Rat Brain Natriuretic Peptide 45 (BNP-45) ELISA Kit

$407.00

Catalog Number: ERB1202-1

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
Product Size:96 Well Plate

Additional information

Format

Sandwich ELISA

Entrez Gene

25105

Components

Biotinylated Rat BNP-45 Antibody, Chromogen Substrate (1x), MIX Diluent Concentrate (10x), Rat BNP-45 Microplate, Rat BNP-45 Standard, Sealing Tapes, SP Conjugate (100x), Stop Solution (1x), Wash Buffer Concentrate (20x)

TIme

4 hours

Sensitivity

8.7 pg/ml

Range

15.625 – 1000 pg/ml

Samples Type

Plasma, Serum

Associated Disease

Cardiovascular

Species

Rat

UniProt

P13205

Storage

Refer to component labels for details

Plex

1

Usage

For Research Use Only, Not To Be Used For Diagnostic Purposes

Documentation

Publications citing Assaypro: Rat Brain Natriuretic Peptide 45 (BNP-45) ELISA Kit... (38 results)

Please use discretion when following external links; Assaypro cannot guarantee the validity and security of all citation links provided.

Inhalation of printer-emitted particles impairs cardiac conduction, hemodynamics, and autonomic regulation and induces arrhythmia and electrical remodeling in rats

  • https://link.springer.com/article/10.1186/s12989-019-0335-z
  • AP Carll, R Salatini, SV Pirela, Y Wang, Z Xie - Particle and Fibre , 2020 - Springer
  • Background Using engineered nanomaterial-based toners, laser printers generate aerosols with alarming levels of nanoparticles that bear high bioactivity and potential health risks. Yet

Dialogues entre les voies de signalisation de l'AMPc et celles de l'IL-1 dans la rgulation de l'expression de l'IL-6 et rle de l'IL-6 dans les thyrocytes et dans les

  • https://theses.hal.science/tel-00451370/
  • NFL Szabo-Fresnais - 2009 - theses.hal.science
  • L'interleukine-6 (IL-6) est une cytokine associe aux maladies auto-immunes de la glande thyrode et qui pourrait aussi avoir un rle dans le dveloppement de l'hypertrophie

UNIVERSITE PARIS XI FACULTE DE MEDECINE PARIS-SUD

ProT-alpha gene transfer attenuates cardiopulmonary remedying and mortality in a flow-induced pulmonary hypertension rat model

Comparison of Human Adipose-Derived Stem cells and Bone Marrow-Derived Stem cells in a Myocardial Infarction Model

Heart dysfunction induced by choline-deficiency in adult rats: the protective role of L-carnitine

Boerhaavia diffusa L. attenuates angiotensin II-induced hypertrophy in H9c2 cardiac myoblast cells via modulating oxidative stress and down-regulating NF-?Beta and transforming growth factor beta1

Bone marrow mononuclear cells induce beneficial remodeling and reduce diastolic dysfunction in the left ventricle of hypertensive SS/MCWi rats

Phosphorylation of Akt/GSK-3Beta/eNOS amplifies 5-HT2B receptor blockade mediated anti-hypertrophic effect in rats

Telmisartan, a unique ARB, improves left ventricular remodeling of infarcted heart by activating PPAR gamma. Laboratory investigation a journal of technical methods and pathology

Enhanced myocardial vitamin C accumulation in left ventricular hypertrophy in rats is not attenuated with transition to heart failure

Chromogranin B Regulates Calcium Signaling, Nuclear Factor kappaB Activity, and Brain Natriuretic Peptide Production in Cardiomyocytes

Inhibition of NF-kappaB improves left ventricular remodeling and cardiac dysfunction after myocardial infarction

Anti-inflammatory effects of endothelin receptor blockade in left atrial tissue of spontaneously hypertensive rats

  • https://pubmed.ncbi.nlm.nih.gov/35879971/
  • A. Bukowska, Y. Nikonova (2022). International Journal of Cardiology. Heart…. 2022 Jul 20
  • .. filter 505 nm long pass and emission wavelength between filter of 535/50 nm. ... 2.3 Determination of plasma BNP, ANP and atrial levels of 8-isoprostanes, AngII and ET-1Plasma concentrations of rat BNP-45 (Assay Pro) and rat ANP (BioCat) were measured according to the manufactures’s protocol.. Absorbance was recorded at 450 nm, and the concentrations were calculated from a standard ..

