Human Leptin ELISA Kit

$407.00

Catalog Number: EL2001-1

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
Product Size:96 Well Plate

Catalog Number. EL2001-1 Categories: , , Tag: Brand:

Additional information

Format

Sandwich ELISA

Entrez Gene

3952

Components

Biotinylated Human Leptin Antibody, Chromogen Substrate (1x), Human Leptin Microplate, Human Leptin Standard, MIX Diluent Concentrate (10x), Sealing Tapes, SP Conjugate (100x), Standard Diluent (1x), Stop Solution (1x), Wash Buffer Concentrate (20x)

TIme

4 hours

Sensitivity

45 pg/ml

Range

0.125 – 32 ng/ml; 0.234 – 60 IU/ml

Samples Type

Cell Culture Supernatants, Plasma, Serum

Associated Disease

Diabetes

Species

Human

UniProt

P41159

Storage

Refer to component labels for details

Plex

1

Usage

For Research Use Only, Not To Be Used For Diagnostic Purposes

Documentation

Publications (80 results)

Please use discretion when following external links; Assaypro cannot guarantee the validity and security of all citation links provided.

Leptin levels in vitreous fluids of patients with diabetic retinopathy

  • https://jrmi.pk/article/view/5
  • AAS Bukhari, N Parveen, Q Khan, K Khan - Journal of Rehman Medical …, 2015 - jrmi.pk
  • … The enzyme immunoassay test followed a typical two-step capture or “sandwich” type assay by using AssayMax Human Leptin EliSA Kit (Assaypro, Belgium). Data were analyzed using …

Serum leptin, adiponectin, resistin and ghrelin levels in psoriatic patients treated with cyclosporin

Leptin and leptin receptor gene polymorphisms and their association with plasma leptin levels and obesity in a multi-ethnic Malaysian suburban population

  • https://link.springer.com/article/10.1186/1880-6805-33-15
  • SH Fan, YH Say - Journal of physiological anthropology, 2014 - Springer
  • Background This study was to investigate the prevalence of single nucleotide polymorphisms (SNPs) in leptin gene LEP (A19G and G2548A) and leptin receptor gene LEPR (K109R …

Esat Imal A, Cagil E, Mengi M, Ekmekci H, Sahmay S, Nalbantoglu B, Donma MM

  • https://www.academia.edu/download/103743419/107320150302.pdf
  • F Yildizfer - vitro fertilization, levels of pro-inflammatory factors and …, 2015 - academia.edu
  • … CV were 4.0 and 7.2%, respectively, using the AssayMax Human Leptin ELISA kit (Assaypro, USA). The intra-assay and inter-assay CV were 4.0 and 7.7%, respectively, using the …

Association of vitamin D supplementation with serum leptin and metabolic parameters in Egyptian patients with non-alcoholic steatohepatitis: a prospective study

Evaluation of body weight, insulin resistance, leptin and adiponectin levels in premenopausal women with hyperprolactinemia

  • https://link.springer.com/article/10.1007/s12020-013-9931-0
  • A Atmaca, B Bilgici, GC Ecemis, OK Tuncel - Endocrine, 2013 - Springer
  • The effects of hyperprolactinemia on metabolic parameters are not clear and a few data evaluating adiponectin levels in prolactinoma and idiopathic hyperprolactinemia exist. The aim …

The role of adenovirus 36 as a risk factor in obesity: the first clinical study made in the fatty tissues of adults in Turkey

  • https://www.sciencedirect.com/science/article/pii/S0882401015000273
  • S Ergin, E Altan, O Pilanci, S Sirekbasan, O Cortuk… - Microbial …, 2015 - Elsevier
  • Obesity which developes due to multifactorial reasons, was associated recently with human Adenovirus-36 (Ad-36). The aim of this study was to investigate the prevalence of Ad-36 …

Association of polymorphism in adiponectin (+ 45 T/G) and leptin (–2548 G/A) genes with type 2 diabetes mellitus in male Egyptians

