Mouse Thrombin-Antithrombin (TAT) Complex ELISA Kit

Coagulation and Fibrinolysis Profiles in Mouse Breast Cancer 4T1 Tumor-Bearing Mice: A Characterization of Neutrophil-Associated Prothrombotic States

• https://www.jstage.jst.go.jp/article/bpb/48/12/48_b25-00447/_article/-char/ja/
• R Morimoto-Kamata, L Munakata - Biological and , 2025 - jstage.jst.go.jp
• Cancer-associated thrombosis is the second leading cause of death in patients with cancer. The highly metastatic triple-negative mouse breast cancer cell line 4T1 induces thrombus

Acid sphingomyelinase plays a critical role in LPS-and cytokine-induced tissue factor procoagulant activity

• https://ashpublications.org/blood/article-abstract/134/7/645/260728
• J Wang, UR Pendurthi, LVM Rao - Blood, The Journal of the , 2019 - ashpublications.org
• Tissue factor (TF) is a cofactor for factor VIIa and the primary cellular initiator of coagulation. Typically, most TF on cell surfaces exists in a cryptic coagulant-inactive state but are

Leukocytes as a reservoir of circulating oncogenic DNA and regulatory targets of tumor-derived extracellular vesicles

• https://www.sciencedirect.com/science/article/pii/S1538783622023595
• S Chennakrishnaiah, B Meehan, E D'asti - Journal of Thrombosis , 2018 - Elsevier
• Essentials Tumor-bearing mice were employed to follow oncogenic HRAS sequences in plasma, and blood cells. Cancer DNA accumulated in leukocytes above levels detected in

Platelet factor 4 limits neutrophil extracellular trapand cell-free DNAinduced thrombogenicity and endothelial injury

• https://pmc.ncbi.nlm.nih.gov/articles/PMC10721321/
• ATP Ngo, A Skidmore, J Oberg, I Yarovoi, A Sarkar - JCI , 2023 - pmc.ncbi.nlm.nih.gov
• To assess thrombin generation in murine septic plasma, TAT complexes were measured by ELISA kit (AssayPro, EMT1020-1) as follows: citrated plasma was diluted 125-fold and

Enzymatic lipid oxidation by eosinophils propagates coagulation, hemostasis, and thrombotic disease

• https://rupress.org/jem/article-abstract/214/7/2121/52170
• S Uderhardt, JA Ackermann, T Fillep - Journal of Experimental , 2017 - rupress.org
• Blood coagulation is essential for physiological hemostasis but simultaneously contributes to thrombotic disease. However, molecular and cellular events controlling initiation and

Vascular protein disulfide isomerase A1 mediates endothelial dysfunction induced by angiotensin II in mice

• https://onlinelibrary.wiley.com/doi/abs/10.1111/apha.14116
• A Kij, A Bar, I Czyzynska-Cichon - Acta , 2024 - Wiley Online Library
• Aim Protein disulfide isomerases (PDIs) are involved in platelet aggregation and intravascular thrombosis, but their role in regulating endothelial function is unclear. Here, we

Extracellular VesicleMediated Trafficking of Oncogenic DNA in the Tumour Microenvironment and Biofluids

• https://search.proquest.com/openview/59d3c6fe4b8cd6a3b55e16301199182c/1?pq-origsite=gscholar&cbl=44156
• S Chennakrishnaiah - 2019 - search.proquest.com
• Extracellular vesicles (EVs), including exosomes, are membrane bound cellular fragments that have emerged as mediators of intercellular communication and reservoirs of diagnostic

Therapeutic doses of recombinant factor VIIa in hemophilia generates thrombin in platelet-dependent and -independent mechanisms

• https://onlinelibrary.wiley.com/doi/epdf/10.1111/jth.14881
• Keshava S et al. (2020). J Thromb Haemost. 18(8):1911-1921.
• No Snippet Provided

Acid sphingomyelinase plays a critical role in LPS- and cytokine-induced tissue factor procoagulant activity

• https://ashpublications.org/blood/article-abstract/134/7/645/260728
• Wang J et al. (2019). Blood 134 (7): 645-655.
• No Snippet Provided

Maintaining extraembryonic expression allows generation of mice with severe tissue factor pathway inhibitor deficiency

• http://www.bloodadvances.org/content/bloodoa/3/3/489.full.pdf?sso-checked=true
• Castillo MM et al. (2019). Blood Adv. 3(3): 489?498
• No Snippet Provided