AMPK Activation Is Indispensable for the Protective Effects of Caloric Restriction on Left Ventricular Function in Postinfarct Myocardium

  • https://pubmed.ncbi.nlm.nih.gov/35336822/
  • Bernd Niemann, Ruping Pan (2022). Biology. 2022 Mar 16
  • Plasma BNP, adiponectin, leptin and insulin concentrations were measured by using commercial enzyme-linked immunosorbent assays (rat BNP ELISA kit, Assay Pro, #ERB1202-1; mouse BNP ELISA kit, RayBiotech, Peachtree Corners, GA, USA, #EIAM-BNP-1; mouse/rat HMW adiponectin ELISA kit, BioVendor,#638-13079; mouse/rat leptin ELISA kit, BioVendor, Brno, Czech Republic, #RD291001200R; rat/mouse insulin ELISA kit, LINCO Research Inc., Saint Charles, MI, USA, #EZRMI-13K).

Caloric restriction reduces sympathetic activity similar to beta-blockers but conveys additional mitochondrio-protective effects in aged myocardium

  • https://pubmed.ncbi.nlm.nih.gov/33479375/
  • Bernd Niemann, Ling Li, ..., Susanne Rohrbach (2021). Scientific Reports. 2021 Jan 21
  • Plasma analysesPlasma BNP, adiponectin and leptin concentrations were measured by Enzyme-Linked-Immunosorbent-Assays (Rat BNP ELISA-kit, Assay Pro; Mouse/Rat Adiponectin ELISA-kit, B-Bridge International Inc.; Mouse/Rat Leptin ELISA-kit, BioVendor). Plasma insulin concentration was determined by radioimmunoassay (LINCO Research Inc.). Glucose concentrations were measured with the Glucose assay kit

ProT-α gene transfer attenuates cardiopulmonary remedying and mortality in a flow-induced pulmonary hypertension rat model

  • https://pubmed.ncbi.nlm.nih.gov/32593559/
  • Jun-Neng Roan, Chih-Hsin Hsu, ..., Chen-Fuh Lam (2020). The Journal of Heart and Lung Transplantat…. 2020 Oct 01
  • Abstract: ProT is a cell survival gene, which modulates oxidative stress and transforming growth factor (TGF)-β signaling. We hypothesized that the delivery of the ProT cDNA gene in rats could protect…

Inhalation of printer-emitted particles impairs cardiac conduction, hemodynamics, and autonomic regulation and induces arrhythmia and electrical remodeling in rats

  • https://pubmed.ncbi.nlm.nih.gov/31996220/
  • Alex P. Carll, Renata Salatini, PhilipPhilip Demokritou Demokritou (2020). Particle and Fibre Toxicology. 2020 Jan 29
  • .. (Roche, NJ) with Infinity Creatinine Reagent (Thermo Fisher Scientific, MA).. Blood was assayed for B-type natriuretic peptide (BNP-45, AssayPro ERB1202–1), n-terminal propeptide of atrial natriuretic peptide (NT-proANP, Biomedica BI-20892), and cardiac troponin I (Life Diagnostics, CTNI-2-US Ultra-Sensitive Rat Cardiac Troponin-I) according to manufacturer-specified protocols.. In a separate study, hearts from rats with chronic LVP ..

Early Detection of Localized Immunity in Experimental Autoimmune Myocarditis Using [99mTc]Fucoidan SPECT

  • https://pubmed.ncbi.nlm.nih.gov/31432389/
  • Jonathan Vigne, Thomas Cognet, ..., François Rouzet (2019). Molecular imaging and biology. 2019 Aug 20
  • Abstract: The aim of the study was to evaluate the ability of technetium-99m-fucoidan ([ 99m Tc]fucoidan), a molecular imaging agent specific for selectins, in the assessment of early localized immuni…

Neuregulin-1β Partially Improves Cardiac Function in Volume-Overload Heart Failure Through Regulation of Abnormal Calcium Handling

