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AssayLite ™ Fluorescent Singleplex Kits
The AssayLite™ series is based on the Sandwich Immunoassay or Competition Immunoassay principles.
I. Competition Fluorescent Immunoassay principles.
Step 1: A specific antibody coated on the plate uniformly and standard and/or sample along with fluorescent labeled protein are added to the well.
Step 2: Fluorescent labeled protein compete with none labeled protein binding to the antibody. The blank has the highest fluorescent signal and the signal decreases when the non-labeled protein concentration increases.
II. Sandwich Fluorescent Immunoassay principles.
Step 1: A specific antibody coated on the plate uniformly and standard and/or sample are added to the well.
Step 2: Targeted antibody bind to the standard and/or sample.
Step 3: Antibody labeled with fluorescent probe is added to the well.
Step 4: Antibody with fluorescent probe binds to the targeted protein and the specific fluorescent signal is read by fluorescent plate reader.