Angiotensin II promotes the anticoagulant effects of rivaroxaban via angiotensin type 2 receptor signaling in mice

• https://www.nature.com/articles/s41598-017-00473-5
• Yang D et al. (2017). Sci Rep. 7(1):369
• No Snippet Provided

Protease-activated receptor 2 exacerbates adenine-induced renal tubulointerstitial injury in mice

• https://pubmed.ncbi.nlm.nih.gov/28025140/#affiliation-1
• Hayashi S et al. (2017).Biochem Biophys Res Commun. 483(1):547-552.
• No Snippet Provided

Citrulline supplementation attenuates the development of non-alcoholic steatohepatitis in female mice through mechanisms involving intestinal arginase

• https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868995/pdf/main.pdf
• Rajcic D et al. (2021). Redox Biol. 41:101879.
• No Snippet Provided

Protective effect of proanthocyanidins on nephrotoxicity induced by antitumor dose of cisplatin in ehrlich solid tumor-bearing mice. Arab

• https://www.researchgate.net/publication/273523860_PROTECTIVE_EFFECT_OF_PROANTHOCYANIDINS_ON_NEPHROTOXICITY_INDUCED_BY_ANTITUMOR_DOSE_OF_CISPLATIN_IN_EHRLICH_SOLID_TUMOR-BEARING_MICE
• Salman TM et al. (2014). J. Lab. Med. 40(2):953-965.
• No Snippet Provided

Anti-Inflammatory Activity of Pistacia khinjuk in Different Experimental Models: Isolation and Characterization of Its Flavonoids and Galloylated Sugars

• https://pubmed.ncbi.nlm.nih.gov/22082098/#affiliation-1
• Esmat A et al. (2012). J Med Food. 15(3):278-287
• No Snippet Provided

Evaluation of the Effects of a High Dose of Erythropoietin-beta on Early Endotoxemia Using a Rat Model

• https://pubmed.ncbi.nlm.nih.gov/23214195/
• Eren Z et al. (2012). Adv Clin Exp Med. 21(3):321?329
• No Snippet Provided

Nature of Fatty Acids in High Fat Diets Differentially Delineates Obesity-Linked Metabolic Syndrome Components in Male and Female C57BL/6J Mice

• http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3277487/pdf/1758-5996-3-34.pdf
• El Akoum S et al. (2011). Diabetol Metab Syndr. 3(1):34
• No Snippet Provided

Platelet factor 4 modulates endothelial cell antimicrobial activity to enhance bacterial clearance and improve sepsis outcomes

• https://pubmed.ncbi.nlm.nih.gov/40950105/
• Ngo Anh T. P., Ortmann Weronika, ..., Gollomp Kandace (2025). bioRxiv. 2025 Sep 03
• PrePrint: elative protein concentration was expressed as NPX (log 2 normalized protein expression). To assess thrombin generation in septic plasma, thrombin anti-thrombin (TAT) complexes were measured by ELISA (AssayPro; EMT1020-1) according to manufacturer’s instructions. Briefly, EDTA-plasma was diluted 110-fold and incubated for 2 hr at RT in 96-well plates pre-coated with polyclonal antibody against mous

Preclinical Evaluation of the Systemic Safety, Efficacy, and Biodistribution of a Recombinant AAV8 Vector Expressing FIX-TripleL in Hemophilia B Mice: Implications for Human Gene Therapy

• https://pubmed.ncbi.nlm.nih.gov/40649847/
• Sheng-Chieh Chou, Cheng-Po Huang, ..., Maria Valeria Catani (2025). International Journal of Molecular Science…. 2025 Jun 24
• Plasma TAT concentrations were quantified using a commercially available Mouse TAT ELISA Kit (Assaypro LLC, St. Charles, MO, USA).. Briefly, diluted plasma samples were added to 96-well plates precoated with a TAT-specific antibody.Briefly, ..

Low phylloquinone intake deteriorates endothelial function in normolipidemic and dyslipidaemic mice.

• https://pubmed.ncbi.nlm.nih.gov/39978646/
• Agnieszka Kij, Anna Kieronska-Rudek, ..., Stefan Chlopicki (2025). The Journal of nutritional biochemistry. 2025 Feb 18
• The effect of PK-deficient diets on blood coagulation was assessed based on the plasma concentration of the thrombin-antithrombin (TAT) complex quantified using a commercially available enzyme-linked immunosorbent assay (ELISA) kit (EMT1020-1; AssayPro, St. Charles, MO, USA) according to the manufacturer’s instructions.. Thrombin generation was measured using calibrated automated thrombography (CAT) as ..