Leptin as an important link between obesity and cardiovascular risk factors in men with acute myocardial infarction

The effects of 17β-estradiol plus drospirenone on anthropometric and biochemical measures of adiposity in menopausal women

  • https://link.springer.com/article/10.1007/s00404-012-2437-9
  • M Karakus, R Gelisgen, A Topcuoglu, O Guralp… - Archives of gynecology …, 2012 - Springer
  • Purpose To assess whether there are changes on anthropometric and biochemical measures of adiposity in pre- and postmenopausal women and in the latter before and after 6 …

Tomato juice supplementation in young women reduces inflammatory adipokine levels independently of body fat reduction

Circulating and adipose tissue gene expression of zinc-α2-glycoprotein in obesity: its relationship with adipokine and lipolytic gene markers in subcutaneous and …

  • https://academic.oup.com/jcem/article-abstract/94/12/5062/2597285
  • V Ceperuelo-Mallafré, S Naf, X Escoté… - The Journal of …, 2009 - academic.oup.com
  • Context: Zinc-α2-glycoprotein (ZAG) is a soluble protein similar to the class I major histocompatibility complex heavy chain, which has been implicated in lipid catabolism. We …

In vitro fertilization, levels of pro-inflammatory factors and lipid peroxidation

  • https://pmc.ncbi.nlm.nih.gov/articles/PMC4671383/
  • F Yildizfer, O Donma, M Yen, O Ekmekci… - … Journal of Fertility & …, 2015 - pmc.ncbi.nlm.nih.gov
  • … Human Resistin ELISA kit (Assaypro, USA). The intra-assay and inter-assay CV were 4.0 and 7.2%, respectively, using the AssayMax Human Leptin ELISA kit (Assaypro, USA). The intra…

Clinical significance of serum leptin level in patients with gastric cancer

  • https://link.springer.com/article/10.1684/ecn.2018.0408
  • F Tas, S Karabulut, K Erturk, D Duranyildiz - European Cytokine Network, 2018 - Springer
  • Leptin may support the proliferation and hinder the apoptosis of tumor cells. Although leptin expression has been studied in several human tumors, its potential clinical significance …

Ventricular hypertrophy and cavity dilatation in relation to body mass index in women with uncomplicated obesity

  • https://heart.bmj.com/content/97/3/203.short
  • OJ Rider, SE Petersen, JM Francis, MK Ali… - Heart, 2011 - heart.bmj.com
  • Objective The traditionally accepted mechanism for ventricular adaptation to obesity suggests that cavity dilatation in response to increased blood volume and elevated filling pressure …

LEVELS OF SERUM INTERLEUKIN IL-6 IN PATIENTS WITH ADVANCED DIABETIC RETINOPATHY

  • https://jrmi.pk/article/view/3
  • AAS Bukhari, N Parveen, Q Khan, K Khan - Journal of Rehman Medical …, 2015 - jrmi.pk
  • … The enzyme immunoassay test followed a typical two-step capture or “sandwich” type assay by using AssayMax Human Leptin EliSA Kit (Assaypro, Belgium). Data were analyzed using …

Serum leptin level and waist-to-hip ratio (WHR) predict the overall survival of metastatic breast cancer (MBC) patients treated with aromatase inhibitors (AIs)

  • https://link.springer.com/article/10.1007/s12282-011-0322-1
  • M Artac, H Bozcuk, A Kıyıcı, OO Eren, MC Boruban… - Breast Cancer, 2013 - Springer
  • Introduction Our objective was to determine whether serum leptin levels and obesity-related factors could affect outcome for metastatic breast cancer (MBC) patients treated with …

Effect of uvulopalatopharyngoplasty on leptin and endothelial function in sleep apnea

