferase (AST), and alanine aminotransferase (ALT), all of which were analyzed spectrophotometry using Beckman Synchron CX9 Pro Chemistry Analyzer (Beckman kits, Beckman, California, United States), serum LF by ELISA using TECAN ELISA reader (ELISA kit Assaypro Company, Saint Charles, United States, Catalog Number EL2011-1), serum GST enzyme by ELISA using TECAN ELISA reader (ELISA kit Cusabio, United States, Company Catalog Number: CSB-E09032h), and INF by ELISA using TECAN ELISA reader (ELISA kit ..

Recombinant human lactoferrin-Fc fusion with an improved plasma half-life.

  • https://pubmed.ncbi.nlm.nih.gov/25433245/
  • Yuki Shiga, Yuta Oshima, ..., Atsushi Sato (2015). European journal of pharmaceutical science…. 2015 Aug 31
  • In the study, the Human Lactoferrin AssayMax ELISA Kit from Assaypro was utilized to quantitatively measure the concentration of hLF in rat blood post-injection of hLF-hinge-CH2-CH3. T…

Exercise, But Not Acute Sleep Loss, Increases Salivary Antimicrobial Protein Secretion

  • https://pubmed.ncbi.nlm.nih.gov/25915527/
  • Trevor L. Gillum, Matthew R. Kuennen, ..., Alex T. Jordan-Patterson (2015). Journal of Strength and Conditioning Resea…. 2015 May 01
  • Abstract: Sleep deficiencies may play a role in depressing immune parameters. Little is known about the impact of exercise after sleep deprivation on mucosal immunity. The purpose of this study was to…

Interplay between Bladder Microbiota and Urinary Antimicrobial Peptides: Mechanisms for Human Urinary Tract Infection Risk and Symptom Severity

  • https://pubmed.ncbi.nlm.nih.gov/25486068/
  • Vanessa Nienhouse, Xiang Gao, ..., R. Mark Wooten (2014). PLoS ONE. 2014 Dec 08
  • AMP concentrations were measured by ELISA; human β-defensin-1 (PeproTech, Cat # 900-K202), human β-defensin-2 (PeproTech, Cat # 900-K172), psoriasin (Circulex, Cat # CY-8073) and lactoferrin (AssayPro, Cat # EL2011-1).. Protease activity was assessed with an Enzchek protease assay (Invitrogen, Cat # E6638).Protease activity ..

SALIVARY ANTIMICROBIAL PROTEIN RESPONSE TO PROLONGED RUNNING

  • https://pubmed.ncbi.nlm.nih.gov/24744458/
  • T.L. Gillum, M. Kuennen, ..., P. Moseley (2013). Biology of Sport. 2013 Jan 21
  • ression osmometer (Advanced Instruments, Norwood, MA, USA) after calibration with 290 mOsm/kg NaCl solution. Saliva was then analyzed using ELISA according to manufacturer's instruction. Lys and Lac (AssayPro, St. Charles, MO, USA) was detectable at 0.1 ng · ml-1 with an intra-assay coefficient of 4.1% and an inter-assay coefficient of 7.2%. IgA (Salimetrics, State College, PA, USA) was detectible

The dominant expression of functional human lactoferrin in transgenic cloned goats using a hybrid lactoferrin expression construct.

  • https://pubmed.ncbi.nlm.nih.gov/22789477/
  • Huiqing Yu, Jianquan Chen, ..., Guoxiang Cheng (2013). Journal of biotechnology. 2013 Jan 09
  • In the study, the Human Lactoferrin AssayMax ELISA Kit from Assaypro was instrumental in measuring the levels of recombinant human lactoferrin (rhLF) in the milk of transgenic cloned g…

Immune Defense against S. Epidermidis Biofilms: Components of the Extracellular Polymeric Substance Activate Distinct Bactericidal Mechanisms of Phagocytic Cells