Vascular protein disulfide isomerase A1 mediates endothelial dysfunction induced by angiotensin II in mice.

• https://pubmed.ncbi.nlm.nih.gov/38400621/
• Agnieszka Kij, Anna Bar, ..., Stefan Chlopicki (2024). Acta physiologica (Oxford, England). 2024 Apr 17
• .. into thrombin concentration as described previously.77 All reagents were gifted by Synapse BV (Netherlands).. The concentration of the thrombin–antithrombin complex was assessed with a commercially available ELISA kit (EMT1020- 1; AssayPro, St. Charles, MO, USA) according to the attached instructions.. The platelet activity was also evaluated based on the level of thromboxane B2 (TXB2; ..

Platelet factor 4 limits neutrophil extracellular trap- and cell-free DNA-induced thrombogenicity and endothelial injury.

• https://pubmed.ncbi.nlm.nih.gov/37991024/
• Anh Tp Ngo, Abigail Skidmore, ..., Kandace Gollomp (2023). JCI insight. 2023 Nov 23
• .. isolated by centrifugation at 2000g for 20 minutes at RT.. To assess thrombin generation in murine septic plasma, TAT complexes were measured by ELISA kit (AssayPro, EMT1020-1) as follows: citrated plasma was diluted 125-fold and incubated for 2 hours at RT in 96-well plates precoated with polyclonal antibody against mouse thrombin.. The wells were washed and incubated with biotinylated mouse TAT ..

Neutrophil extracellular trap stabilization by platelet factor 4 reduces thrombogenicity and endothelial cell injury

• https://pubmed.ncbi.nlm.nih.gov/36711969/
• Ngo Anh T. P., Sarkar Amrita, ..., Gollomp Kandace (2023). bioRxiv. 2023 Jan 09
• PrePrint: .. and platelet-poor plasma was isolated by centrifugation at 2000g for 20 minutes at RT.. Thrombin anti-thrombin complexes in septic plasma were measured using commercially available ELISA kits (AssayPro; EMT1020-1) according to manufacturers’ instructions.. Briefly, citrated plasma was diluted 125-fold and incubated with polyclonal antibody against mouse thrombin ..

Alterations to Sphingomyelin Metabolism Affect Hemostasis and Thrombosis

• https://pubmed.ncbi.nlm.nih.gov/36412194/
• Jue Wang, Shiva Keshava, ..., L. Vijaya Mohan Rao (2023). Arteriosclerosis, thrombosis, and vascular…. 2023 Jan 01
• Thrombin-antithrombin complex levels in the mouse plasma were determined using AssayMax mouse thrombin-antithrombin complex ELISA kit (Assaypro LLC, MO 63301) and following the protocol included in the kit.

Circulatory neutrophils exhibit enhanced neutrophil extracellular trap formation in early puerperium: NETs at the nexus of thrombosis and immunity?

• https://pubmed.ncbi.nlm.nih.gov/34948443/
• Giaglis Stavros, Chowdhury Chanchal Sur (2021). bioRxiv. 2021 Oct 18
• PrePrint: To detect NET-associated MPO/DNA complexes, a modified capture ELISA was utilized ( ).. The concentrations of thrombin-antithrombin (TAT) complexes and D-dimers were measured by sandwich ELISA, utilizing the human TAT Complexes ELISA Kit (Assaypro) and the Imuclone D-Dimer ELISA Kit (American Diagnostica) respectively.. RNA Isolation and Quantitative Real-time PCR Total RNA was isolated from 3×106 neutrophils by ..

Therapeutic doses of recombinant factor VIIa in hemophilia generates thrombin in platelet-dependent and -independent mechanisms

• https://pubmed.ncbi.nlm.nih.gov/32359012/
• Shiva Keshava, Usha R Pendurthi, ..., L. Vijaya Mohan Rao (2021). Journal of thrombosis and haemostasis : JT…. 2021 Aug 01
• Measurement of TAT levels.Measurement of TAT levels.. Plasma isolated from blood collected at the injury site, via the sub-mandibular vein, or cardiac puncture was analyzed for TAT levels using AssayMax mouse ELISA kit (AssayPro, St. Charles, MO).