  • https://journals.sagepub.com/doi/abs/10.1177/0003489414521385
  • CC Lin, YP Wang, KS Lee, SF Liaw… - Annals of Otology …, 2014 - journals.sagepub.com
  • … The serum leptin concentration was measured by enzyme-linked immunosorbent assay with the AssayMax Human Leptin ELISA Kit (AssayPro, St Charles, Missouri). The samples were …

Obesity and CRP, adiponectin, leptin, and lipid profile in Saudi Arabian adolescent females

Genetic, dietary, and non-dietary risk factors of obesity among preparatory-year female students at Taibah University, Saudi Arabia

  • https://www.sciencedirect.com/science/article/pii/S1658365516300401
  • DE El Nashar, KM Alananbeh, N Al Hassan - Journal of Taibah University …, 2017 - Elsevier
  • Genetic factors have a strong influence on obesity and are associated with body mass index (BMI). No study has investigated the relationships between glucose, the fat mass and …

Leptin and adiponectin, but not IL18, are related with insulin resistance in treated HIV-1-infected patients with lipodystrophy

Роль адипокинов в прогрессировании колоректального рака

Moringa () leaves aqueous extract enhances fibronectin type III domain-containing protein 5 gene expression and serum irisin liberation in an obesity model

  • https://journals.ekb.eg/article_408609.html
  • RK Abdellah, NM El-Beih, FA Morsy… - Egyptian …, 2021 - journals.ekb.eg
  • … Serum leptin concentration was measured using reagent kits (EL2001-1) purchased from Assaypro (USA). Irisin and tumor necrosis factor α (TNFα) levels were measured using reagent …

A study of fatty acid binding protein 4 in HIV‐1 infection and in combination antiretroviral therapy‐related metabolic disturbances and lipodystrophy

Interrelationship between bone turnover markers, calciotropic hormones and leptin in obese Saudi children.

  • https://www.europeanreview.org/wp/wp-content/uploads/4332-4343.pdf
  • LM Saber, HNF Mahran, HH Baghdadi… - European Review for …, 2015 - europeanreview.org
  • … EL2001-1 that has a range of 0.00-32 ng/mL. The ASSAYPRO Leptin ELISA is an enzyme immunoassay for the measurement of leptin in serum and plasma. By determining this bone …

Association between obstructive sleep apnea severity and endothelial dysfunction in patients with type 2 diabetes

  • https://link.springer.com/article/10.1186/s12933-017-0521-y
  • V Bironneau, F Goupil, PH Ducluzeau… - Cardiovascular …, 2017 - Springer
  • Background Obstructive sleep apnea (OSA) and type 2 diabetes (T2D) are associated with endothelial dysfunction a main predictor of late cardiovascular (CV) events. Despite the high …

Effect of Fasting Therapy and Low-Calorie Diet on Anthropometric and Serum Lipids in Obese Females

Interrelationships between obesity and bone markers in post-menopausal women with either obesity or osteoporosis

  • https://www.sciencedirect.com/science/article/pii/S1878764914001521
  • A Doventas, IM Bolayirli, S Incir, H Yavuzer… - European Geriatric …, 2015 - Elsevier
  • Purpose Obesity and bone metabolism are interrelated. The aim of this study is to investigate the relationship between obesity and bone markers, leptin and insulin resistance in post-…

Maternal leptin, adiponectin, resistin, visfatin and tumor necrosis factor-alpha in normal and gestational diabetes

  • https://link.springer.com/article/10.1007/s12291-013-0394-0
  • AFH Noureldeen, SY Qusti, MN Al-Seeni… - Indian Journal of Clinical …, 2014 - Springer
  • … Hormonal assay: Specific ELISA kits purchased from Assaypro Company were employed for determination of: Serum leptin (catalog no. EL2001-1). The assay sensitivity was ~0.12 ng/…

Correlation of C1q/TNF-related protein-3 with cardiac autonomic tone and metabolic parameters in obesity