  • https://pubmed.ncbi.nlm.nih.gov/31281251/
  • Xuehui Wang, Xiaozhen Zhuo, Aiqun Ma (2019). Frontiers in Pharmacology. 2019 Jun 21
  • Chemicals and antibodies used were as follows: NRG-1β from ProSpec-Tany TechnoGene (Nes Ziona, Israel), rat brain natriuretic peptide (BNP-45) enzyme-linked immunosorbent assay (ELISA) kit from Assaypro (MO, USA), enhanced chemiluminescence (ECL) from Pierce (IL, USA), type II collagenase from Worthington (NJ, USA), Cav1.2 antibody from Alomone (Jerusalem, Israel), NCX1 antibody from Millipore (MA, USA), SERCA2 antibody, β-actin antibody, and horseradish peroxidase (HRP)-labeled secondary antibody from Santa Cruz Biotechnology (CA, USA).

A peptide vaccine targeting angiotensin II attenuates the cardiac dysfunction induced by myocardial infarction

  • https://pubmed.ncbi.nlm.nih.gov/28266578/
  • Ryo Watanabe, Jun-ichi Suzuki, Mitsuaki Isobe (2017). Scientific Reports. 2017 Mar 07
  • The concentrations of cystatin C (“Mouse/Rat Cystatin C Quantikine ELISA Kit”, R & D Systems, Minneapolis, MN, USA), BNP-45 (“Rat BNP-45 ELISA Kit”, Assaypro, St. Charles, MO, USA), and albumin (“Rat Albumin ELISA Quantitation Set”, R & D Systems) were determined using an enzyme-linked immunosorbent assay according to the manufacturer’s instructions.

Aggravated Cardiac Remodeling post Aortocaval Fistula in Unilateral Nephrectomized Rats

  • https://pubmed.ncbi.nlm.nih.gov/26252578/
  • Jie Wu, Zhong Cheng, ..., Shao Hu (2015). PLoS ONE. 2015 Aug 07
  • , America). Plasma creatinine was measured with the Jaffé method with deproteinization. Plasma rBNP-45 concentrations were measured by using a sensitive enzyme-linked immunosorbent assay (ELISA) kit (Assaypro, America). Plasma cystatin C concentrations were measured by using a sensitive enzyme-linked immunosorbent assay (ELISA) kit (R & D Systems, America). An enzymatic method was used for the dete

Myocardial infarction worsens glomerular injury and microalbuminuria in rats with pre-existing renal impairment accompanied by the activation of ER stress and inflammation

  • https://pubmed.ncbi.nlm.nih.gov/25173645/
  • Zhifeng Dong, Penglong Wu (2014). Molecular Biology Reports. 2014 Aug 31
  • Abstract: Deterioration of renal function occurs after chronic heart failure in approximately one-third of patients, particularly in those with pre-existing renal impairment such as diabetic nephropat…

Comparison of Human Adipose-Derived Stem cells and Bone Marrow-Derived Stem cells in a Myocardial Infarction Model

  • https://pubmed.ncbi.nlm.nih.gov/23211469/
  • Jeppe Grøndahl Rasmussen, Ole Frøbert, ..., Ulf Simonsen (2014). Cell Transplantation. 2014 Mar 01
  • In the study, the Rat Brain Natriuretic Peptide 45 BNP 45 AssayMax ELISA Kit from Assaypro was utilized to analyze serum samples for rat BNP-45 levels post-sacrifice. This analysis pla…

MicroRNA 210 as a biomarker for congestive heart failure.

  • https://pubmed.ncbi.nlm.nih.gov/23302636/
  • Kosuke Endo, Yukiko Naito, ..., Naoharu Iwai (2013). Biological & pharmaceutical bulletin. 2013 Jun 26
  • .. Japan).The expression levels were quantified by densitometry (Luminescent Image Analyzer lAS-1000: FUJIFIlM, Tokyo, Japan).. Plasma BNP ELISA Plasma BNP concentrations were assayed using the AssayMax Rat BNP-45 ElISA Kit (AssayPro, MO, U.S.A.) according to the manufacturer’s protocol.. Absorbance at 450 nm was measured using a Wallac 1420 ARVO MX/light system (PerkinElmer, ..