  • https://pubmed.ncbi.nlm.nih.gov/23065886/
  • Eva Meyle, Gerald Brenner-Weiss, ..., G. Maria Hänsch (2012). The International Journal of Artificial Or…. 2012 Oct 01
  • In the study, the Human Lactoferrin AssayMax ELISA Kit from Assaypro was pivotal in identifying and quantifying lactoferrin levels within Staphylococcus epidermidis biofilms. This capa…

The role of fecal calprotectin and lactoferrin in the diagnosis of necrotizing enterocolitis

  • https://pubmed.ncbi.nlm.nih.gov/22079952/
  • Mukadder Ayşe Selimoğlu, İsmail Temel (2012). Pediatric Critical Care Medicine. 2012 Jul 01
  • Abstract: Early detection of necrotizing enterocolitis can improve the prognosis, however, there is not a reliable laboratory test to detect either newborns at risk for necrotizing enterocolitis devel…

Establishing Human Lacrimal Gland Cultures with Secretory Function

  • https://pubmed.ncbi.nlm.nih.gov/22253725/
  • Shubha Tiwari, Mohammad Javed Ali, ..., Songtao Shi (2012). PLoS ONE. 2012 Jan 13
  • .. ..488 (Invitrogen, Carlsbad, CA, USA), Phycoerytherin (eBioscience), human lactoferrin ELISA kit (AssayPro, MO, USA), human lysozyme ELISA kit (AssayPro, MO, USA),.. ..

Lactoferrin–Endothelin-1 Axis Contributes to the Development and Invasiveness of Triple-Negative Breast Cancer Phenotypes

  • https://pubmed.ncbi.nlm.nih.gov/22006997/
  • Ngoc-Han Ha, Vasudha S. Nair, ..., Rakesh Kumar (2011). Cancer Research. 2011 Oct 17
  • .. Limited).The final images were developed using the Storm 865 Imager (GE Healthcare UK Limited).. Plasma protein detection using ELISA For detection of lactoferrin, the AssayMax Human Lactoferrin ELISA Kit (AssayPro) was used with a 1:100 dilution of serum samples.. ET-1 levels were measured using the QuantiGlo Human Endothelin-1 Kit (R&D Systems) by following ..

The relationship of fetuin-A and lactoferrin with bone mass in elderly women

  • https://pubmed.ncbi.nlm.nih.gov/20963400/
  • L. Chailurkit, A. Kruavit, ..., B. Ongphiphadhanakul (2010). Osteoporosis International. 2010 Oct 21
  • In the study investigating bone-related phenotypes in elderly women, the Human Lactoferrin AssayMax ELISA Kit from Assaypro was utilized to assess serum lactoferrin levels. This kit pl…

Differential Proteomic Analysis Distinguishes Tissue Repair Biomarker Signatures in Wound Exudates Obtained from Normal Healing and Chronic Wounds

  • https://pubmed.ncbi.nlm.nih.gov/20666496/
  • Sabine A. Eming, Manuel Koch, ..., Jay W. Fox (2010). Journal of Proteome Research. 2010 Sep 03
  • In the study, the Human Lactoferrin AssayMax ELISA Kit from Assaypro was employed to quantify lactoferrin levels in wound exudates, which was essential for distinguishing between heali…

Iron-Related Proteins: Candidate Urine Biomarkers in Childhood HIV–Associated Renal Diseases

  • https://pubmed.ncbi.nlm.nih.gov/19279121/
  • Ángel A. Soler-García, Douglas Johnson, ..., Patricio E. Ray (2010). Clinical Journal of the American Society o…. 2010 Apr 01
  • n was determined by ferrozine staining as described previously ( 14 ). Haptoglobin, hemopexin, transferrin, lactoferrin, and neutrophil gelatinase-associated lipocalin (NGAL) were measured by ELISA (Assay Pro, St. Charles, MO; and Assay Designs, Inc., Ann Arbor, MI; respectively). The presence of iron in the kidney of HIV-1 infected children and HV-Tg rats was detected by Prussian blue staining us

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