  • https://pmc.ncbi.nlm.nih.gov/articles/PMC6628617/
  • NB Jain, A Yadav, N Shankar… - International Journal of …, 2019 - pmc.ncbi.nlm.nih.gov
  • … Human ™ Adiponectin ELISA research kit by Assaypro LLC, designed to detect adiponectin in … Leptin was estimated using commercial human leptin ELISA kit from Boster Biological …

Siklosporin tedavisi alan psoriasisli hastalarda serum leptin, adiponektin, resistin ve ghrelin düzeyleri

  • no link
  • M Yüksel - 2010 - Necmettin Erbakan Üniversitesi …
  • no snippet

Adipokines as New Biomarkers of Immune Recovery: Apelin Receptor, RBP4 and ZAG Are Related to CD4+ T-Cell Reconstitution in PLHIV on Suppressive …

  • https://www.mdpi.com/1422-0067/23/4/2202
  • E Yeregui, J Masip, C Viladés, P Domingo… - International journal of …, 2022 - mdpi.com
  • A significant proportion of people living with HIV (PLHIV) who successfully achieve virological suppression fail to recover CD4 + T-cell counts. Since adipose tissue has been discovered …

Impact of serum adipokines on tumor mitotic and apoptotic activity in endometrial cancer

  • https://dergipark.org.tr/tr/doi/10.28982/josam.581498
  • A Özdemir, P Karlı, A Kökçü, M Kefeli - Journal of Surgery and …, 2019 - dergipark.org.tr
  • Aim: Endometrial cancer is the most common malignancy of female genital system. Obesity is one of the most important risk factors in endometrial cancer. Adipose tissue functions just …

Adipokines, psoriasis, systemic inflammation, and endothelial dysfunction

  • https://onlinelibrary.wiley.com/doi/abs/10.1111/ijd.13699
  • M Lynch, T Ahern, CM Sweeney… - International journal …, 2017 - Wiley Online Library
  • Adipokines are secreted by white adipose tissue, an active endocrine organ, and play a role in the regulation of metabolic functions such as lipid metabolism, inflammation, and …

Efecto de la terapia UVB de banda estrecha sobre la síntesis de vitamina D y los parámetros de síndrome metabólico en pacientes con psoriasis moderada y grave

  • https://ddd.uab.cat/record/113015
  • J Romaní de Gabriel - 2013 - ddd.uab.cat
  • La psoriasis es una enfermedad cutánea crónica que ha sido relacionada con el síndrome metabólico. El objetivo de este trabajo fue evaluar en pacientes con psoriasis la presencia …

La implicación de las quimiocinas y las adipoquinas en la respuesta inmune discordante en la infección por el VIH

  • https://www.tdx.cat/handle/10803/674997
  • E Yeregui Etxebarria - tdx.cat
  • Desde el inicio del tratamiento antiretroviral, la mortalidad y morbilidad por VIH han disminuido drásticamente, pero aún así, un porcentaje no desdeñable de personas que viven con …

* Abdellah RK et al. (2021) Moringa (Moringa oleifera) leaves aqueous extract enhances fibronectin type III domain-containing protein 5 gene expression and serum irisin liberation in an obesity model. Egyp. Pharma. J. 20(1):92-103.

* Ozgokce C et al. (2020) C?Reactive Protein, Fibrinogen, Leptin, and Adiponectin Levels in Women with Polycystic Ovary Syndrome. J Obstet Gynaecol India. 70(6):490-496.

* Kaisar HH et al. (2020) Association of vitamin D supplementation with serum leptin and metabolic parameters in Egyptian patients with non-alcoholic steatohepatitis: a prospective study. Egyptian Liver J. 38 (2020).

* Honnegowda TM et al. (2020) Effect of Fasting Therapy and Low-Calorie Diet on Anthropometric and Serum Lipids in Obese Females. J. Diabetes and Endocrine Practice. 3(2).

