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Mouse Leptin ELISA Kit

Original price was: $438.00.Current price is: $395.00.

Catalog Number: EML2001-1

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
Product Size:96 Well Plate

Catalog Number. EML2001-1 Categories: , , Tag: Brand:

Additional information

Format

Sandwich ELISA

Entrez Gene

16846

Components

Biotinylated Mouse Leptin Antibody, Chromogen Substrate (1x), MIX Diluent Concentrate (10x), Mouse Leptin Microplate, Mouse Leptin Standard, Sealing Tapes, SP Conjugate (100x), Standard Diluent (1x), Stop Solution (1x), Wash Buffer Concentrate (20x)

TIme

4 hours

Sensitivity

0.35 ng/ml

Range

0.75 – 48 ng/ml

Samples Type

Cell Culture Supernatants, Plasma, Serum

Associated Disease

Diabetes

Species

Mouse

UniProt

P41160

Storage

Refer to component labels for details

Plex

1

Usage

For Research Use Only, Not To Be Used For Diagnostic Purposes

Documentation

Publications citing Assaypro: Mouse Leptin ELISA Kit... (32 results)

Please use discretion when following external links; Assaypro cannot guarantee the validity and security of all citation links provided.

Macrophage CD40 plays a minor role in obesity-induced metabolic dysfunction

Identification of a Lactobacillus plantarum strain that ameliorates chronic inflammation and metabolic disorders in obese and type 2 diabetic mice

  • https://www.sciencedirect.com/science/article/pii/S0022030215008851
  • T Toshimitsu, J Mochizuki, S Ikegami, H Itou - Journal of dairy science, 2016 - Elsevier
  • In this study, we identified a strain of lactic acid bacteria (LAB) that induces high levels of IL-10 production by immune cells, and evaluated the ability of the strain to suppress chronic

A new hope for obesity management: Boron inhibits adipogenesis in progenitor cells through the Wnt/?-catenin pathway

Depletion of CD40 on CD11c+ cells worsens the metabolic syndrome and ameliorates hepatic inflammation during NASH

  • https://www.nature.com/articles/s41598-019-50976-6
  • S Aarts, M Reiche, M den Toom, M Gijbels, L Beckers - Scientific reports, 2019 - nature.com
  • Plasma leptin and adiponectin levels of the DIO mice were measured using a mouse leptin ELISA Kit (ChrystalChem) and a mouse adiponectin ELISA Kit (AssayPro). Liver enzymes

Bacteroides uniformis CECT 7771 alleviates inflammation within the gut-adipose tissue axis involving TLR5 signaling in obese mice

  • https://www.nature.com/articles/s41598-021-90888-y
  • E Fabersani, K Portune, I Campillo, I Lpez-Almela - Scientific reports, 2021 - nature.com
  • This study investigated the immune mechanisms whereby administration of Bacteroides uniformis CECT 7771 reduces metabolic dysfunction in obesity. C57BL/6 adult male mice were

Adipose extracellular matrix deposition is an indicator of obesity and metabolic disorders

Sodium pentaborate pentahydrate ameliorates lipid accumulation and pathological damage caused by high fat diet induced obesity in BALB/c mice

Cactus pear (Opuntia ficus-indica) juice fermented with autochthonous Lactobacillus plantarum S-811

  • https://pubs.rsc.org/en/content/articlehtml/2019/fo/c8fo01591k
  • HE Vern, PG Cano, E Fabersani, Y Sanz, MI Isla - Food & Function, 2019 - pubs.rsc.org
  • The present study aimed at investigating the lactic fermentation of cactus pear (Opuntia ficus-indica) fruit juice with the autochthonous and potentially probiotic strain Lactobacillus

Bezafibrate regulates the expression and enzyme activity of 11?-hydroxysteroid dehydrogenase type 1 in murine adipose tissue and 3T3-L1 adipocytes

  • https://journals.physiology.org/doi/abs/10.1152/ajpendo.00340.2006
  • S Nakano, Y Inada, H Masuzaki - American Journal , 2007 - journals.physiology.org
  • A clinically employed antihyperlipidemic drug, bezafibrate, has been characterized as a PPAR(?, -?, and -?) pan-agonist in vitro. Recent extended trials have highlighted its antidiabetic