Leukocytes as a reservoir of circulating oncogenic DNA and regulatory targets of tumor-derived extracellular vesicles.

• https://pubmed.ncbi.nlm.nih.gov/29971917/
• S Chennakrishnaiah, B Meehan, ..., J Rak (2019). Journal of thrombosis and haemostasis : JT…. 2019 Dec 23
• od was collected from tumor-bearing and tumor-free mice via inferior vena cava (IVC) using 3.8% sodium citrate as anticoagulant. Plasma was prepared from blood samples (Fig. S3) and analyzed by ELISA (Assaypro, catalog No. EMT1020-1) prior to humane sacrifice of mice. All data were independently reproduced at least 2-3 times and statistically analyzed using two-tailed t-test with A cc ep te d A rt

Acid sphingomyelinase plays a critical role in LPS- and cytokine-induced tissue factor procoagulant activity

• https://pubmed.ncbi.nlm.nih.gov/31262782/
• Jue Wang, Usha R Pendurthi, L Vijaya Mohan Rao (2019). Blood. 2019 Aug 15
• .. murine blood was measured as levels of thrombin-antithrombin (TAT) complexes formed in the blood.. TAT levels were measured using an AssayMax Mouse Thrombin-Antithrombin (TAT) Complex ELISA Kit (AssayPro, St. Charles, MO), following the instructions provided by the manufacturer.. Determination of ASMase levels in plasmaASMase levels in plasma were measured using an Amplex ..

Novel Role for the AnxA1-Fpr2/ALX Signaling Axis as a Key Regulator of Platelet Function to Promote Resolution of Inflammation

• https://pubmed.ncbi.nlm.nih.gov/31329483/
• Elena Y Senchenkova, Junaid Ansari, ..., Felicity N E Gavins (2019). Circulation. 2019 Jul 23
• Thrombin–Antithrombin Complex Measurement Plasma was collected from WT and AnxA1−/− mice, and thrombin–antithrombin was measured using thrombin–antithrombin complex ELISAs (Assay Pro, Saint Charles, MO).

Chitinase 3-like-1 Promotes Intrahepatic Activation of Coagulation Through Induction of Tissue Factor in Mice

• https://pubmed.ncbi.nlm.nih.gov/29251791/
• Zhao Shan, Xiaodong Liu, ..., Cynthia Ju (2019). Hepatology (Baltimore, Md.). 2019 Jun 01
• Abstract: Coagulation is a critical component in the progression of liver disease. Identification of key molecules involved in the intrahepatic activation of coagulation (IAOC) will be instrumental in…

Maintaining extraembryonic expression allows generation of mice with severe tissue factor pathway inhibitor deficiency

• https://pubmed.ncbi.nlm.nih.gov/30755437/
• Michelle M Castillo, Qiuhui Yang, ..., Rashmi Sood (2019). Blood Advances. 2019 Feb 12
• Plasma TAT levels were measured using AssayMax Mouse TAT Elisa Kit (Assaypro LLC, St. Charles, MO) according to the manufacturer’s instructions.. Plasma was prepared by collecting 600 μL blood from the inferior vena cava into ..

Enzymatic lipid oxidation by eosinophils propagates coagulation, hemostasis, and thrombotic disease

• https://pubmed.ncbi.nlm.nih.gov/29463570/
• Stefan Uderhardt, Jochen A. Ackermann, Steffen Massberg (2017). The Journal of Experimental Medicine. 2017 Jul 03
• .. was obtained by 10-min centrifugation (3,000 g ) and stored at −80°C until analysis.. The following ELISA kits were used: Mouse TAT Complex ELISA kit (EMT1020-1; Assaypro) and Human ECP ELISA kit (SK00128-01; Aviscera Bioscience).. ELISAs were performed according to the manufacturer’s specifications.

Angiotensin II promotes the anticoagulant effects of rivaroxaban via angiotensin type 2 receptor signaling in mice

• https://pubmed.ncbi.nlm.nih.gov/28337024/
• Dan Yang, Junjie Shao, Ping Xie (2017). Scientific Reports. 2017 Mar 23
• .. mice were anesthetized with isoflurane gas, and blood was collected with a cardiac puncture.. Plasma thrombin-antithrombin (TAT) complexes were assayed through AssayMax mouse ELISA (AssayPro, St. Charles, MO).. Plasma TF and TFPI levels were determined with ImubindTM total TF and TFPI ELISA ..