* Cheng CF et al. (2018) Monascus-fermented red mold dioscorea protects mice against alcohol-induced liver injury, whereas its metabolites ankaflavin and monascin regulate ethanol-induced peroxisome proliferator-activated receptor-gamma and sterol regulatory element-binding transcription factor-1 expression in HepG2 cells.J Sci Food Agric. 98(5):1889-1898.

* Qureshi IZ et al. (2017) Serum APOE, leptin, CFH and HTRA1 levels in Pakistani age related macular degeneration patients. J Pak Med Assoc. 67(6):852-857.

* Lynch M et al. (2017) Adipokines, psoriasis, systemic inflammation, and endothelial dysfunction. Int J Dermatol. 56(11):1103-1118.

* Kahraman R et al. (2017) Are adipocytokines inflammatory or metabolic mediators in patients with inflammatory bowel disease? Therapeutics and Clinical Risk Management. 13:1295-1301

* Ekmen N (2016) Leptin as an important link between obesity and cardiovascular risk factors in men with acute myocardial infarction. Indian Heart J. 2016 Mar-Apr;68(2):132-7

* Bilir BE et al. (2016) The effects of fat distribution and some adipokines on insulin resistance in subjects with prediabetes. Endokrynologia Polska 67 (3):277-282

* Ahmed S and Jahan S (2016) Possible Role of Neuromedin S (NMS) in Male Reproduction: Effect of NMS on Adipokines Secretion in Male Rhesus Monkeys (Macaca mulatta). Pakistan J. Zool., 49 (1):273-279

* Acquah S et al. (2016) Elevated adiponectin but varied response in circulating leptin levels to falciparum malaria in type 2 diabetics and non-diabetic controls. Biomed J. 39(5):346-353.

* Yildizfer F et al. (2015) In Vitro Fertilization, Levels of Pro-Inflammatory Factors and Lipid Peroxidation

* Wabitsch M et al. (2015) Severe Early-Onset Obesity Due to Bioinactive Leptin Caused by a p.N103K Mutation in the Leptin Gene. J Clin Endocrinol Metab. 100(9):3227-30

* Shafik NM (2015) Impact of Nigella Sativa, Omega-3 Fatty Acids and Chromium Picolinate on NF-kappaB /leptin-insulin Axis in Obese Subjects with Non-alcoholic Fatty Liver Disease. American Journal of Medical and Biological Research. 3 (6):139-145

* Saber LM et al. (1015) Interrelationship between bone turnover markers, calciotropic hormones and leptin in obese Saudi children. Eur Rev Med Pharmacol Sci. 19(22):4332-43

* Ergin S et al. (2015) The role of adenovirus 36 as a risk factor in obesity: The first clinical study made in the fatty tissues of adults in Turkey. Microbial Pathogenesis 80(2015):57-62

* Wahab F et al. (2014) Primate HPT axis response to the peripheral kisspeptin challenge under different time periods of food restriction in monkeys. Horm Metab Res 2014; 46(03): 187-192.

* Lin CC et al. (2014) Effect of Uvulopalatopharyngoplasty on Leptin and Endothelial Function in Sleep Apnea. Ann Otol Rhinol Laryngol. 2014 Jan;123(1):40-6. doi: 10.1177/0003489414521385

* Fan SH and Say YH (2014) Leptin and leptin receptor gene polymorphisms and their association with plasma leptin levels and obesity in a multi-ethnic Malaysian suburban population.J Physiol Anthropol. 33:15. doi: 10.1186/1880-6805-33-15

* Romani J et al. (2013) Circulating levels of lipocalin-2 and retinol-binding protein-4 are increased in psoriatic patients and correlated with baseline PASI. Arch Dermatol Res. 305(2):105-12.

* Ojeda I et al. (2013) A disposable electrochemical immunosensor for the determination of leptin in serum and breast milk. Analyst. 138(15):4284-91.

* Mahassni SH and Sebaa RB (2013) Obesity and CRP, Adiponectin, Leptin, and Lipid Profile in Saudi Arabian Adolescent Females. Journal of Basic & Applied Sciences. 9:500-509

* Atmaca A et al. (2013) Evaluation of body weight, insulin resistance, leptin and adiponectin levels in premenopausal women with hyperprolactinemia. Endocrine. 44(3):756-61.