Effect of maternal obesity on estrous cyclicity, embryo development and blastocyst gene expression in a mouse model

Bacteroides uniformis CECT 7771 alleviates inflammation within the gut-adipose tissue axis involving TLR5 signaling in obese mice

  • https://ri.conicet.gov.ar/handle/11336/161554
  • ME Fabersani Marrades, K Portune, I Campillo - 2021 - ri.conicet.gov.ar
  • This study investigated the immune mechanisms whereby administration of Bacteroides uniformis CECT 7771 reduces metabolic dysfunction in obesity. C57BL/6 adult male mice were

Evaluation of oral antiretroviral drugs in mice with metabolic and neurologic complications

Impaired metabolic and hepatic functions following subcutaneous lipectomy in adult obese rats

Hepatocyte-specific fibroblast growth factor 21 overexpression ameliorates high-fat diet-induced obesity and liver steatosis in mice

Bifidobacterium pseudocatenulatum CECT 7765 supplementation restores altered vascular function in an experimental model of obese mice

Identification of a Lactobacillus plantarum strain that ameliorates chronic inflammation and metabolic disorders in obese and type 2 diabetic mice

Hepatocyte-specific fibroblast growth factor 21 overexpression ameliorates high-fat diet-induced obesity and liver steatosis in mice.

  • https://pubmed.ncbi.nlm.nih.gov/34732847/
  • Kota Yano, Kanji Yamaguchi, ..., Yoshito Itoh (2022). Laboratory investigation; a journal of tec…. 2022 Mar 14
  • In the study examining the effects of hepatocyte-specific FGF21 overexpression on high-fat diet-induced obesity, the Mouse Leptin AssayMax ELISA Kit from Assaypro was utilized to measu…

Sodium Pentaborate Pentahydrate ameliorates lipid accumulation and pathological damage caused by high fat diet induced obesity in BALB/c mice

  • https://pubmed.ncbi.nlm.nih.gov/33711700/
  • Hüseyin Abdik, Alev Cumbul (2021). Journal of Trace Elements in Medicine and …. 2021 Jul 01
  • Abstract: Obesity is one of the most popular topic in the field of research. In order to defeat this highly widespread disease, the mechanism of fat accumulation at the molecular level and its elimina…

Bacteroides uniformis CECT 7771 alleviates inflammation within the gut-adipose tissue axis involving TLR5 signaling in obese mice

  • https://pubmed.ncbi.nlm.nih.gov/34083551/
  • Emanuel Fabersani, Kevin Portune (2021). Scientific Reports. 2021 Jun 03
  • .. pH 7.4) and kept at 4 °C until further processing for flow cytometry analysis.. Quantification of endocrine and metabolic parametersPlasma leptin concentration was determined by the Assay Max Mouse Leptin ELISA kit (ASSAYPRO, Missouri, USA) with a sensitivity threshold of 0.3 ng/mL.. Insulin was measured using a Rat/Mouse ELISA kit (Sigma, Sant Louis, USA) with a ..

Paternal multigenerational exposure to an obesogenic diet drives epigenetic predisposition to metabolic diseases in mice

  • https://pubmed.ncbi.nlm.nih.gov/33783350/
  • GeorgesGeorges Raad Raad, FabrizioFabrizio Serra Serra, ..., David E James (2021). eLife. 2021 Mar 30
  • stored at −80°C. Serum CRP, leptin, adiponectin, and cholesterol levels were measured with the C-Reactive Protein ELISA (Mouse CRP, Elabscience, CliniSciences S.A.S., Nanterre, France), Leptin ELISA (ASSAYPRO, CliniSciences S.A.S., Nanterre, France), Adiponectin ELISA (mouse Adiponectin, EZMADP-60K, EMD Millipore Corporation, Darmsbalt, Germany), and Cholesterol Assay (Abcam, Paris, France) kits, r

Cactus pear (Opuntia ficus-indica) juice fermented with autochthonous Lactobacillus plantarum S-811.