Heart dysfunction induced by choline-deficiency in adult rats: The protective role of l-carnitine

  • https://pubmed.ncbi.nlm.nih.gov/23562624/
  • Athina A. Strilakou, Andreas C. Lazaris (2013). European journal of pharmacology. 2013 Jun 01
  • Abstract: Choline is a B vitamin co-factor and its deficiency seems to impair heart function. Carnitine, a chemical analog of choline, has been used as adjunct in the management of cardiac diseases. T…

Boerhaaviadiffusa L. attenuates angiotensin II-induced hypertrophy in H9c2 cardiac myoblast cells via modulating oxidative stress and down-regulating NF-κβ and transforming growth factor β1

  • https://pubmed.ncbi.nlm.nih.gov/23591029/
  • A Prathapan, V P Vineetha, ..., K G Raghu (2013). British Journal of Nutrition. 2013 Apr 16
  • the cell number(27). Determination of atrial natriuretic peptide and B-type natriuretic peptide Atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) were measured using an ELISA kit (AssayPro). Assay of endogenous antioxidant status and lipid peroxidation Activities of various endogenous antioxidant enzymes were evaluated in the control and treated cells. Catalase activity was assa

Does pretreatment of bone marrow mesenchymal stem cells with 5-azacytidine or double intravenous infusion improve their therapeutic potential for dilated cardiomyopathy?

  • https://pubmed.ncbi.nlm.nih.gov/23314418/
  • Sirui Yang, Jinhua Piao, ..., Yan Zhou (2013). Medical Science Monitor Basic Research. 2013 Jan 01
  • pidly cleaved and have a half-life of 3–4 min, we determined the serum level of N-terminal BNP using an electrochemiluminescence immunoassay (ProBNP Elecsys; Roche Diagnostics GmbH, Germany). The N-terminal BNP levels were measured with an ELISA kit (Assaypro, St. Charles, MO, USA); reproducibility and precision of this assay is well below 5%, even when using high concentrations of N-terminal BNP.

Bone marrow mononuclear cells induce beneficial remodeling and reduce diastolic dysfunction in the left ventricle of hypertensive SS/MCWi rats

  • https://pubmed.ncbi.nlm.nih.gov/22851760/
  • Sarah J Parker, Daniela N Didier, ..., Andrew S Greene (2012). Physiological Genomics. 2012 Oct 01
  • Abstract: Bone marrow mononuclear cells (BMMNCs) increase capillary density and reduce fibrosis in rodents after myocardial infarction, resulting in an overall improvement in left ventricular function…

Renal Sympathetic Denervation Suppresses De Novo Podocyte Injury and Albuminuria in Rats With Aortic Regurgitation

  • https://pubmed.ncbi.nlm.nih.gov/22328542/
  • Kazi Rafiq, Takahisa Noma, ..., Akira Nishiyama (2012). Circulation. 2012 Mar 20
  • Plasma and kidney AngII concentrations were measured by a radioimmunoassay as previously described.24,25 The degree of lipid peroxidation in plasma and renal cortical tissue was evaluated by the use of biochemical assays for thiobarbituric acid reaction substances (TBARS) as previously described.22 Collagen content in the LV tissues was determined from hydroxyproline concentrations as previously described.32 Plasma brain natriuretic peptide (BNP) was measured with an AssayMaz Rat BNP-45 (rBNP-45) assay kit (ASSAYPRO, St. Charles, MO).

Phosphorylation of Akt/GSK-3β/eNOS amplifies 5-HT2B receptor blockade mediated anti-hypertrophic effect in rats

  • https://pubmed.ncbi.nlm.nih.gov/22210189/
  • Saurabh Bharti, Ratnakar Singh, ..., Dharamvir Singh Arya (2011). FEBS letters. 2011 Dec 26
  • Abstract: Herein, we studied the cross talk between 5-HT(2B) receptor blocker (SB-204741) and GSK-3β inhibitor (SB-216763) in isoproterenol-induced cardiac hypertrophy for 28 days. SB-204741 treatment…

Survival, Exercise Capacity, and Left Ventricular Remodeling in a Rat Model of Chronic Mitral Regurgitation: Serial Echocardiography and Pressure-Volume Analysis

  • https://pubmed.ncbi.nlm.nih.gov/22125560/
  • Kyung-Hee Kim, Yong-Jin Kim, Young-Bae Park (2011). Korean Circulation Journal. 2011 Oct 31
  • .. the plasma was stored at -80℃ and assayed for plasma brain natriuretic peptide (BNP).. BNP concentration was assessed with a rat ELISA rat BNP-45 kit (Assaypro, St. Charles, MO, USA).. The unpaired two-sided Student's t-test was used to compare continuous variables between groups, as ..