* Artac M et al. (2013) Serum leptin level and waist-to-hip ratio (WHR) predict the overall survival of metastatic breast cancer (MBC) patients treated with aromatase inhibitors (AIs). Breast Cancer. 20(2):174-80. doi: 10.1007/s12282-011-0322-1. Epub 2011 Dec 28

* Schroyen B (2012) Leptin-mediated reactive oxygen species production does not significantly affect primary mouse hepatocyte functions in vitro. Eur J Gastroenterol Hepatol. 24(12):1370-80.

* Rosenzweig T (2012) PHARMACEUTICAL COMPOSITIONS COMPRISING EXTRACTS OF Sarcopoterium spinosum, COMPONENTS THEREOF, AND USES THEREOF. Patent Application: US20120076877 A1

* Romani J (2012) Effect of narrow-band UVB therapy on inflammatory markers and body fat composition in moderate to severe psoriasis. Br J Dermatol. 166(6):1237-44.

* Ceperuelo-Mallafre V et al. (2012) Zinc alpha-2 glycoprotein is implicated in dyslipidaemia in HIV-1-infected patients treated with antiretroviral drugs. HIV Med. 13(5):297-303.

* Yildizhan R et al. (2011) Serum retinol-binding protein 4, leptin, and plasma asymmetric dimethylarginine levels in obese and nonobese young women with polycystic ovary syndrome. Fertil Steril. 96(1):246-50. Epub 2011 May 19

* Wahab F et al. (2011) Decrease in Hypothalamic Kiss1 and Kiss1r Expression: A Potential Mechanism for Fasting-induced Suppression of the HPG Axis in the Adult Male Rhesus Monkey (Macaca mulatta). Horm Metab Res. 43(2):81-85

* Rider OJ et al. (2011) Ventricular hypertrophy and cavity dilatation in relation to body mass index in females with uncomplicated obesity. Heart. 97(3):203-208

* Osorio-Conles O et al. (2011) Plasma PTX3 protein levels inversely correlate with insulin secretion and obesity, while visceral adipose tissue PTX3 gene expression is increased in obesity. Am J Physiol Endocrinol Metab. 301(6):E1254-61

* Eschbach J et al. (2011) Mutations in cytoplasmic dynein lead to a Huntington's disease-like defect in energy metabolism of brown and white adipose tissues. Biochim Biophys Acta. 1812(1):59-69

* Wahab F et al. (2010) Effect of Peripheral Kisspeptin Administration on Adiponectin, Leptin, and Resistin Secretion Under Fed and Fasting Conditions in the Adult Male Rhesus Monkey (Macaca mulatta). Horm Metab Res. 42:570-574

* Smirin P et al. (2010) Sarcopoterium spinosum extract as an antidiabetic agent: in vitro and in vivo study. J Ethnopharmacol. 129(1):10-7.

* Hsueh KC et al. (2010) Serum leptin and adiponectin levels correlate with severity of allergic rhinitis. Pediatr Allergy Immunol. 21(1 Pt 2):e155-159

* Ceperuelo-Mallafre V et al. (2009) Circulating and Adipose Tissue Gene Expression of Zinc-alpha2-Glycoprotein in Obesity: Its Relationship with Adipokine and Lipolytic Gene Markers in Subcutaneous and Visceral Fat. J Clin Endocrinol Metab 94(12):5062-5069

* Adali E et al. (2009) Increased visfatin and leptin in pregnancies complicated by pre-eclampsia. J Matern Fetal Neonatal Med. 22(10):873-879

* Cheng KH et al. (2008) Adipocytokines and proinflammatory mediators from abdominal and epicardial adipose tissue in patients with coronary artery disease. Int J Obes (Lond) 32(2):268-274

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