  • https://pubmed.ncbi.nlm.nih.gov/30720817/
  • Hernán E Verón, Paola Gauffin Cano, ..., Sebastian Torres (2019). Food & Function. 2019 Jun 14
  • In the study investigating the effects of fermented cactus pear juice, the Mouse Leptin AssayMax ELISA Kit from Assaypro played a significant role by enabling the determination of plas…

Evaluation of Oral Antiretroviral Drugs in Mice With Metabolic and Neurologic Complications

  • https://pubmed.ncbi.nlm.nih.gov/30233379/
  • Fuu-Jen Tsai, Mao-Wang Ho (2018). Frontiers in Pharmacology. 2018 Sep 04
  • Blood was collected by cardiac puncture of terminally anesthetized mice at day 31.. The serum levels of leptin ( Figure ; EML2001-1; Mouse Leptin ELISA Kit) and adiponectin ( Figure ; EMA2500-1; Mouse Adiponectin ELISA Kit) were also evaluated by ELISA according to the manufacturer’s protocol (Assaypro, MO, United States).. The serum levels of total cholesterol, HDL-cholesterol, and triglycerides were measured colorimetrically using commercially ..

Bifidobacterium pseudocatenulatum CECT 7765 Ameliorates Neuroendocrine Alterations Associated with an Exaggerated Stress Response and Anhedonia in Obese Mice

  • https://pubmed.ncbi.nlm.nih.gov/28921462/
  • Ana Agusti, A. Moya-Pérez (2017). Molecular neurobiology. 2017 Sep 18
  • Abstract: Obesity, besides being a problem of metabolic dysfunction, constitutes a risk factor for psychological disorders. Experimental models of diet-induced obesity have revealed that obese animals…

A new hope for obesity management: Boron inhibits adipogenesis in progenitor cells through the Wnt/β-catenin pathway

  • https://pubmed.ncbi.nlm.nih.gov/28285642/
  • Ayşegül Doğan, Selami Demirci (2017). Metabolism. 2017 Apr 01
  • .. and band intensitieswere quantified by Image Lab Software, version 5.2.1.. Adiponectin (EMA2500–1, Assaypro, St Charles, MO) and leptin (EML2001–1, Assaypro, St Charles, MO) concentrations in conditioned medium of 3T3-L1 cells were measured using commercially available enzyme-linked immunosorbent assay (ELISA) kits following manufacturer's recommendations.. Lipolysis was evaluated in differentiated cells treated with the specified ..

Bifidobacterium pseudocatenulatum CECT 7765 supplementation restores altered vascular function in an experimental model of obese mice

  • https://pubmed.ncbi.nlm.nih.gov/28539820/
  • María D. Mauricio, Eva Serna (2017). International Journal of Medical Sciences. 2017 Jan 01
  • Determination of serum leptin and insulin concentration Serum leptin concentration was determined by the Assay Max Mouse Leptin ELISA kit (Assay pro, LLC; Ireland) with a sensitivity threshold of 0.3 ng/ml and insulin was measured using a Rat/ Mouse ELISA kit (Merck Millipore, Germany) with a sensitivity threshold of 0.2 ng/ml, according to the manufacturer’s instructions.

Short-term feeding at the wrong time is sufficient to desynchronize peripheral clocks and induce obesity with hyperphagia, physical inactivity and metabolic disorders in mice.

  • https://pubmed.ncbi.nlm.nih.gov/27085778/
  • Yuki Yasumoto, Chiaki Hashimoto, ..., Katsutaka Oishi (2016). Metabolism: clinical and experimental. 2016 Aug 26
  • The Mouse Leptin AssayMax ELISA Kit from Assaypro played a significant role in the study by measuring plasma leptin levels, which were crucial for assessing the impact of feeding sched…

Identification of a Lactobacillus plantarum strain that ameliorates chronic inflammation and metabolic disorders in obese and type 2 diabetic mice

  • https://pubmed.ncbi.nlm.nih.gov/26686731/
  • T. Toshimitsu, J. Mochizuki (2016). Journal of dairy science. 2016 Feb 01
  • .. concentrations were measured using the Adiponectin (Mouse) Total, HMW ELISA Kit (ALPCO, Salem, NH).. Serum leptin concentrations were measured using the AssayMax Mouse Leptin ELISA Kit (Assaypro).. Serum adrenaline and noradrenaline concentrations were measured using the 3-CAT Research ELISA Kit (LDN, ..