Preventing progression of cardiac hypertrophy and development of heart failure by paricalcitol therapy in rats

  • https://pubmed.ncbi.nlm.nih.gov/21565836/
  • Soochan Bae, Bhargavi Yalamarti, ..., Peter M Kang (2011). Cardiovascular Research. 2011 Sep 01
  • Tail-vein blood was collected and plasma levels of BNP were measured by using BNP ELISA kit (Assaypro, St. Charles, MO, USA) according to the manufacturer's instructions. 8

A 3-D Cardiac Muscle Construct for Exploring Adult Marrow Stem Cell Based Myocardial Regeneration

  • https://pubmed.ncbi.nlm.nih.gov/20129663/
  • Mani T. Valarmathi, Richard L. Goodwin, ..., Jay D. Potts (2011). pmc02887929. 2011 Apr 01
  • .. cell culture media was centrifuged at 2,000g for 10 minutes to remove any debris.. Aliquots of 50 μl supernatant (1:4 dilution) were analyzed for ANP using AssayMax Rat ANP ELISA Kit (ERA 7010-1, Assaypro), and for BNP using AssayMax Rat BNP-45 (rBNP-45) ELISA Kit (ERB 1202-1, Assaypro) according to manufacturer's instructions, respectively.. The absorbance was read at 450 nm immediately after the addition of reagents.The absorbance ..

Telmisartan, a unique ARB, improves left ventricular remodeling of infarcted heart by activating PPAR gamma

  • https://pubmed.ncbi.nlm.nih.gov/21403641/
  • Yasuhiro Maejima, Hiroyuki Okada, ..., Mitsuaki Isobe (2011). Laboratory Investigation. 2011 Mar 14
  • Abstract: Unfavorable left ventricular (LV) remodeling after myocardial infarction (MI) leads to cardiac dysfunction. We examined whether Telmisartan, an angiotensin (Ang) II type I receptor blocker (…

Direct epicardial shock wave therapy improves ventricular function and induces angiogenesis in ischemic heart failure.

  • https://pubmed.ncbi.nlm.nih.gov/19327525/
  • Daniel Zimpfer, Seyedhossein Aharinejad, ..., Michael Grimm (2009). The Journal of Thoracic and Cardiovascular…. 2009 Apr 15
  • Enzyme-linked immunosorbent assay for rat BNP-45 (AssayMax; AssayPro, St Charles, Mo) was performed according to the manufacturer’s protocol.. Fifty microliters of standard or a serum sample were added to each well.Fifty microliters ..

A novel IKK inhibitor suppresses heart failure and chronic remodeling after myocardial ischemia via MMP alteration

  • https://pubmed.ncbi.nlm.nih.gov/19007316/
  • Shin Wakatsuki, Jun-ichi Suzuki, ..., Mitsuaki Isobe (2008). Expert Opinion on Therapeutic Targets. 2008 Nov 13
  • Abstract: Amplification of inflammatory response in the non-infarct area plays an important role in the pathogenesis of ventricular remodeling after myocardial ischemia. Activation of nuclear factor-k…

Activated vitamin D attenuates left ventricular abnormalities induced by dietary sodium in Dahl salt-sensitive animals

  • https://pubmed.ncbi.nlm.nih.gov/17942703/
  • Natalya Bodyak, Juan Carlos Ayus, ..., Peter M Kang (2007). Proceedings of the National Academy of Sci…. 2007 Oct 23
  • Tail-vein blood was collected at baseline, 3, and 6 weeks (at sacrifice) after initiation of a HS diet. Plasma levels of BNP were measured by using BNP ELISA kit (Assaypro, St. Charles, MO) according to the manufacturer's instructions.

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