[Ser2]-Neuromedin C (GRP) Antibody (Biotin Conjugate)

Sexual Experience Induces the Expression of Gastrin-Releasing Peptide and Oxytocin Receptors in the Spinal Ejaculation Generator in Rats

• https://pubmed.ncbi.nlm.nih.gov/34638701/
• Takumi Oti, Ryota Ueda, ..., Hirotaka Sakamoto (2021). International Journal of Molecular Science…. 2021 Sep 26
• ...goat serum and 1% BSA in PBS containing 0.3% Triton X-100 for 30 min at room temperature, the sections were then incubated with the primary rabbit antiserum against GRP (1:2000 dilution) (11081; AssayPro, St. Charles, MO, USA, Research Resource Identifier (RRID): AB_2571636) as described elsewhere [ 14 , 29 , 30 , 31 ]. In this study, we performed immunostaining for GRP as the marker for d

The gastrin-releasing peptide/bombesin system revisited by a reverse-evolutionary study considering Xenopus

• https://pubmed.ncbi.nlm.nih.gov/34172791/
• Asuka Hirooka, Mayuko Hamada, ..., Hirotaka Sakamoto (2021). Scientific Reports. 2021 Jun 25
• at room temperature, sections were then incubated in Can Get Signal A (TOYOBO) with a 1:2000 dilution of primary rabbit antiserum against rat GRP20–29 , a 10-amino acid-peptide called NMC or GRP-10 (AssayPro, St. Charles, MO, RRID: AB_2571636) for 48 h at 4 °C. This GRP antiserum has previously been shown to be specific for rodent GRP 20, 46 . The amino acid sequence of NMC (epitope residues) is i

Oxytocin Influences Male Sexual Activity via Non-synaptic Axonal Release in the Spinal Cord

• https://pubmed.ncbi.nlm.nih.gov/33125871/
• Takumi Oti, Keita Satoh, ..., Hirotaka Sakamoto (2020). Current biology : CB. 2020 Oct 29
• Abstract: Oxytocinergic neurons in the paraventricular nucleus of the hypothalamus that project to extrahypothalamic brain areas and the lumbar spinal cord play an important role in the control of ere…

Effects of Sex Steroids on the Spinal Gastrin-Releasing Peptide System Controlling Male Sexual Function in Rats.

• https://pubmed.ncbi.nlm.nih.gov/29534195/
• Takumi Oti, Keiko Takanami, ..., Hirotaka Sakamoto (2018). Endocrinology. 2018 Nov 27
• ... Species Raised in; Monoclonal or Polyclonal Dilution Used RRID Anti-GFP Cell Signaling Technology, 2956S Rabbit; monoclonal 1:1000 AB_1196615 Anti-GFP RockLand, 600-901-215 Chicken; polyclonal 1:5000 AB_1537402 Anti–neuromedin C (GRP) AssayPro, 11081-05015 Rabbit; polyclonal 1:1000 AB_2571636 Anti-PR Abcam, ab27596 Rabbit; monoclonal 1:200 AB_471063 Downloaded from https://academic.oup.com/endo/article-abstract/159/4/1886/4924486 by Oregon Health & Science University user on 14 May ..

Identification of the sexually dimorphic gastrin-releasing peptide system in the lumbosacral spinal cord that controls male reproductive function in the mouse and Asian house musk shrew (Suncus murinus)

• https://pubmed.ncbi.nlm.nih.gov/27804131/
• Kei Tamura, Yasuhisa Kobayashi, ..., Hirotaka Sakamoto (2017). Journal of Comparative Neurology. 2017 Feb 27
• Triton X-100 for 1hr at room temperature, sections were incubated with primary rabbit antiserum against mouse GRP20-29 (1:2,000 dilution), a 10-amino acid-peptide called neuromedin C (NMC) or GRP-10 (AssayPro, St. Charles, MO) for 48hr at 4 8C. The GRP antiserum used in this study has previously been shown to be specific for rodent GRP in the spinal cord (Satoh et al., 2015b,c; Takanami et al., 201