Bifidobacterium pseudocatenulatum CECT 7765 Reduces Obesity-Associated Inflammation by Restoring the Lymphocyte-Macrophage Balance and Gut Microbiota Structure in High-Fat Diet-Fed Mice

  • https://pubmed.ncbi.nlm.nih.gov/26161548/
  • Angela Moya-Pérez, Alexander Neef, Yolanda Sanz (2015). PLoS ONE. 2015 Jul 10
  • Hormonal and metabolic parameter analyses Serum leptin concentration was determined by the Assay Max Mouse Leptin ELISA kit (Assay pro, LLC; Ireland) with a sensitivity threshold of 0.3 ng/ml. Biochemical parameters were also quantified in serum using enzymatic kits for glucose (Glucose Liquid Kit), cholesterol (Cholesterol Liquid kit) and triglycerides (Triglyceride Liquid kit, all from Química A

Free Access to a Running-Wheel Advances the Phase of Behavioral and Physiological Circadian Rhythms and Peripheral Molecular Clocks in Mice

  • https://pubmed.ncbi.nlm.nih.gov/25884836/
  • Yuki Yasumoto, Reiko Nakao, ..., Henrik Oster (2015). PLoS ONE. 2015 Jan 23
  • Plasma concentrations of corticosterone, leptin and ghrelin were measured using AssayMax Corticosterone ELISA kits (AssayPro LLC, St. Charles, Missouri, USA), Mouse Leptin ELISA kits (Morinaga Institute of Biological Science Inc., Kanagawa, Japan) and Desacyl-Ghrelin ELISA kits (Mitsubishi Chemical Medience Corporation, Tokyo, Japan), respectively.

Decrease of Obesity by Allantoin via Imidazoline I 1 -Receptor Activation in High Fat Diet-Fed Mice.

  • https://pubmed.ncbi.nlm.nih.gov/23606885/
  • Hsien-Hui Chung, Kung Shing Lee, Juei-Tang Cheng (2013). Evidence-based Complementary and Alternati…. 2013 Apr 23
  • .. the same time point. the leptin concentration was determined using enzymelinked immunosorbent assay (ELISA).. According to the assay procedure, the determination of leptin in samples was carried out using a commercially available mouse ELISA kit (Assaypro, St. Charles, MO, USA).. The absorbance was measured by a SpectraMax 340PC ELISA reader (Molecular Devices Corporation, Union ..

Effect of maternal obesity on estrous cyclicity, embryo development and blastocyst gene expression in a mouse model

  • https://pubmed.ncbi.nlm.nih.gov/23001779/
  • Pablo Bermejo-Alvarez, Cheryl S Rosenfeld, R Michael Roberts (2012). Human reproduction (Oxford, England). 2012 Sep 20
  • .. Tarrytown, NY, USA; detection range 70–333 mg/dl, inter-assay variation 2.3%).. Levels of individual hormones in serum were measured in duplicates on a single day with commercially available mouse ELISA kits by following the manufacturers' instructions (Insulin, Mercodia 10-1247-01, Uppsala, Sweden; Adiponectin Assaypro EMA2500-1, St Charles, MO, USA; Leptin, Assaypro EML2001-1, St Charles, MO, USA).. The detection ranges were 0.2–6.5 µg/ml, 0.7–25 µg/ml and 0.4–30 ..

Bezafibrate regulates the expression and enzyme activity of 11beta-hydroxysteroid dehydrogenase type 1 in murine adipose tissue and 3T3-L1 adipocytes.

  • https://pubmed.ncbi.nlm.nih.gov/17190905/
  • Shigeru Nakano, Yoichi Inada, ..., Kazuwa Nakao (2007). American Journal of Physiology-Endocrinolo…. 2007 May 21
  • In the study, the Mouse Leptin AssayMax ELISA Kit from Assaypro was employed to measure plasma leptin concentrations, which played a significant role in evaluating the metabolic parame…

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