Coagulation factor XII induces pro-inflammatory cytokine responses in macrophages and promotes atherosclerosis in mice

• https://pubmed.ncbi.nlm.nih.gov/27786338/
• Helga D. Manthey, Clement Cochain (2017). Thrombosis and haemostasis. 2017 Jan 01
• Abstract: Atherosclerosis is considered a chronic inflammatory disease of the vessel wall. Coagulation pathways and immune responses contribute to disease development. The role of coagulation factor X…

Protease–activated receptor 2 exacerbates adenine–induced renal tubulointerstitial injury in mice

• https://pubmed.ncbi.nlm.nih.gov/28025140/
• Sakiko Hayashi, Yuji Oe, ..., Nobuyuki Takahashi (2017). Biochemical and biophysical research commu…. 2017 Jan 01
• Abstract: Hypercoagulability is associated with chronic kidney disease (CKD). Tissue factor/factor VIIa complex and factor Xa in the coagulation cascade are known to activate protease-activated recept…

Thrombin inhibition and cyclophosphamide synergistically block tumor progression and metastasis

• https://pubmed.ncbi.nlm.nih.gov/26383051/
• Eric T Alexander, Allyson R Minton, ..., Susan K Gilmour (2015). Cancer Biology & Therapy. 2015 Sep 18
• .. cells as well as staining for Annexin V and TF as described above. ... PPP samples were diluted 1:4 and then assayed with the mouse TAT complex ELISA kit (AssayPro) as per the manufacturer's instructions.. The absorbance was read on a microplate reader at a wavelength of 450 nm, ..

Toll-Like Receptor 9 Signaling Regulates Tissue Factor and Tissue Factor Pathway Inhibitor Expression in Human Endothelial Cells and Coagulation in Mice

• https://pubmed.ncbi.nlm.nih.gov/25855902/
• Driss El Kebir, Anas Damlaj, ..., János G. Filep (2015). Critical Care Medicine. 2015 Jun 01
• .. red.Blood monocyte count was determined following lysis of RBCs and staining with neutral red.. Plasma was analyzed for thrombin-antithrombin (TAT) complexes using AssayMax mouse ELISA (AssayPro, St. Charles, MO).. The intra-assay and inter-assay coefficients of variation were less than 5% and 7%, respectively.The ..

Factor VIIa binding to endothelial cell protein C receptor protects vascular barrier integrity in vivo

• https://pubmed.ncbi.nlm.nih.gov/24977291/
• J Sundaram, S Keshava, ..., L V M Rao (2015). Journal of thrombosis and haemostasis : JT…. 2015 May 01
• Images were captured with an Olympus DP25 camera.. Mouse TAT complex levels were measured with the mouse TAT Complex ELISA Kit (Assaypro, St Charles, MO, USA).. As shown in our earlier study [ ], LPS administration markedly increased vascular leakage ..

Role of Tissue Factor in Mycobacterium tuberculosis-Induced Inflammation and Disease Pathogenesis

• https://pubmed.ncbi.nlm.nih.gov/25462128/
• Hema Kothari, Shiva Keshava, Usha R. Pendurthi (2014). PLoS ONE. 2014 Dec 02
• Cytokine ELISA kits were from eBioscience (San Diego, CA).. Mouse TAT-complex ELISA kit was from Assaypro (St. Charles, MO).. Mouse macrophage marker antibody F4/80 (SP115) was from Novus Biologicals (Littleton, CO) and neutrophil ..

Stability of coagulation factors in plasma prepared after a 24-hour room temperature hold

• https://pubmed.ncbi.nlm.nih.gov/20412526/
• Hiba Alhumaidan, Tracey Cheves (2010). Transfusion. 2010 Apr 15
• Abstract: The manufacture of fresh-frozen plasma (FFP) requires that plasma be frozen within 8 hours of collection and 24-hour frozen plasma requires 1 to 6°C refrigeration before freezing. Manufactur…

The Role of Fibrinolytic and Antifibrinolytic Activities in the Pathophysiology of HELLP Syndrome

• https://pubmed.ncbi.nlm.nih.gov/19943771/
• Suleyman Guven, Mehmet Sonmez (2009). Hypertension in pregnancy. 2009 Nov 27
• Abstract: To determine the role of the fibrinolytic and antifibrinolytic systems in HELLP syndrome. This study consisted of patients with HELLP Syndrome (study group, n = 17), women who were admitted …